Evolutionarily conserved chaperone-mediated proteasomal degradation of a disease-linked aspartoacylase variant

Abstract

Canavan disease is a severe progressive neurodegenerative disorder that is characterized by swelling and spongy degeneration of brain white matter. The disease is genetically linked to polymorphisms in the aspartoacylase (ASPA) gene, including the substitution C152W. ASPA C152W is associated with greatly reduced protein levels in cells, yet biophysical experiments suggest a wild-type like thermal stability. Here, we examine the stability and degradation pathway of ASPA C152W. When we expressed ASPA C152W in Saccharomyces cerevisiae, we found a decreased steady state compared to wild-type ASPA as a result of increased proteasomal degradation. However, molecular dynamics simulations of ASPA C152W did not substantially deviate from wild-type ASPA, indicating that the native state is structurally preserved. Instead, we suggest that the C152W substitution prevents ASPA from reaching its stable native conformation, presumably by impacting on de novo folding. Systematic mapping of the protein quality control components acting on misfolded and aggregation-prone species of C152W, revealed that the degradation is highly dependent on the molecular chaperone Hsp70, its co-chaperone Hsp110 as well as several quality control E3 ubiquitin-protein ligases, including Ubr1. In human cells, ASPA C152W displayed increased proteasomal turnover that was similarly dependent on Hsp70 and Hsp110. We propose that Hsp110 is a potential therapeutic target for misfolding ASPA variants that trigger Canavan disease due to excessive degradation.

Galactosemia: Towards Pharmacological Chaperones

“…This is reasonable because the destabilizing effect of mutations required for triggering protein degradation is rather low (about 8–12 kJ·mol −1 ), well within the achievable ligand-induced stabilization [ 114 , 115 , 116 , 117 , 118 , 119 ]. In other cases, protein degradation may depend on local destabilization of the protein structure, which initiates degradation, rather than in global destabilization [ 120 , 121 , 122 , 123 ]. Consequently, a disease-associated variant may bind and become globally (i.e.…”