Triboelectrostatic separation is a promising method used to separate non-conductive minerals. However, the knowledge about the underlying triboelectrification mechanisms is still very limited. Thus, predicting the separation results and finding proper separation parameters are challenging tasks. This article presents a comprehensive summary of phenomena and factors which play a decisive role in the charging behavior of non-conductors and, by implication, the efficiency of the separation process, such as water and adsorbents layers on the surface, surface roughness, humidity, type of contact, etc. The authors hope that this article opens a way for a systematic approach through basic experiments dedicated to a better understanding of triboelectrification processes.