Evolutionary aspects of the ZZ/ZW sex chromosome system in the Characidae fish, genus Triportheus. A monophyletic state and NOR location on the W chromosome

Artoni, Roberto Ferreira; Bertollo, L.A.C.

doi:10.1038/sj.hdy.6800081

Cited by 67 publications

(74 citation statements)

References 33 publications

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“…The Z chromosomes have additional heterochromatin in one or both telomeric regions, depending on the species, whereas the W chromosomes were almost entirely heterochromatic, except for the p arms in all species (Figure 1), in agreement with previous studies (Artoni and Bertollo, 2002;Diniz et al, 2009;Yano et al, 2014). The two-color FISH with 18S and 5S rDNA showed that both sites are colocalized on the p arms of the chromosome pair 3 in T. signatus, with an 18S rDNA additional site adjacent to the Wq telomere (Figure 2).…”

Section: Resultssupporting

confidence: 82%

“…In fact, both techniques were critical to demonstrate the sequences that are still shared by both sex chromosomes or, otherwise, that those are more exclusive to one of them. Noteworthy, it was evidenced that the end of the Wq has a high concentration of female-specific sequences (Figures 2-4), where an 18S rDNA cluster is also located in all Triportheus species so far analyzed (Artoni and Evolution of sex chromosomes in congeneric species CF Yano et al Bertollo, 2002;Nirchio et al, 2007;Diniz et al, 2009;Marquioni et al, 2013;Yano et al, 2014). Sex chromosomes carrying 18S rDNA sequences have already been reported in several other vertebrates, such as Characidium fishes (Scacchetti et al, 2015), cane toad Bufo marinus (Abramyan et al, 2009), Chinese softshell turtle Pelodiscus sinensis (Kawai et al, 2007) and tiger snake Notechis scutatus (O'Meally et al, 2010).…”

Section: Chromosomal Rearrangements and Sex Chromosome Differentiationmentioning

confidence: 99%

“…The Z is a metacentric chromosome, the largest one in the karyotype, whereas the W is always smaller than the Z, and varies in size and morphology among species (Bertollo and Cavallaro, 1992;Sánchez and Jorge, 1999;Artoni et al, 2001;Artoni and Bertollo, 2002;Nirchio et al, 2007;Diniz et al, 2008;Yano et al, 2014Yano et al, , 2016. Besides, the W chromosome is rich in heterochromatin and carries an 18S ribosomal DNA (rDNA) site on its long arms (Artoni and Bertollo, 2002;Nirchio et al, 2007;Diniz et al, 2009;Marquioni et al, 2013;Yano et al, 2014;Schmid et al, 2016). Recently, a molecular phylogeny of the Triportheidae family was introduced based on the 16S rRNA and cytochrome b (CytB) mitochondrial genes, and on the recombination activating gene 1 (Rag1), recombination activating gene 2 (Rag2) and myosin heavy chain 6 cardiac muscle-α (Myh6) nuclear genes (Mariguela et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Highly conserved Z and molecularly diverged W chromosomes in the fish genus Triportheus (Characiformes, Triportheidae)

et al. 2016

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The main objectives of this study were to test: (1) whether the W-chromosome differentiation matches to species' evolutionary divergence (phylogenetic concordance) and (2) whether sex chromosomes share a common ancestor within a congeneric group. The monophyletic genus Triportheus (Characiformes, Triportheidae) was the model group for this study. All species in this genus so far analyzed have ZW sex chromosome system, where the Z is always the largest chromosome of the karyotype, whereas the W chromosome is highly variable ranging from almost homomorphic to highly heteromorphic. We applied conventional and molecular cytogenetic approaches including C-banding, ribosomal DNA mapping, comparative genomic hybridization (CGH) and cross-species whole chromosome painting (WCP) to test our questions. We developed Z-and W-chromosome paints from T. auritus for cross-species WCP and performed CGH in a representative species (T. signatus) to decipher level of homologies and rates of differentiation of W chromosomes. Our study revealed that the ZW sex chromosome system had a common origin, showing highly conserved Z chromosomes and remarkably divergent W chromosomes. Notably, the W chromosomes have evolved to different shapes and sequence contents within~15-25 Myr of divergence time. Such differentiation highlights a dynamic process of W-chromosome evolution within congeneric species of Triportheus.

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Section: Resultssupporting

confidence: 82%

Section: Chromosomal Rearrangements and Sex Chromosome Differentiationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Highly conserved Z and molecularly diverged W chromosomes in the fish genus Triportheus (Characiformes, Triportheidae)

et al. 2016

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“…FISH with rDNA probes has helped detect the presence of inactive NORs in fish chromosomes in different cases, such as in the association of NORs and sex chromosomes (Artoni & Bertollo, 2002) and in cases of multiple silent loci (Centofante et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Cytogenetics of two sympatric Corydoras species (pisces, siluriformes, challichtyidae) of Southern Brazil

et al. 2006

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Karyotypic data are presented for two sympatric Corydoras species of the Lagoa Dourada, namely, C. ehrhadti and C. paleatus, which are found in the upper Tibagi river basin (Ponta Grossa, State of Paraná, Brazil). The same diploid number and karyotypic formula were observed in both species/populations. A great similarity in the constitutive heterochromatin distribution and in the activity of nucleolar organizer regions was also found. The use of in situ hybridization with a fluorescent 18S rDNA probe allowed for the identification of the species/populations through the location of ribosomal sites.Keywords: karyotype, sympatry, heterochromatin, NORs, rDNA. RESUMO Citogenética de espécies simpátricas de Corydoras (pisces, siluriformes, challichtyidae) do Sul do BrasilDados cariotípicos são apresentados para duas espécies simpátricas de Corydoras da Lagoa Dourada, C. ehrhadti e C. paleatus, pertencentes à bacia do alto Rio Tibagi (Ponta Grossa, Paraná, Brasil). O mesmo número diplóide e fórmula cariotípica foram observados em ambas espécies/populações. Grande similaridade foi verificada também para a distribuição da heterocromatina constitutiva e atividade das regiões organizadoras de nucléolos. O emprego da hibridação in situ com sonda fluorescente de DNAr 18S possibilitou identificar as espécies/populações por meio da localização dos sítios ribossomais. simpatria, heterocromatina, RONs, DNAr. 192 ARTONI, R. F. et al. Palavras

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“…In fish, the location of the 45S rDNA (18S þ 5.8S þ 28S) is an important cytogenetic marker, with some groups having only one pair of NORs (Curimatidae, Anastomidae, Parodontidae, Prochilodontidae, Cichlidae), and others showing multiple NORs (Characidae, Lebiasinidae, Loricariidae, Erythrinidae and Callichthydae), including one located on a sex chromosome (Bertollo and Cavallaro, 1992;Born and Bertollo, 2000;Artoni and Bertollo, 2002). NOR localization can be carried out directly with fluorescent in situ hybridization (FISH) with specific probes, or indirectly with the use of silver nitrate (Ag-NOR).…”

Section: Introductionmentioning

confidence: 99%

Karyotypic diversity and evolution of Loricariidae (Pisces, Siluriformes)

et al. 2004

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We present cytogenetic analyses of four fish species, belonging to four Loricariidae subfamilies: Neoplecostomus microps (Neoplecostominae) with 2n ¼ 54 chromosomes, Harttia loricariformis (Loricariinae) with 2n ¼ 56 chromosomes, Hypostomus affinis (Hypostominae) with 2n ¼ 66 chromosomes and Upsilodus sp. (Upsilodinae), with 2n ¼ 96 chromosomes. In addition to karyotypes, data on the location of 18s rDNA sites are presented, derived from indirect (silver nitrate impregnation) and direct (FISH) methods. There is only one pair of nucleolar organizing regions (NORs) per species, except in H. affinis. Diversity and NOR macrokaryotypic evolution in the species analyzed are discussed in relation to the evolution of the Loricariidae as a whole. In addition, a revision of the cytogenetic data available for this family is presented.

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Evolutionary aspects of the ZZ/ZW sex chromosome system in the Characidae fish, genus Triportheus. A monophyletic state and NOR location on the W chromosome

Cited by 67 publications

References 33 publications

Highly conserved Z and molecularly diverged W chromosomes in the fish genus Triportheus (Characiformes, Triportheidae)

Highly conserved Z and molecularly diverged W chromosomes in the fish genus Triportheus (Characiformes, Triportheidae)

Cytogenetics of two sympatric Corydoras species (pisces, siluriformes, challichtyidae) of Southern Brazil

Karyotypic diversity and evolution of Loricariidae (Pisces, Siluriformes)

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