2013
DOI: 10.1111/jnc.12294
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Evolutionary conservation of an atypical glucocorticoid‐responsive element in the human tyrosine hydroxylase gene

Abstract: The human tyrosine hydroxylase (hTH) gene has a 42 bp evolutionarily conserved region designated (CR) II at À7.24 kb, which bears 93% homology to the region we earlier identified as containing the glucocorticoid response element, a 7 bp activator protein-1 (AP-1)-like motif in the rat TH gene. We cloned this hTH-CRII region upstream of minimal basal hTH promoter in luciferase (Luc) reporter vector, and tested glucocorticoid responsiveness in human cell lines. Dexamethasone (Dex) stimulated Luc activity of hTH-… Show more

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Cited by 9 publications
(6 citation statements)
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“…The tissue immunofluorescence confirmed increased density of TH immune-staining at the level of A1/C1 area in the caudal brainstem. Remarkably, the Th gene owns highly conserved glucocorticoid-responsive elements [41,42]. In the present study, the susceptibility of the caudal compared with rostral brainstem to corticosterone-induced TH expression was found to be consistently related to the impressive expression of glucocorticoid receptors in the caudal brainstem compared with the rostral brainstem.…”
Section: Discussionsupporting
confidence: 53%
“…The tissue immunofluorescence confirmed increased density of TH immune-staining at the level of A1/C1 area in the caudal brainstem. Remarkably, the Th gene owns highly conserved glucocorticoid-responsive elements [41,42]. In the present study, the susceptibility of the caudal compared with rostral brainstem to corticosterone-induced TH expression was found to be consistently related to the impressive expression of glucocorticoid receptors in the caudal brainstem compared with the rostral brainstem.…”
Section: Discussionsupporting
confidence: 53%
“…This sequence is almost identical to the GR footprint identified by our structural analysis (Figure 5 ). Binding at this site is conserved in humans, suggesting GR DNA-binding-dependent mechanisms at TREs may represent an evolutionary conserved model ( 64 ).…”
Section: Discussionmentioning
confidence: 99%
“…It is commonly supposed that low epinephrine and PNMT concentrations may arise from a disruption of cellular communication between pheochromocytes from PGL and adrenal cortex. Low GC level may not be able to transactivate a large array of genes that possess GC-binding sites including PNMT [ 6 12 ] and TH [ 17 ]. However, when PHEO were divided into two groups, according to tissue CAT NE/E ratio, a different scenario took shape; PGL and NorAd PHEO featured similar values without significant differences regarding all aspects studied (tissue and plasma CAT concentration as well as gene and protein expression involved in CAT synthesis).…”
Section: Discussionmentioning
confidence: 99%