Evolutionary conservation of the allosteric activation of factor VIIa by tissue factor in lamprey: reply

Abstract

20 Madsen JJ, Persson E, Olsen OH. Tissue factor activates allosteric networks in factor VIIa through structural and dynamic changes. J Thromb Haemost 2015; 13: 262-7. 21 Banner DW, D'Arcy A, Ch ene C, Winkler FK, Guha A, Konigsberg WH, Nemerson Y, Kirchhofer D. The crystal structure of the complex of blood coagulation factor VIIa with soluble tissue factor.

Evolutionary conservation of the allosteric activation of factor VIIa by tissue factor in lamprey: comment

“…We read with great interest the article on allostery in a protease from the blood coagulation cascade by Beeler et al [1] in a recent issue of the Journal of Thrombosis and Haemostasis. Herein, the authors conclude that the allosteric activation of coagulation factor VIIa (FVIIa) by tissue factor (TF) in lampreys appears to be indistinguishable from, and conserved as compared with, that in humans, meaning that lamprey FVIIa (l-FVIIa) is assumed to be catalytically incompetent unless allosterically activated by lamprey TF (l-TF).…”

“…We have read with interest the letter by Olsen and colleagues in response to our recent article [1]. Therein, we reported the cloning of full-length tissue factor (Tf) and factor VIIa (FVIIa) cDNAs from lamprey Petromyzon marinus, and characterization of structural features in the proteins.…”