Evolutionary conservation of the human nucleolar protein fibrillarin and its functional expression in yeast.

Jansen, Ralf‐Peter; Hurt, Eduard C.; Kern, Hildegard; Lehtonen, Hilkka; Carmo‐Fonseca, Maria; Lapeyre, Bruno; Tollervey, David

doi:10.1083/jcb.113.4.715

Cited by 155 publications

(100 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It also differs from transcription termination factor 1 and ribosomal enhancer binding protein 1, which induce termination of rDNA transcription by binding to the IGS downstream from the 28s rRNA coding sequence in animals (Evers et al, 1995) and yeast (Lang et a]., 1994). NF D does not correspond with abundant nucleolar-protein-like nucleolin, which binds to pre-rRNA (Ghisolfi-Nieto et al, 1996), or fibrillarin, a 34-36-kDa protein that associates with small nucleolar RNAs (Jansen et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Two Ribosomal DNA‐Binding Factors Interact with a Cluster of Motifs on the 5′ External Transcribed Spacer, Upstream from the Primary Pre‐rRNA Processing Site in a Higher Plant

Caparrós-Ruiz

Lahmy

Piersanti

et al. 1997

European Journal of Biochemistry

View full text Add to dashboard Cite

In radish the primary processing site in pre-rRNA has been mapped to a TTTTCGCGC sequence Nuclear genes coding for the 25S, 5.8s and 18s rRNAs are organised as head-to-tail tandem repeats of the rRNA sequences separated by two short spacers and a large intergenic spacer (IGS). Transcription of this unit by RNA polymerase I gives a primary rRNA precursor (pre-rRNA) comprising the rRNA and transcribed spacer sequences. Pre-rRNA processing results in removal of spacer sequences by a combination of endonucleolytic cleavages at specific sites, exonucleolytic RNA degradation, and base modification of rRNAs. All these events occur in the nucleolus (Sollner-Webb and Mougey, 1991 ;Moss and Stefanovsky, 1995).In eucaryotes, ribosomal DNA (rDNA) transcription starts at a major transcription initiation site (TIS) located in the IGS and proceeds through the 5' external transcribed spacer (5' ETS). The first pre-rRNA-processing event occurs in the nascent transcript and corresponds to an endonucleolytic cleavage at a speCorrespondence to M. Echevem'a,

show abstract

Section: Discussionmentioning

confidence: 99%

Two Ribosomal DNA‐Binding Factors Interact with a Cluster of Motifs on the 5′ External Transcribed Spacer, Upstream from the Primary Pre‐rRNA Processing Site in a Higher Plant

Caparrós-Ruiz

Lahmy

Piersanti

et al. 1997

European Journal of Biochemistry

View full text Add to dashboard Cite

show abstract

“…Proteins were separated on SDS-polyacrylamide gels and transferred to nitrocellulose membranes by standard procedures. The following primary antibodies were used: mouse monoclonal anti-tyrosine hydroxylase 22941 (Incstar Corporation), mouse monoclonal anti-SMN (clone 8, Transduction Laboratories) and rabbit polyclonal serum raised against a peptide corresponding to the carboxy-terminal end of human fibrillarin (Jansen et al 1991). Immunoblots were visualized by chemiluminescence (ECL, Amersham).…”

Section: Subcellular Fractionation and Immunoblottingmentioning

confidence: 99%

Targeting SMN to Cajal bodies and nuclear gems during neuritogenesis

et al. 2004

View full text Add to dashboard Cite

Neurite outgrowth is a central feature of neuronal differentiation. PC12 cells are a good model system for studying the peripheral nervous system and the outgrowth of neurites. In addition to the dramatic changes observed in the cytoplasm, neuronal differentiation is also accompanied by striking changes in nuclear morphology. The large and sustained increase in nuclear transcription during neuronal differentiation requires synthesis of a large number of factors involved in pre-mRNA processing. We show that the number and composition of the nuclear subdomains called Cajal bodies and gems changes during the course of N-ras-induced neuritogenesis in the PC12-derived cell line UR61. The Cajal bodies found in undifferentiated cells are largely devoid of the survival of motor neurons (SMN) protein product. As cells shift to a differentiated state, SMN is not only globally upregulated, but is progressively recruited to Cajal bodies. Additional SMN foci (also known as Gemini bodies, gems) can also be detected. Using dual-immunogold labeling electron microscopy and mouse embryonic fibroblasts lacking the coilin protein, we show that gems clearly represent a distinct category of nuclear body.

show abstract

“…Recent studies by Huang et al (1992) suggest that box D may also be required for fibrillarin binding to yeast U14 RNA. Like the stem/loop/stem motif, the fibrillarin protein is highly conserved in evolution; human and yeast fibrillarin are 70% identical, and the human protein can functionally complement a yeast fibrillarin mutant (Jansen et al 1991). Alternatively, another protein common to the family of fibrillarin-associated nucleolar snRNPs, might bind to box C' (and/or box D) within the stem/loop/ stem motif.…”

Section: A New Structural Motif For Nucleolar Snrnasmentioning

confidence: 99%

A small nucleolar RNA is processed from an intron of the human gene encoding ribosomal protein S3.

Tycowski¹,

Shu²,

Steitz³

1993

Genes & Development

182

146

View full text Add to dashboard Cite

A human small nucleolar RNA, identified previously in HeLa cells by anti-fibrillarin autoantibody precipitation and termed RNA X, has been characterized. It comprises two uridine-rich variants (148 and 146 nucleotides}, which we refer to as snRNA U15A and U15B. Secondary structure models predict for both variants a U15-specific stem-loop structure, as well as a new structural motif that contains conserved sequences and can also be recognized in the other fibrillarin-associated nucleolar snRNAs, U3, U14, and RNA Y. The single-copy gene for human U15A has been found unexpectedly to reside in intron 1 of the ribosomal protein $3 gene; the U15A sequence appears on the same strand as the $3 mRNA and does not exhibit canonical transcription signals for nuclear RNA polymerases. U15A RNA is processed in vitro from $3 intron 1 transcripts to yield the correct 5' end with a 5'-monophosphate; the in vitro system requires ATP for 3' cleavage, which occurs a few nucleotides downstream of the mature end. The production of a single primary transcript specifying the mRNA for a ribosomal or nucleolar protein and a nucleolar snRNA may constitute a general mechanism for balancing the levels of nucleolar components in vertebrate cells.

show abstract

Evolutionary conservation of the human nucleolar protein fibrillarin and its functional expression in yeast.

Cited by 155 publications

References 36 publications

Two Ribosomal DNA‐Binding Factors Interact with a Cluster of Motifs on the 5′ External Transcribed Spacer, Upstream from the Primary Pre‐rRNA Processing Site in a Higher Plant

Two Ribosomal DNA‐Binding Factors Interact with a Cluster of Motifs on the 5′ External Transcribed Spacer, Upstream from the Primary Pre‐rRNA Processing Site in a Higher Plant

Targeting SMN to Cajal bodies and nuclear gems during neuritogenesis

A small nucleolar RNA is processed from an intron of the human gene encoding ribosomal protein S3.

Contact Info

Product

Resources

About