Evolutionary conservation of zebrafish linkage group 14 with frequently deleted regions of human chromosome 5 in myeloid malignancies

The zebrafish kidney marrow is considered to be the organ of definitive hematopoiesis, analogous to the mammalian bone marrow. We have sequenced 26,143 ESTs and isolated 304 cDNAs with putative full-length ORF from a zebrafish kidney marrow cDNA library. The ESTs formed 7,742 assemblies, representing both previously identified zebrafish ESTs (56%) and recently discovered zebrafish ESTs (44%). About 30% of these EST assemblies have orthologues in humans, including 1,282 disease-associated genes in the Online Mendelian Inheritance in Man (OMIM) database. Comparison of the effective and regulatory molecules related to erythroid functions across species suggests a good conservation from zebrafish to human. Interestingly, both embryonic and adult zebrafish globin genes showed higher homology to the human embryonic globin genes than to the human fetal͞adult ones, consistent with evo-devo correlation hypothesis. In addition, conservation of a whole set of transcription factors involved in globin gene switch suggests the regulatory network for such remodeling mechanism existed before the divergence of the teleost and the ancestor of mammals. We also carried out whole-mount mRNA in situ hybridization assays for 493 cDNAs and identified 80 genes (16%) with tissue-specific expression during the first five days of zebrafish development. Twenty-six of these genes were specifically expressed in hematopoietic or vascular tissues, including three previously unidentified zebrafish genes: coro1a, nephrosin, and dab2. Our results indicate that conserved genetic programs regulate vertebrate hematopoiesis and vasculogenesis, and support the role of the zebrafish as an important animal model for studying both normal development and the molecular pathogenesis of human blood diseases.

show abstract

“…described (10,11). The criteria for a cDNA to be accepted as having an entire ORF have been described (12).…”

Section: Methodsmentioning

confidence: 99%

Hematopoietic gene expression profile in zebrafish kidney marrow

Song

Sun

Deng

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

142

101

View full text Add to dashboard Cite

show abstract

“…Primer pairs were designed from the 3'UTR of zebrafish cebpa using OILGO 6 software (forward primer: 5'-GGTAAAATCATGCCCATTAGCTGC-3'; reverse primer: 5'-CGGAGCGAGCTTGACTTTTGAA-3'). Zebrafish putative orthologues were identified by the "reciprocal best hit" method, as described previously [24].…”

Section: Cloning and Mappingmentioning

confidence: 99%

Dominant-interfering C/EBPα stimulates primitive erythropoiesis in zebrafish

Liu¹,

Rhodes²,

Deng³

et al. 2007

Experimental Hematology

Self Cite

View full text Add to dashboard Cite

Objective-We investigated the role of CCAAT enhancer-binding protein-α (C/EBPα) during zebrafish embryonic blood development.Methods-Whole-mount mRNA in situ hybridization was performed to determine the spatiotemporal expression pattern of zebrafish cebpa in developing hematopoietic progenitors. A deletion mutation of cebpa (zD420), which mimics the human dominant-negative mutations of C/EBPα, was transfected into CV1 cell line to evaluate its transcriptional activity in vitro and injected into zebrafish embryos at the one-to two-cell stage to examine its effects on primitive hematopoiesis during early zebrafish development.Results-Zebrafish cebpa is expressed in the anterior and posterior lateral plate mesoderm at 12 hours postfertilization, along with scl, pu.1 and gata1 in developing hematopoietic progenitors. In vitro, the deletion mutation of cebpa (zD420) prevents expression of the full-length protein, allowing the expression of truncated isoforms from internal translational initiation sites. As in the human, the truncated zebrafish C/ebpα proteins did not activate the expression of known target granulocytic genes, and in fact, suppressed transactivation that was induced in vitro by the full-length protein.Forced expression of the zD420 mRNA in zebrafish embryos led to an expansion of primitive erythropoiesis, without a discernible effect on granulopoiesis.Conclusion-Expression of the truncated isoforms of cebpa alters the developmental pattern of hematopoietic progenitor cells during embryogenesis.

show abstract

“…While others have employed site-directed mutagenesis and chimeric protein analysis as a way to identify the critical amino acids within the Goodpasture epitopes, we hypothesized that evolutionary changes over 450 million years 33 in the amino acids within the ␣3(IV)NC1 domains might offer insight into the unique amino acid residues that comprise the B-cell epitope for Goodpasture autoantibodies. Comparative type IV collagen NC1 sequence analysis between 9 species from Caenorhabditis elegans to humans, BM extraction, NC1 domain analysis from different species, cloning, and recombinant protein production were used to identify the critical amino acids within the B-cell epitopes of the Goodpasture autoantigen.…”

Section: Introductionmentioning

confidence: 99%

Zebrafish to humans: evolution of the 3-chain of type IV collagen and emergence of the autoimmune epitopes associated with Goodpasture syndrome

MacDonald

Sund

Grant

et al. 2006

Blood

View full text Add to dashboard Cite

Goodpasture syndrome is an autoimmune vascular disease associated with kidney and lung failure, with pathogenic circulating autoantibodies targeted to a set of discontinuous epitope sequences within the noncollagenous domain-1 (NC1) of the ␣3 chain of type IV collagen (␣3(IV)NC1), the Goodpasture autoantigen. We demonstrate that basement membrane extracted NC1 domain preparations from Caenorhabditis elegans, Drosophila melanogaster, and Danio rerio do not bind Goodpasture autoantibodies, while Xenopus laevis, chicken, mouse and human ␣3(IV)NC1 domains bind autoantibodies. The ␣3(IV) chain is not present in C elegans and Drosophila melanogaster, but is first detected in the Danio rerio. Interestingly, native Danio rerio ␣3(IV)NC1 does not bind Goodpasture autoantibodies. Next, we cloned, sequenced, and generated recombinant Danio rerio ␣3(IV)NC1 domain. In contrast to recombinant human ␣3(IV)NC1 domain, there was complete absence of autoantibody binding to recombinant Danio rerio ␣3(IV)NC1. Three-dimensional molecular modeling from existing x-ray coordinates of human NC1 domain suggest that evolutionary alteration of electrostatic charge and polarity due to the emergence of critical serine, aspartic acid, and lysine residues, accompanied by the loss of asparagine and glutamine, contributes to the emergence of the 2 major Goodpasture epitopes on the human ␣3(IV)NC1 domain, as it evolved from the Danio rerio over 450 million years. (Blood. 2006;107: 1908-1915

show abstract

Evolutionary conservation of zebrafish linkage group 14 with frequently deleted regions of human chromosome 5 in myeloid malignancies

Cited by 41 publications

References 50 publications

Hematopoietic gene expression profile in zebrafish kidney marrow

Hematopoietic gene expression profile in zebrafish kidney marrow

Dominant-interfering C/EBPα stimulates primitive erythropoiesis in zebrafish

Zebrafish to humans: evolution of the 3-chain of type IV collagen and emergence of the autoimmune epitopes associated with Goodpasture syndrome

Contact Info

Product

Resources

About