Evolutionary conserved brainstem circuits encode category, concentration and mixtures of taste

Vendrell-Llopis, Nuria; Yaksi, Emre

doi:10.1038/srep17825

Cited by 37 publications

(25 citation statements)

References 66 publications

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“…Series of recent studies highlight the increasing popularity of zebrafish for studying diverse aspects of animal behavior [30][31][32][33][34][35][36][37][38][39], including the more complex behaviors such as learning [25,26,[40][41][42][43][44][45][46] and social behaviors [47,48]. While studying complex behaviors in adult zebrafish allow investigation of brain function in a fully mature brain, larval and juvenile zebrafish has the tremendous advantage of a small and transparent brain, when it comes to studying brain A B C1 T1 C2 T2 T3 C3 T4 C4 T5 T6 control dHb activity [33,38,[49][50][51][52][53][54][55][56][57][58]. Hence, we investigated the ontogeny of a complex operant behavior, namely CPA learning, and showed that the learning performance of young zebrafish improves across development.…”

Section: Discussionmentioning

confidence: 99%

The zebrafish dorsolateral habenula is required for updating learned behaviors

Palumbo

Serneels

Pelgrims

et al. 2019

Preprint

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Operant conditioning requires multiple cognitive processes, such as learning, consolidation, prediction of potential outcomes and decision making. It is less clear how interactions of these processes led to behavioral adaptations that allows animal to cope with changing environment. We first showed that juvenile zebrafish can perform conditioned place avoidance learning, with improving performance across development. Next, we disentangled operant conditioning from contextual fear and anxiety. Our results revealed that animals' decisions and learning performance is shaped by the available information and animals' experience. Ablation of dorsal habenula (dHb), a brain region involved in learning and prediction of outcomes, led to an unexpected improvement in animals learning performance and delayed memory extinction. Interestingly, while the control animals' exhibit rapid adaptation to changing learning rules, dHb ablated animals failed to adapt. Altogether, our results showed that dHb plays a central role in switching animals' strategies while integrating new evidences with prior experience. Eggen, M. Andresen, V. Nguyen, A Nygard and our fish facility support team for technical assistance. We thank Nathalie Jurisch-Yaksi and Stephanie Fore for helpful comments on the text. We thank all Yaksi lab members for stimulating discussions. This work was funded by ERC starting grant 335561 (F.P., R.P., E.Y.) and RCN FRIPRO Research Grant 239973 (E.Y.). Work in the E.Y. lab is funded by the Kavli Institute for Systems Neuroscience at NTNU. AUTHOR CONTRIBUTIONSConceptualization, F.P., E.Y.; Methodology and data, F.P., B.S.; Recording software F.P., R.P.; Data

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Section: Discussionmentioning

confidence: 99%

The zebrafish dorsolateral habenula is required for updating learned behaviors

Palumbo

Serneels

Pelgrims

et al. 2019

Preprint

Self Cite

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“…Odour-response data analyses were performed using custom scripts written in MATLAB (Mathworks) [ 31 ]. Images were aligned, and for each trial, the change in fluorescence (Δ F / F , DFF) relative to a control trial was calculated for each pixel, yielding a series of DFF frames.…”

Section: Methodsmentioning

confidence: 99%

Assaying sensory ciliopathies using calcium biosensor expression in zebrafish ciliated olfactory neurons

Bergboer

Wyatt

Austin‐Tse

et al. 2018

Cilia

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BackgroundPrimary cilia mediate signal transduction by acting as an organizing scaffold for receptors, signalling proteins and ion channels. Ciliated olfactory sensory neurons (OSNs) organize olfactory receptors and ion channels on cilia and generate a calcium influx as a primary signal in odourant detection. In the zebrafish olfactory placode, ciliated OSNs and microvillus OSNs constitute the major OSN cell types with distinct odourant sensitivity.MethodsUsing transgenic expression of the calcium biosensor GCaMP5 in OSNs, we analysed sensory cilia-dependent odour responses in live zebrafish, at individual cell resolution. oval/ift88 mutant and ift172 knockdown zebrafish were compared with wild-type siblings to establish ciliated OSN sensitivity to different classes of odourants.Resultsoval/ift88 mutant and ift172 knockdown zebrafish showed fewer and severely shortened OSN cilia without a reduction in OSN number. The fraction of responding OSNs and response amplitudes to bile acids and food odour, both sensed by ciliated OSNs, were significantly reduced in ift88 mutants and ift172-deficient embryos, while the amino acids responses were not significantly changed.ConclusionsOur approach presents a quantitative model for studying sensory cilia signalling using zebrafish OSNs. Our results also implicate ift172-deficiency as a novel cause of hyposmia, a reduced sense of smell, highlighting the value of directly assaying sensory cilia signalling in vivo and supporting the idea that hyposmia can be used as a diagnostic indicator of ciliopathies.Electronic supplementary materialThe online version of this article (10.1186/s13630-018-0056-1) contains supplementary material, which is available to authorized users.

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“…Using more recent and brighter forms of GCaMPs (e.g., GCAMP5 and GCaMP6) has allowed for the imaging period to be extended beyond 14 dpf, particularly in the habenula [ 17 , 18 , 19 ]. However, to the best of our knowledge imaging from the optic tectum has not been reported for late stage larvae.…”

Section: Introductionmentioning

confidence: 99%

Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish

Bergmann

Santoscoy

Lygdas

et al. 2018

JDB

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The zebrafish is an established model to study the development and function of visual neuronal circuits in vivo, largely due to their optical accessibility at embryonic and larval stages. In the past decade multiple experimental paradigms have been developed to study visually-driven behaviours, particularly those regulated by the optic tectum, the main visual centre in lower vertebrates. With few exceptions these techniques are limited to young larvae (7–9 days post-fertilisation, dpf). However, many forms of visually-driven behaviour, such as shoaling, emerge at later developmental stages. Consequently, there is a need for an experimental paradigm to image the visual system in zebrafish larvae beyond 9 dpf. Here, we show that using NBT:GCaMP3 line allows for imaging neuronal activity in the optic tectum in late stage larvae until at least 21 dpf. Utilising this line, we have characterised the receptive field properties of tectal neurons of the 2–3 weeks old fish in the cell bodies and the neuropil. The NBT:GCaMP3 line provides a complementary approach and additional opportunities to study neuronal activity in late stage zebrafish larvae.

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Evolutionary conserved brainstem circuits encode category, concentration and mixtures of taste

Cited by 37 publications

References 66 publications

The zebrafish dorsolateral habenula is required for updating learned behaviors

The zebrafish dorsolateral habenula is required for updating learned behaviors

Assaying sensory ciliopathies using calcium biosensor expression in zebrafish ciliated olfactory neurons

Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish

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