Evolutionary correlations in flavonoid production across flowers and leaves in the Iochrominae (Solanaceae)

Berardi, Andrea E.; Hildreth, Sherry B.; Helm, Richard F.; Winkel, Brenda S.J.; Smith, Stacey D.

doi:10.1016/j.phytochem.2016.05.007

Cited by 41 publications

(54 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In plant systems, flavonoid‐based pigmentation is a model trait for evo‐devo studies, and our results in Iochrominae suggest that it will provide a powerful system for testing the hypothesis of pleiotropy‐driven selective filtering of mutations. Similar to many flowering plant clades, Iochrominae displays wide inter‐ and intraspecific variation in flower color, much of it driven by differences in flavonoid type and amount . Our flavonoid profiling confirms that the white morphs that appear periodically in populations of normally pigmented species share the same biochemical signature as white species, that is, they lack the colorful anthocyanins while still producing the upstream flavonols (Figures ).…”

Section: Discussionsupporting

confidence: 72%

“…Similar to many flowering plant clades, Iochrominae displays wide inter-and intraspecific variation in flower color, much of it driven by differences in flavonoid type and amount. 20,23,26 Our flavonoid profiling confirms that the white morphs that appear periodically in populations of normally pigmented species share the same biochemical signature as white species, that is, they lack the colorful anthocyanins while still producing the upstream flavonols (Figures 1-3). This biochemical change is tightly associated with downregulation of downstream genes (DFR, ANS) in the white species 20,21 but not in the white morphs (Figures 4 and 5).…”

Section: Discussionsupporting

confidence: 65%

“…Anthocyanin and flavonol content for the white morphs is displayed with bars outlined in gray as indicated in the legend. † Vassobia breviflora and Iochroma confertiflorum produce trace amounts of flavonols (; Larter et al unpublished) although these were not detected in this HPLC analysis. Dunalia solanacea does not make flavonols or anthocyanidins but does make a moderate quantity of luteolin (a flavone); this data is included here descriptively but not in any subsequent analyses.…”

Section: Introductionmentioning

confidence: 75%

See 2 more Smart Citations

Developmental control of convergent floral pigmentation across evolutionary timescales

Larter

Dunbar‐Wallis

Berardi

et al. 2019

Developmental Dynamics

Self Cite

View full text Add to dashboard Cite

Background Convergent phenotypic evolution has been widely documented across timescales, from populations, to species, to major lineages. The extent to which convergent phenotypes arise from convergent genetic and developmental mechanisms remains an open question, although studies to‐date reveal examples of both similar and different underlying mechanisms. This variation likely relates to a range of factors, including the genetic architecture of the trait and selective filtering of mutations over time. Here we focus on floral pigmentation, and examine the degree of developmental convergence between white‐flowered lineages and white morphs within pigmented species. Results Using the model clade Iochrominae, we find that white morphs and white‐flowered species are biochemically convergent, sharing an absence of colorful anthocyanin pigments. Regression analyses suggest that the expression levels of upstream genes are the strongest drivers of total pigmentation across species, although white species also show sharp down‐regulation of the downstream genes. The white morphs do not share this pattern and present overall expression profiles more similar to the pigmented species. Conclusions These results suggest that the mechanisms underlying variation within populations differ from those which give rise to fixed differences between species. Future work will aim to uncover the genetic changes responsible for this developmental non‐convergence.

show abstract

Section: Discussionsupporting

confidence: 72%

Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 75%

See 1 more Smart Citation

Developmental control of convergent floral pigmentation across evolutionary timescales

Larter

Dunbar‐Wallis

Berardi

et al. 2019

Developmental Dynamics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Continuous variation in flower color caused by subtle differences in the composition and concentration of floral pigments is also measured (Papiorek et al, 2013;Tastard, Andalo, Giurfa, Burrus, & Thébaud, 2008). An alternative to reporting a discrete color polymorphism is to use spectrophotometry (Arista, Talavera, Berjano, & Ortiz, 2013;Schemske & Bierzychudek, 2001) or a combination of ocular assessment and spectrophotometry (Berardi, Hildreth, Helm, Winkel, & Smith, 2016;Casper & La Pine, 1984). However, photographic techniques and image analysis can also be useful tools for recording complex floral phenotypes (Brito, Weynans, Sazima, & Lunau, 2015;Del Valle, Gallardo-López, Buide, Whittall, & Narbona, 2018;.…”

Section: Which Floral Traits Are Attractive To Bees?mentioning

confidence: 99%

Sex‐specific floral attraction traits in a sequentially hermaphroditic species

Peach

Liu

Klitgaard

et al. 2020

Ecology and Evolution

View full text Add to dashboard Cite

●Many angiosperms are hermaphroditic and produce bisexual flowers in which male (pollen export) and female (stigma receptivity) functions are separated temporally. This sequential hermaphroditism may be associated with variation in flower size, color, or pattern, all of which may influence pollinator attraction. In this study, we describe variation in these traits across discrete functional sex stages within and between 225 greenhouse‐grown individuals of Clarkia unguiculata (Onagraceae). In addition, to identify the effects of floral phenotype on pollinator attraction in this species, we examine the effects of these floral traits on pollen receipt in ~180 individuals in an experimental field array. ●Petal area, ultraviolet (UV)‐absorbing nectar guide area, and blue and green mean petal reflectance differ significantly across the functional sex stages of C. unguiculata. Male‐ and female‐phase flowers display significantly different pollinator attraction traits. Petal and UV nectar guide area increase as flowers progress from male phase to female phase, while blue reflectance and green reflectance peak during anther maturation. ●In field arrays of C. unguiculata, female‐phase flowers with large UV nectar guides receive more pollen than those with small nectar guides, and female‐phase flowers with high mean blue reflectance values are more likely to receive pollen than those with low blue reflectance. Female‐phase flowers with green mean reflectance values that differ most from background foliage also receive more pollen than those that are more similar to foliage. These findings indicate that components of flower color and pattern influence pollen receipt, independent of other plant attributes that may covary with floral traits. We discuss these results in the context of hypotheses that have been proposed to explain sex‐specific floral attraction traits, and we suggest future research that could improve our understanding of sexual dimorphism in sequentially hermaphroditic species and the evolution of features that promote outcrossing.

show abstract

“…Berardi et al. () investigated flower and leaf anthocyanin profiles of Solanaceae varying in flower color and found no cross‐tissue correlation, but the red mottled leaves and flecked tepals of E. americanum suggest the possibility nonetheless merits investigation in this species.…”

Section: Discussionmentioning

confidence: 99%

On the ecological significance of pollen color: a case study in American trout lily (Erythronium americanum)

Austen

Lin

Forrest

2018

Ecology

View full text Add to dashboard Cite

Evolutionary ecologists seek to explain the processes that maintain variation within populations. In plants, petal color variation can affect pollinator visitation, environmental tolerance, and herbivore deterrence. Variation in sexual organs may similarly affect plant performance. Within-population variation in pollen color, as occurs in the eastern North American spring ephemeral Erythronium americanum, provides an excellent opportunity to investigate the maintenance of variation in this trait. Although the red/yellow pollen-color polymorphism of E. americanum is widely recognized, it has been poorly documented. Our goals were thus (1) to determine the geographic distribution of the color morphs and (2) to test the effects of pollen color on components of pollen performance. Data provided by citizen scientists indicated that populations range from monomorphic red, to polymorphic, to monomorphic yellow, but there was no detectable geographic pattern in morph distribution, suggesting morph occurrence cannot be explained by a broad-scale ecological cline. In field experiments, we found no effect of pollen color on the probability of predation by the pollen-feeding beetle Asclera ruficollis, on the ability of pollen to tolerate UV-B radiation, or on siring success (as measured by the fruit set of hand-pollinated flowers). Pollinators, however, exhibited site-specific pollen-color preferences, suggesting they may act as agents of selection on this trait, and, depending on the constancy of their preferences, could contribute to the maintenance of variation. Collectively, our results eliminate some hypothesized ecological effects of pollen color in E. americanum, and identify effects of pollen color on pollinator attraction as a promising direction for future investigation.

show abstract

Evolutionary correlations in flavonoid production across flowers and leaves in the Iochrominae (Solanaceae)

Cited by 41 publications

References 72 publications

Developmental control of convergent floral pigmentation across evolutionary timescales

Developmental control of convergent floral pigmentation across evolutionary timescales

Sex‐specific floral attraction traits in a sequentially hermaphroditic species

On the ecological significance of pollen color: a case study in American trout lily (Erythronium americanum)

Contact Info

Product

Resources

About