Evolutionary Dynamics of Repetitive DNA in &lt;b&gt;&lt;i&gt;Semaprochilodus &lt;/i&gt;&lt;/b&gt;(Characiformes, Prochilodontidae): A Fish Model for Sex Chromosome Differentiation

Terencio, Maria Leandra; Schneider, Carlos Henrique; Gross, Maria Cláudia; Vicari, Marcelo Ricardo; Farias, Izeni Pires; Passos, Kelmer Batalha; Feldberg, Eliana

doi:10.1159/000356691

Cited by 25 publications

(22 citation statements)

References 38 publications

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“…Coincidently, it is also the only species in our study that exhibited a fully heterochromatic W chromosome, suggesting an association between heterochromatinization and (TTA) 10 accumulation. Consistent with this result, previous studies have shown greater SSR accumulation in the heterochromatic areas of the sex-specific chromosome [Pokorná et al, 2011;Cioffi et al, 2012;Poltronieri et al, 2013;Terencio et al, 2013;Nanda et al, 2014;Yano et al, 2014;Ziemniczak et al, 2014]. However, we found that all other SSRs clustered to either euchromatic or heterochromatic regions of the sex chromosomes, indicating that they are not necessarily associated with heterochromatin accumulation.…”

Section: Discussionsupporting

confidence: 91%

“…In fact, it has been postulated that microsatellite accumulation in eukaryotic genomes is nonrandom and that each group of organisms/species show preferential accumulation of specific SSR motifs with a particular chromosomal distribution [Tóth et al, 2000;Ruiz-Ruano et al, 2015]. The (CA) 15 , (GA) 15 and (CG) 15 SSRs were previously mapped in different characiform fishes and had similar subtelomeric distributions in Hoplias , Leporinus and Triportheus species, while 2 Semaprochilodus species showed few centromeric/subtelomeric signals [Cioffi et al, 2011[Cioffi et al, , 2012Poltronieri et al, 2013;Terencio et al, 2013;Yano et al, 2014].…”

Section: Discussionmentioning

confidence: 99%

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Chromosomal Mapping of Repetitive DNAs in Characidium (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes

Scacchetti

Utsunomia

Pansonato-Alves

et al. 2015

Cytogenet Genome Res

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The speciose neotropical genus Characidium has proven to be a good model for cytogenetic exploration. Representatives of this genus often have a conserved diploid chromosome number; some species exhibit a highly differentiated ZZ/ZW sex chromosome system, while others do not show any sex-related chromosome heteromorphism. In this study, chromosome painting using a W-specific probe and comparative chromosome mapping of repetitive sequences, including ribosomal clusters and 4 microsatellite motifs - (CA)₁₅, (GA)₁₅, (CG)₁₅, and (TTA)₁₀ -, were performed in 6 Characidium species, 5 of which possessed a heteromorphic ZW sex chromosome system. The W-specific probe showed hybridization signals on the W chromosome of all analyzed species, indicating homology among the W chromosomes. Remarkably, a single major rDNA-bearing chromosome pair was found in all species. The 18S rDNA localized to the sex chromosomes in C. lanei, C. timbuiense and C. pterostictum, while the major rDNA localized to one autosome pair in C. vidali and C. gomesi. In contrast, the number of 5S rDNA-bearing chromosomes varied. Notably, minor ribosomal clusters were identified in the W chromosome of C. vidali. Microsatellites were widely distributed across almost all chromosomes of the karyotypes, with a greater accumulation in the subtelomeric regions. However, clear differences in the abundance of each motif were detected in each species. In addition, the Z and W chromosomes showed the differential accumulation of distinct motifs. Our results revealed variability in the distribution of repetitive DNA sequences and their possible association with sex chromosome diversification in Characidium species.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Chromosomal Mapping of Repetitive DNAs in Characidium (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes

Scacchetti

Utsunomia

Pansonato-Alves

et al. 2015

Cytogenet Genome Res

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“…Similar patterns of random microsatellite distribution have been recently reported in other Orthopteran species, i.e., the grasshoppers Abracris flavolineata (Milani and Cabral-de-Mello 2014) and Ronderosia bergi (Palacios-Gimenez et al in preparation), although for some repeats, in addition to disperse signals, specific enriched regions were also observed, indicating distinct patterns of accumulation at the intragenomic and interspecific level. Similar dispersal patterns of microsatellite arrays and specific localization in sex chromosomes have been previously reported in other eukaryotes, such as plants (Kubat et al 2008;Kejnovský et al 2013), reptiles (Pokorná et al 2011;Matsubara et al 2013) and fish (Terencio et al 2013), and some authors have highlighted a role for this class of sequence in sex chromosome differentiation (see for example Kubat et al 2008;Pokorná et al 2011;Kejnovský et al 2013;Matsubara et al 2013).…”

Section: Discussionsupporting

confidence: 77%

Repetitive DNA chromosomal organization in the cricket Cycloptiloides americanus: a case of the unusual X1X20 sex chromosome system in Orthoptera

Palacios-Gimenez

Cabral-de-Mello

2014

Mol Genet Genomics

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A common placement for most sex chromosomes that is involved in their evolutionary histories is the accumulation of distinct classes of repetitive DNAs. Here, with the aim of understanding the poorly studied repetitive DNA organization in crickets and its possible role in sex chromosome differentiation, we characterized the chromosomes of the cricket species Cycloptiloides americanus, a species with the remarkable presence of the unusual sex chromosome system X1X20♂/X1X1X2X2♀. For these proposes, we used C-banding and mapping through the fluorescence in situ hybridization of some repetitive DNAs. The C-banding and distribution of highly and moderately repetitive DNAs (C 0t-1 DNA) varied depending of the chromosome. The greater accumulation of repetitive DNAs in the X2 chromosome was evidenced. The microsatellites were spread along entire chromosomes, but (AG)10 and (TAA)10 were less enriched, mainly in the centromeric areas. Among the multigene families, the 18S rDNA was spread throughout almost all of the chromosomes, except for pair 5 and X2, while the U2 snDNA was placed exclusively in the largest chromosome. Finally, the 5S rDNA was exclusively located in the short arms of the sex chromosomes. The obtained data reinforce the importance of chromosomal dissociation and inversion as a primary evolutionary mechanism to generate neo-sex chromosomes in the species studied, followed by the repetitive DNAs accumulation. Moreover the exclusive placement of 5S rDNA in the sex chromosomes suggests the involvement of this sequence in sex chromosome recognition throughout meiosis and, consequently, their maintenance, in addition to their avoiding degeneration.

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“…These regions can be hotspots for the accumulation of SSRs and might be associated with the structural formation of the telomere and participate in chromosome rearrangements as proposed for Semaprochilodus [Terencio et al, 2013]. The same subterminal pattern was seen for (CG) 15 and (TA) 15 probes, but only in C. gomesi.…”

Section: Localization Of Microsatellites In the C Zebra And C Gomessupporting

confidence: 55%

Chromosomal Spreading of Microsatellites and (TTAGGG)n Sequences in the Characidium zebra and C. gomesi Genomes (Characiformes: Crenuchidae)

Pucci

Barbosa

Nogaroto

et al. 2016

Cytogenet Genome Res

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Sex chromosome evolution involves the accumulation of repeat sequences such as multigenic families, noncoding repetitive DNA (satellite, minisatellite, and microsatellite), and mobile elements such as transposons and retrotransposons. Most species of Characidium exhibit heteromorphic ZZ/ZW sex chromosomes; the W is characterized by an intense accumulation of repetitive DNA including dispersed satellite DNA sequences and transposable elements. The aim of this study was to analyze the distribution pattern of 18 different tandem repeats, including (GATA)_n and (TTAGGG)_n, in the genomes of C. zebra and C. gomesi, especially in the C. gomesi W chromosome. In the C. gomesi W chromosome, weak signals were seen for (CAA)₁₀, (CAC)₁₀, (CAT)₁₀, (CGG)₁₀, (GAC)₁₀, and (CA)₁₅ probes. (GA)₁₅ and (TA)₁₅ hybridized to the autosomes but not to the W chromosome. The (GATA)_n probe hybridized to the short arms of the W chromosome as well as the (CG)₁₅ probe. The (GATA)_n repeat is known to be a protein-binding motif. GATA-binding proteins are necessary for the decondensation of heterochromatic regions that hold coding genes, especially in some heteromorphic sex chromosomes that may keep genes related to oocyte development. The (TAA)₁₀ repeat is accumulated in the entire W chromosome, and this microsatellite accumulation is probably involved in the sex chromosome differentiation process and crossover suppression in C. gomesi. These additional data on the W chromosome DNA composition help to explain the evolution of sex chromosomes in Characidium.

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Evolutionary Dynamics of Repetitive DNA in <b><i>Semaprochilodus </i></b>(Characiformes, Prochilodontidae): A Fish Model for Sex Chromosome Differentiation

Cited by 25 publications

References 38 publications

Chromosomal Mapping of Repetitive DNAs in <b><i>Characidium</i></b> (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes

Chromosomal Mapping of Repetitive DNAs in <b><i>Characidium</i></b> (Teleostei, Characiformes): Genomic Organization and Diversification of ZW Sex Chromosomes

Repetitive DNA chromosomal organization in the cricket Cycloptiloides americanus: a case of the unusual X1X20 sex chromosome system in Orthoptera

Chromosomal Spreading of Microsatellites and (TTAGGG)<sub>n</sub> Sequences in the <b><i>Characidium zebra </i></b>and<b><i> C. gomesi </i></b>Genomes (Characiformes: Crenuchidae)

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