Evolutionary Engineering Improves Tolerance for Replacement Jet Fuels in Saccharomyces cerevisiae

Brennan, Timothy C. R.; Williams, Thomas; Schulz, Benjamin L.; Palfreyman, Robin W.; Krömer, Jens O.; Nielsen, Lars K.

doi:10.1128/aem.04144-14

Cited by 52 publications

(36 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tolerance of S. cerevisiae toward limonene and other monoterpenes could be enhanced by expression of a fungal efflux pump that was demonstrated to be involved in monoterpene resistance of the bark beetle-associated pine tree pathogen Grosmannia clavigera (Wang et al 2013 ). Recently, evolutionary engineering of S. cerevisiae demonstrated a truncation of a tricalbin protein with a probable function in cell wall integrity regulation to confer a drastic improvement in tolerance toward limonene and other monoterpenes (Brennan et al 2015 ).…”

Section: Toxicity and Solvent Tolerancementioning

confidence: 99%

Biotechnological production of limonene in microorganisms

Jongedijk

Cankar²,

Buchhaupt

et al. 2016

Appl Microbiol Biotechnol

150

103

View full text Add to dashboard Cite

This mini review describes novel, biotechnology-based, ways of producing the monoterpene limonene. Limonene is applied in relatively highly priced products, such as fragrances, and also has applications with lower value but large production volume, such as biomaterials. Limonene is currently produced as a side product from the citrus juice industry, but the availability and quality are fluctuating and may be insufficient for novel bulk applications. Therefore, complementary microbial production of limonene would be interesting. Since limonene can be derivatized to high-value compounds, microbial platforms also have a great potential beyond just producing limonene. In this review, we discuss the ins and outs of microbial limonene production in comparison with plant-based and chemical production. Achievements and specific challenges for microbial production of limonene are discussed, especially in the light of bulk applications such as biomaterials.Electronic supplementary materialThe online version of this article (doi:10.1007/s00253-016-7337-7) contains supplementary material, which is available to authorized users.

show abstract

Section: Toxicity and Solvent Tolerancementioning

confidence: 99%

Biotechnological production of limonene in microorganisms

Jongedijk

Cankar²,

Buchhaupt

et al. 2016

Appl Microbiol Biotechnol

150

103

View full text Add to dashboard Cite

show abstract

“…Adaptive evolution has been proven to be effective in improving cellular tolerance against disadvantageous environments and the foundation is beneficial mutant genes [24]. Brennan et al [2] reported that Saccharomyces cerevisiae had a 4-fold increased tolerance toward the biojet fuel blend AMJ-700t because of one gene mutant (tTcb3p). The mutant E72 from Bacillus subtlis168 exhibited higher growth rate and xylose consumption rate with three mutations (araR, sinR, and comP) [32].…”

Section: Discussionmentioning

confidence: 99%

Enhanced Acid Tolerance in Bifidobacterium longum by Adaptive Evolution: Comparison of the Genes between the Acid-Resistant Variant and Wild-Type Strain

Jiang

Ren

Liu

et al. 2016

Journal of Microbiology and Biotechnology

View full text Add to dashboard Cite

Acid stress can affect the viability of probiotics, especially Bifidobacterium. This study aimed to improve the acid tolerance of Bifidobacterium longum BBMN68 using adaptive evolution. The stress response, and genomic differences of the parental strain and the variant strain were compared by acid stress. The highest acid-resistant mutant strain (BBMN68m) was isolated from more than 100 asexual lines, which were adaptive to the acid stress for 10(th), 20(th), 30(th), 40(th), and 50(th) repeats, respectively. The variant strain showed a significant increase in acid tolerance under conditions of pH 2.5 for 2 h (from 7.92 to 4.44 log CFU/ml) compared with the wildtype strain (WT, from 7.87 to 0 log CFU/ml). The surface of the variant strain was also smoother. Comparative whole-genome analysis showed that the galactosyl transferase D gene (cpsD, bbmn68_1012), a key gene involved in exopolysaccharide (EPS) synthesis, was altered by two nucleotides in the mutant, causing alteration in amino acids, pI (from 8.94 to 9.19), and predicted protein structure. Meanwhile, cpsD expression and EPS production were also reduced in the variant strain (p < 0.05) compared with WT, and the exogenous WT-EPS in the variant strain reduced its acid-resistant ability. These results suggested EPS was related to acid responses of BBMN68.

show abstract

“…Adaptive laboratory evolution was served as a genome-wide method for improving desirable phenotypes without the knowledge of genetic determinants for network information about those phenotypes [22][23]. And it was successfully employed to identify biological solutions to biofuel and alcohol toxicity in S. cerevisiae [24][25][26][27][28][29][30]. In this study, we used adaptive laboratory evolution to enhance yeast strain's tolerance toward higher glucose and isobutanol concentration in fermentation.…”

Section: Introductionmentioning

confidence: 99%

Improving isobutanol productivity through adaptive laboratory evolution in Saccharomyces cerevisiae

Zhang

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: Isobutanol is an ideal second-generation biofuels due to its lower hygroscopicity, higher energy density and higher-octane value. However, isobutanol is toxic to production organisms. To improve isobutanol productivity, adaptive laboratory evolution method was carried out to improve the tolerance of Saccharomyces cerevisiae toward higher isobutanol and higher glucose concentration.Results: We evolved the laboratory strain of S. cerevisiae W303-1A by using EMS (ethyl methanesulfonate) mutagenesis followed by adaptive laboratory evolution. The evolved strain EMS39 with significant increase in growth rate and viability in media with higher isobutanol and higher glucose concentration was obtained. Then, metabolic engineering of the evolved strain EMS39 as a platform for isobutanol production were carried out. Delta integration method was used to over-express ILV3 gene and 2μ plasmids carrying ILV2, ILV5 and ARO10 were used to over-express ILV2, ILV5 and ARO10 genes in the evolved strain EMS39 and wild type W303-1A. And the resulting strains was designated as strain EMS39V2δV3V5A10 and strain W303-1AV2δV3V5A10, respectively. Our results shown that isobutanol titers of the evolved strain EMS39 increased by 30% compared to the control strain. And isobutanol productivity of strain EMS39V2δV3V5A10 increased by 32.4% compared to strain W303-1AV2δV3V5A10. Whole genome resequencing and analysis of site-directed mutagenesis of the evolved strain EMS39 have identified important mutations. In addition, RNA-Seq-based transcriptomic analysis revealed cellular transcription profile changes resulting from EMS39.Conclusions: With the aim of increase productivity of isobutanol in S. cerevisiae, improving tolerance toward higher isobutanol and higher glucose concentration via EMS mutagenesis followed by adaptive evolutionary engineering was conducted. An evolved strain EMS39 with significant increase in growth rate and viability had been obtained. And metabolic engineering of the evolved strain as a platform for isobutanol production was carried out. Furthermore, analysis of whole genome resequencing and transcriptome sequencing were also carried out.

show abstract

Evolutionary Engineering Improves Tolerance for Replacement Jet Fuels in Saccharomyces cerevisiae

Cited by 52 publications

References 40 publications

Biotechnological production of limonene in microorganisms

Biotechnological production of limonene in microorganisms

Enhanced Acid Tolerance in Bifidobacterium longum by Adaptive Evolution: Comparison of the Genes between the Acid-Resistant Variant and Wild-Type Strain

Improving isobutanol productivity through adaptive laboratory evolution in Saccharomyces cerevisiae

Contact Info

Product

Resources

About