Evolutionary origins and sequence of the<i>Escherichia coli</i>O4 O-antigen gene cluster

D'Souza, Jocelyne M.; Samuel, Gabrielle; Reeves, Peter R.

doi:10.1016/j.femsle.2005.01.012

Cited by 12 publications

(8 citation statements)

References 24 publications

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“…Studies mostly based on Salmonella enterica and E. coli (D’Souza et al, 2005; Hu et al, 2010; Reeves et al, 2013) have previously suggested that OAg biosynthetic operons in bacterial pathogens were acquired by horizontal genetic transfer from species with a low GC content. Moreover, horizontal exchange of O-specific antigen biosynthetic genes among phylogenetically distinct P. aeruginosa strains was observed and serotype switching was found to be the result of horizontal transfer and genetic recombination of LPS biosynthetic genes originating from an multidrug-resistant taxonomic outlier P. aeruginosa strain (Thrane et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient

et al. 2017

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Burkholderia cenocepacia is an opportunistic pathogen associated with chronic lung infections and increased risk of death in patients with cystic fibrosis (CF). In this work, we investigated the lipopolysaccharide (LPS) of clinical variants of B. cenocepacia that were collected from a CF patient over a period of 3.5 years, from the onset of infection until death by necrotizing pneumonia (cepacia syndrome). We report the chemical structure of the LPS molecule of various sequential isolates and the identification of a novel hybrid O-antigen (OAg) biosynthetic cluster. The OAg repeating unit of the LPS from IST439, the initial isolate, is a [→2)-β-D-Ribf-(1→4)-α-D-GalpNAc-(1→] disaccharide, which was not previously described in B. cenocepacia. The IST439 OAg biosynthetic gene cluster contains 7 of 23 genes that are closely homologous to genes found in B. multivorans, another member of the Burkholderia cepacia complex. None of the subsequent isolates expressed OAg. Genomic sequencing of these isolates enabled the identification of mutations within the OAg cluster, but none of these mutations could be associated with the loss of OAg. This study provides support to the notion that OAg LPS modifications are an important factor in the adaptation of B. cenocepacia to chronic infection and that the heterogeneity of OAgs relates to variation within the OAg gene cluster, indicating that the gene cluster might have been assembled through multiple horizontal transmission events.

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Section: Discussionmentioning

confidence: 99%

Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient

et al. 2017

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“…The G + C content within the O antigen clusters also greatly differs from gene to gene, indicating that the gene clusters might have been assembled from multiple horizontal transmission events and from several sources over a much longer time (Lerouge and Vanderleyden 2002). The role of lateral gene transfer in the evolution of O antigen clusters and O antigen diversification has been well described in Salmonella (Perepelov et al 2011;Reeves et al 2013), Escherichia (D'Souza, Samuel andReeves 2005;Hu et al 2010;Azmuda et al 2012), Vibrio (González-Fraga et al 2008Wildschutte et al 2010), Yersinia (Cunneen and Reeves 2007) and Brucella (Wattam et al 2014). Another mechanism of variation involves large chromosomal rearrangements.…”

Section: Lateral Gene Transfer Recombination and Genetic Rearrangementsmentioning

confidence: 97%

Lipopolysaccharide modification in Gram-negative bacteria during chronic infection

Maldonado

Sá‐Correia

Valvano

2016

FEMS Microbiology Reviews

508

357

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The Gram-negative bacterial lipopolysaccharide (LPS) is a major component of the outer membrane that plays a key role in host-pathogen interactions with the innate immune system. During infection, bacteria are exposed to a host environment that is typically dominated by inflammatory cells and soluble factors, including antibiotics, which provide cues about regulation of gene expression. Bacterial adaptive changes including modulation of LPS synthesis and structure are a conserved theme in infections, irrespective of the type or bacteria or the site of infection. In general, these changes result in immune system evasion, persisting inflammation and increased antimicrobial resistance. Here, we review the modifications of LPS structure and biosynthetic pathways that occur upon adaptation of model opportunistic pathogens (Pseudomonas aeruginosa, Burkholderia cepacia complex bacteria, Helicobacter pylori and Salmonella enterica) to chronic infection in respiratory and gastrointestinal sites. We also discuss the molecular mechanisms of these variations and their role in the host-pathogen interaction.

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“…The O-antigen gene clusters for many O groups have now been sequenced (Marolda and Valvano, 1993; Bastin and Reeves, 1995; Amor and Whitfield, 1997; Wang and Reeves, 1998; Wang L et al , 1998, 2001, 2002, 2007, 2009, 2010, Paton and Paton, 1999; Shepherd et al , 2000; D'Souza et al , 2002, 2005; Grozdanov et al , 2002; Perelle, et al , 2002; Shao et al , 2003; Fratamico et al , 2003, 2005, 2009a, b, 2010; Feng et al , 2004a, b, 2005a, b, 2007; Guo et al , 2004, 2005; Beutin et al , 2005a, b, 2007; DebRoy et al , 2005; Cheng et al , 2006, 2007; Cunneen and Reeves, 2007; Han et al , 2007; Liu Y et al , 2007, 2008; Liu B et al , 2010; Ren et al , 2008; Perepelov et al , 2009, 2011a, b; Wang Q et al ., 2009, 2010a, b; Li et al , 2010a, b). DNA sequences for about 95 O-antigen gene clusters have been deposited in GenBank so far, and the accession numbers are listed in Table 1.…”

Section: O Antigen Detectionmentioning

confidence: 99%

Detection of O antigens inEscherichia coli

DebRoy

Roberts

Fratamico

2011

Anim. Health. Res. Rev.

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Lipopolysaccharide on the surface of Escherichia coli constitutes the O antigens which are important virulence factors that are targets of both the innate and adaptive immune systems and play a major role in host-pathogen interactions. O antigens are responsible for antigenic specificity of the strain and determine the O serogroup. The designation of O serogroups is important for classifying E. coli strains, for epidemiological studies, in tracing the source of outbreaks of gastrointestinal or other illness, and for linking the source to the infection. For conventional serogroup identification, serotyping by agglutination reactions against antisera developed for each of the O serogroups has been used. In the last decade, many O-antigen gene clusters that encode for the enzymes responsible for the synthesis of the variable oligosaccharide region on the surface of the bacteria have been sequenced and characterized. Unique gene sequences within the O-antigen gene clusters have been targeted for identification and detection of many O groups using the polymerase chain reaction and microarrays. This review summarizes current knowledge on the DNA sequences of the O-antigen gene clusters, genetic-based methods for O-group determination and detection of pathogenic E. coli based on O-antigen and virulence gene detection, and provides perspectives on future developments in the field.

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Evolutionary origins and sequence of theEscherichia coliO4 O-antigen gene cluster

Cited by 12 publications

References 24 publications

Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient

Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient

Lipopolysaccharide modification in Gram-negative bacteria during chronic infection

Detection of O antigens inEscherichia coli

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