2019
DOI: 10.7554/elife.47612
|View full text |Cite
|
Sign up to set email alerts
|

Evolutionary pathways to antibiotic resistance are dependent upon environmental structure and bacterial lifestyle

Abstract: Bacterial populations vary in their stress tolerance and population structure depending upon whether growth occurs in well-mixed or structured environments. We hypothesized that evolution in biofilms would generate greater genetic diversity than well-mixed environments and lead to different pathways of antibiotic resistance. We used experimental evolution and whole genome sequencing to test how the biofilm lifestyle influenced the rate, genetic mechanisms, and pleiotropic effects of resistance to ciprofloxacin… Show more

Help me understand this report
View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

5
116
1

Year Published

2019
2019
2022
2022

Publication Types

Select...
5
2
2

Relationship

0
9

Authors

Journals

citations
Cited by 142 publications
(122 citation statements)
references
References 112 publications
(159 reference statements)
5
116
1
Order By: Relevance
“…The amount of antibiotics bound by LCLB56_AgNPs (1), sorption effectiveness at each incubation period (2) and binding rate constants for zero (3), pseudo-first (4) and -second (5) order kinetic models as well as Weber-Morris intra-particle diffusion model (6) were calculated as it was described by Buszewski et al [12], applying the following equations:…”
Section: Kinetics Study Of Antibiotics Sorption Onto Lclb56 Silver (Bmentioning
confidence: 99%
See 1 more Smart Citation
“…The amount of antibiotics bound by LCLB56_AgNPs (1), sorption effectiveness at each incubation period (2) and binding rate constants for zero (3), pseudo-first (4) and -second (5) order kinetic models as well as Weber-Morris intra-particle diffusion model (6) were calculated as it was described by Buszewski et al [12], applying the following equations:…”
Section: Kinetics Study Of Antibiotics Sorption Onto Lclb56 Silver (Bmentioning
confidence: 99%
“…Nowadays, over 70% of bacterial infections are caused by strains resistant to at least one type of the most commonly used drugs [1]. The mechanism of microbial resistance is very complex, strain specific and can be influenced by many factors, such as growth conditions, life form or the metabolic state of the cells [2][3][4][5]. Therefore, the development of new substances with bactericidal properties is extremely important for health care [1,2,6,7].…”
Section: Introductionmentioning
confidence: 99%
“…The phenomenon of CS has now been described for a variety of bacterial taxa, such as Acinetobacter baumannii, Eschericia coli, Enterococcus faecalis, P. aeruginosa, or Staphylococcus aureus. It's strength and prevalence might depend however on the drug sequence, the bacterial species, and the evolutionary lineage within a bacterial species (44,(49)(50)(51)(52)(53)(54)(55)(56). Overall, CS shows high potential for precision medicine to tailor treatments specific to a pathogen or even a specific strain (49,57).…”
Section: Sequential Treatmentsmentioning
confidence: 99%
“…Biofilms pose a major medical challenge as they can be over 1000-fold more tolerant to antibiotics than their planktonic counterparts [4]. Spatially heterogeneous natural selection in biofilm milieu also contributes to antibiotic resistance development and transfer [5], [6] as well as producing antibiotic-resistant bacteria that are more fit and not easily outcompeted in the absence of the drug [7].…”
Section: Introductionmentioning
confidence: 99%