Evolutionary Relationships of Trichostrongyloid Nematodes (Strongylida) Inferred from Ribosomal DNA Sequence Data

Chilton, Neil B.; Newton, Lisa A.; Beveridge, Ian; Gasser, Robin B.

doi:10.1006/mpev.2001.0938

Cited by 50 publications

(24 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For this reason, specimens were molecularly identified as Haemonchus contortus. Although alternative methods of molecular identification are possible (Blouin, 1997;Bensch et al, 2004), the ITS-2 region for Haemonchus species identification was successfully used by many authors (Chilton et al, 2001;Troell et al, 2003;Brasil et al, 2012) in analysing specimens from various parts of the world.…”

Section: Discussionmentioning

confidence: 99%

Comparative morphological and molecular identification of Haemonchus species in sheep

et al. 2014

View full text Add to dashboard Cite

A combined approach in the determination of Haemonchus nematodes from sheep was applied in this trial. Using selected morphological characters 90.2 % females and 84.2 % males of Haemonchus contortus and 9.8 % females and 15.8 % males of Haemonchus placei were identified. Although cluster analysis based on morphological identification clearly separated two Haemonchus species, H. contortus was exclusively detected in all specimens using restriction cleavage of the ITS-2 region with FspBI endonuclease as well as through the sequencing analysis. Because sheep from both farms have never had contact with other ruminants, and the farmers apply only closed flock turnover, we assume that only H. contortus mono-infection occurred on both farms. This opinion is also supported by molecular data. The most striking result of our study was the finding which indicates that the discriminant function is not able to accurately identify Haemonchus males at the species level.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative morphological and molecular identification of Haemonchus species in sheep

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The sequence pool for this region will undoubtedly continue to grow, due to its frequent application in phylogenetic studies of selected taxa (e.g. Chilton et al 2001;Subbotin et al 2001), in studies of potential hybridizations (Hugall et al 1999) or in analyses of intraspecific diversity (Kaplan et al 2000;Elbadri et al 2002;Ye et al 2004). (iv) No phylum-wide primers are available for the mitochondrial cytochrome oxidase I gene in nematodes, in our experience PCR success rates are well below 50% across various taxa within the phylum.…”

Section: Discussion (A) Are Multifocal Images Useful For Barcoding Stmentioning

confidence: 99%

An integrated approach to fast and informative morphological vouchering of nematodes for applications in molecular barcoding

Ley

Morris

et al. 2005

Phil. Trans. R. Soc. B

211

137

View full text Add to dashboard Cite

Molecular surveys of meiofaunal diversity face some interesting methodological challenges when it comes to interstitial nematodes from soils and sediments. Morphology-based surveys are greatly limited in processing speed, while barcoding approaches for nematodes are hampered by difficulties of matching sequence data with traditional taxonomy. Intermediate technology is needed to bridge the gap between both approaches. An example of such technology is video capture and editing microscopy, which consists of the recording of taxonomically informative multifocal series of microscopy images as digital video clips. The integration of multifocal imaging with sequence analysis of the D2D3 region of large subunit (LSU) rDNA is illustrated here in the context of a combined morphological and barcode sequencing survey of marine nematodes from Baja California and California. The resulting video clips and sequence data are made available online in the database NemATOL (http://nematol.unh.edu/ ). Analyses of 37 barcoded nematodes suggest that these represent at least 32 species, none of which matches available D2D3 sequences in public databases. The recorded multifocal vouchers allowed us to identify most specimens to genus, and will be used to match specimens with subsequent species identifications and descriptions of preserved specimens. Like molecular barcodes, multifocal voucher archives are part of a wider effort at structuring and changing the process of biodiversity discovery. We argue that data-rich surveys and phylogenetic tools for analysis of barcode sequences are an essential component of the exploration of phyla with a high fraction of undiscovered species. Our methods are also directly applicable to other meiofauna such as for example gastrotrichs and tardigrades.

show abstract

“…The putative sister group for Haemonchus, namely, species of Boehmiella Gebauer, 1932 constituted the sole Haemonchine out-group. Results of analyses by Chilton et al (2001), because of their substantial divergence from the 3 phylogenetic studies cited above, were not used as a basis for out-group comparisons in the current study. In addition, specimens of related Haemonchinae were also examined in a comparative context (Table I).…”

Section: Methodsmentioning

confidence: 99%

“…In contrast, Gouÿ de Bellocq et al (2001) found the ''ostertagiines'' to be a paraphyletic assemblage basal to a monophyletic Haemonchinae as determined in analyses of partial sequences from 28S ribosomal DNA (rDNA) for a limited number of exemplar species as representatives of generic level taxa. Alternatively, results by Chilton et al (2001) based on limited comparisons of the ITS-2 region of rDNA among 21 species of trichostrongyloids were highly divergent with respect to these other studies; haemonchines (limited to H. contortus ϩ H. placei) were considered as the putative sister group of the Cooperiinae or as basal trichostrongyles and consistent bootstrap support was lacking.…”

mentioning

confidence: 88%

Phylogeny for Species of Haemonchus (Nematoda: Trichostrongyloidea): Considerations of Their Evolutionary History and Global Biogeography Among Camelidae and Pecora (Artiodactyla)

Hoberg¹,

Lichtenfels²,

Gibbons³

2004

Journal of Parasitology

View full text Add to dashboard Cite

Evolutionary Relationships of Trichostrongyloid Nematodes (Strongylida) Inferred from Ribosomal DNA Sequence Data

Cited by 50 publications

References 51 publications

Comparative morphological and molecular identification of Haemonchus species in sheep

Comparative morphological and molecular identification of Haemonchus species in sheep

An integrated approach to fast and informative morphological vouchering of nematodes for applications in molecular barcoding

Phylogeny for Species of Haemonchus (Nematoda: Trichostrongyloidea): Considerations of Their Evolutionary History and Global Biogeography Among Camelidae and Pecora (Artiodactyla)

Contact Info

Product

Resources

About