Evolutionary relationships within the Phytophthora cactorum species complex in Europe

Pánek, Matěj; Fér, Tomáš; Mráček, Jaroslav; Tomšovský, Michal

doi:10.1016/j.funbio.2016.03.006

Cited by 27 publications

(33 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the number of described Phytophthora species increasing, recent studies have raised an important concern in the accurate detection of species boundaries using phylogenetic data ( Jung & Burgess 2009 , Pánek et al . 2016 , Safaiefarahani et al .…”

Section: Discussionmentioning

confidence: 99%

An expanded phylogeny for the genus Phytophthora

2017

View full text Add to dashboard Cite

A comprehensive phylogeny representing 142 described and 43 provisionally named Phytophthora species is reported here for this rapidly expanding genus. This phylogeny features signature sequences of 114 ex-types and numerous authentic isolates that were designated as representative isolates by the originators of the respective species. Multiple new subclades were assigned in clades 2, 6, 7, and 9. A single species P. lilii was placed basal to clades 1 to 5, and 7. Phytophthora stricta was placed basal to other clade 8 species, P. asparagi to clade 6 and P. intercalaris to clade 10. On the basis of this phylogeny and ancestral state reconstructions, new hypotheses were proposed for the evolutionary history of sporangial papillation of Phytophthora species. Non-papillate ancestral Phytophthora species were inferred to evolve through separate evolutionary paths to either papillate or semi-papillate species.

show abstract

Section: Discussionmentioning

confidence: 99%

An expanded phylogeny for the genus Phytophthora

2017

View full text Add to dashboard Cite

show abstract

“…We also identified P. × serendipita, a natural hybrid of P. cactorum and P. hedraiandra, that has been described on a range of horticultural hosts (Man in 't Veld et al, 2007). Pánek et al (2016), who also included several Swiss isolates previously identified as P. cactorum or P. hedraiandra, suggested that the P. cactorum species complex was an assemblage of closely related lineages that occasionally hybridize. Hence, we assume that P. × serendipita is more widely distributed in natural environments than the single identification in this study would suggest.…”

Section: Discussionmentioning

confidence: 63%

“…In this work, we identified several isolates as P. × cambivora and associated their genotypes with recently described hybrid genotypes . Pánek et al (2016), who also included several Swiss isolates previously identified as P. cactorum or P. hedraiandra, suggested that the P. cactorum species complex was an assemblage of closely related lineages that occasionally hybridize. We also identified P. × serendipita, a natural hybrid of P. cactorum and P. hedraiandra, that has been described on a range of horticultural hosts (Man in 't Veld et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…P. × serendipita is a natural hybrid between P. cactorum and P. hedraindra (Man in 't Veld, de Cock, & Summerbell, 2007;Man in 't Veld, Rosendahl, & Hong, 2012). The ITS region of P. × serendipita displays four characteristic double bases at positions 74, 100, 101 and 686 (Man in 't Veld et al, 2007;Pánek, Fér, Mráček, & Tomšovský, 2016). The sequence obtained from a single lesion matched precisely at those positions, and thus the isolate was assigned to P. × serendipita.…”

Section: Presence Of Hybrid Phytophthora Speciesmentioning

confidence: 93%

See 1 more Smart Citation

Multispecies Phytophthora disease patterns in declining beech stands

2019

View full text Add to dashboard Cite

Decline diseases are typically caused by complex abiotic and biotic interactions and characterized by a suite of symptoms indicative of low plant vigour. Diseased trees are frequently infected by Phytophthora, but the complex interactions between pathogen, host and the heterogeneous forest environment mask a comprehensive understanding of the aetiology. In the present study, we surveyed European beech (Fagus sylvatica) stands in Swiss forests with recent increases in bleeding lesions for the presence of Phytophthora. We used a combined approach of analysing soil and bark samples from trees displaying bleeding lesions and trees free from bleeding lesions. Soil baiting revealed a higher prevalence of Phytophthora spp. around trees with bleeding lesions than around trees without bleeding lesions. For the bark samples from bleeding lesions, we used several detection methods. Phytophthora spp. were detected in 74% of the trees by an immunological on‐site diagnostic kit, in 64% by a specific PCR assay, and 38% by isolation on selective media. All samples tested were negative for P. ramorum using qPCR. Overall, nine Phytophthora species were identified by ITS sequencing, the most common of which were P. plurivora, P. gonapodyides, P. × cambivora and P. syringae. We identified distinct species in bleeding lesions and the rhizosphere of the same host tree which suggests a multispecies Phytophthora disease patterns in these declining beech. Among the recovered species, P. × cambivora and P. × serendipita were identified as hybrid genotypes with the former abundant in bleeding lesions.

show abstract

“…Furthermore, intraspecific variation and interweaving characters make it a difficult task for correct identification of isolates, even for experts and specialists. Moreover, some species like Phytophthora megasperma , Phytophthora cryptogea probably consist of species complexes rather than a single taxa (Bonants et al ; Safaiefarahani et al ; Panek et al ).…”

Section: Introductionmentioning

confidence: 99%

SCAR marker forPhytophthora nicotianaeand a multiplex PCR assay for simultaneous detection ofP. nicotianaeandCandidatusLiberibacter asiaticus in citrus

et al. 2019

View full text Add to dashboard Cite

Aims This study aimed to develop a random amplified polymorphic DNA (RAPD)‐based sequence characterized amplified region (SCAR) marker for species‐specific detection of Phytophthora nicotianae, a global plant pathogen. Another objective was to develop a multiplex PCR assay for simultaneous detection of P. nicotianae and huanglongbing‐causing bacterium, Candidatus Liberibacter asiaticus (CaLas) in citrus roots using the developed SCAR marker and a previously published 16SrDNA‐based CaLas‐specific primer set. Methods and Results The RAPD primer, OPA4, amplified a specific fragment of c. 400 bp only in P. nicotianae isolates. The fragment was eluted, purified, cloned and sequenced. One set of SCAR primers (SCAR4F/SCAR4R1), developed from the sequence information of the fragment, was found specific to P. nicotianae and produced an amplicon of 330 bp size, and was found non‐specific to the five Phytophthora species (P. citrophthora, P. palmivora, P. lacustris, P. boehmeriae and P. insolita) and five other pathogens (Mycosphaerella citri, Alternaria alternata, Septobasidium pseudopedicillatum, Phytopythium vexans and Colletotrichum gloeosporioides) isolated from the citrus agroecosystem. The sensitivity of the primer pair was 5 pg µl−1 of mycelial DNA. Furthermore, the specific SCAR primers coupled with a previously reported CaLas‐specific primer set were used effectively in developing a multiplex PCR assay to detect P. nicotianae and CaLas simultaneously in root tissues of citrus plants. Conclusions A rapid method using a RAPD‐based SCAR marker for the detection of P. nicotianae was developed. Furthermore, a multiplex PCR assay was established for simultaneous detection of P. nicotianae and CaLas in citrus roots. Significance and Impact of the Study A RAPD‐SCAR marker‐based detection system and the one‐step multiplex PCR method developed in this study can be applied to index citrus trees infected (individually or conjointly) with P. nicotianae and CaLas. The present technique developed would also be useful in monitoring disease epidemiology and phytosanitary surveillance.

show abstract

Evolutionary relationships within the Phytophthora cactorum species complex in Europe

Cited by 27 publications

References 58 publications

An expanded phylogeny for the genus Phytophthora

An expanded phylogeny for the genus Phytophthora

Multispecies Phytophthora disease patterns in declining beech stands

SCAR marker forPhytophthora nicotianaeand a multiplex PCR assay for simultaneous detection ofP. nicotianaeandCandidatusLiberibacter asiaticus in citrus

Contact Info

Product

Resources

About