Ex situ reproduction and recruitment of scleractinian coral Galaxea fascicularis

Fang, Wei; Cui, Mengyao; Huang, Wen; Wang, Yonggang; Liu, Xu; Zeng, Xinru; Su, Hongfei; Yu, Kefu

doi:10.1007/s00227-023-04175-7

Cited by 5 publications

(1 citation statement)

References 46 publications

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“…Historically, working with coral gametes has been difficult due to them only spawning once a year. With developments in ex-situ spawning, there is now higher availability of coral gametes throughout the year [53][54][55] making gamete based research easier and more accessible for coral species. This, paired with new karyotyping methodologies for non-model invertebrate organisms [56], will allow experimental identification of coral species karyotype to occur, paving the way for improved genome assemblies due to known chromosome number.…”

Section: Potential Chromosomes Are Recovered From Hifi Reads Without ...mentioning

confidence: 99%

Annotated genome and transcriptome of the endangered Caribbean mountainous star coral (Orbicella faveolata) using PacBio long-read sequencing

Young,

Williamson,

Kron

et al. 2024

BMC Genomics

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Long-read sequencing is revolutionizing de-novo genome assemblies, with continued advancements making it more readily available for previously understudied, non-model organisms. Stony corals are one such example, with long-read de-novo genome assemblies now starting to be publicly available, opening the door for a wide array of ‘omics-based research. Here we present a new de-novo genome assembly for the endangered Caribbean star coral, Orbicella faveolata, using PacBio circular consensus reads. Our genome assembly improved the contiguity (51 versus 1,933 contigs) and complete and single copy BUSCO orthologs (93.6% versus 85.3%, database metazoa_odb10), compared to the currently available reference genome generated using short-read methodologies. Our new de-novo assembled genome also showed comparable quality metrics to other coral long-read genomes. Telomeric repeat analysis identified putative chromosomes in our scaffolded assembly, with these repeats at either one, or both ends, of scaffolded contigs. We identified 32,172 protein coding genes in our assembly through use of long-read RNA sequencing (ISO-seq) of additional O. faveolata fragments exposed to a range of abiotic and biotic treatments, and publicly available short-read RNA-seq data. With anthropogenic influences heavily affecting O. faveolata, as well as its increasing incorporation into reef restoration activities, this updated genome resource can be used for population genomics and other ‘omics analyses to aid in the conservation of this species.

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Section: Potential Chromosomes Are Recovered From Hifi Reads Without ...mentioning

confidence: 99%

Annotated genome and transcriptome of the endangered Caribbean mountainous star coral (Orbicella faveolata) using PacBio long-read sequencing

Young,

Williamson,

Kron

et al. 2024

BMC Genomics

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Lipid profiling during embryogenesis of coral Galaxea fascicularis

Buttari,

Tsai,

Wen

et al. 2024

Mar Biol

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Hypoxia threatens coral and sea anemone early life stages

Glass,

Barott

2024

Preprint

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Seawater hypoxia is increasing globally and can drive declines in organismal performance across a wide range of marine taxa. However, the effects of hypoxia on early life stages (e.g., larvae and juveniles) are largely unknown, and it is unclear how evolutionary and life histories may influence these outcomes. Here, we addressed this question by comparing hypoxia responses across early life stages of three cnidarian species representing a range of life histories: the reef-building coralGalaxea fascicularis, a broadcast spawner with horizontal transmission of endosymbiotic algae (family Symbiodiniaceae); the reef-building coralPorites astreoides, a brooder with vertical endosymbiont transmission; and the estuarine sea anemoneNematostella vectensis, a non-symbiotic broadcast spawner. Transient exposure of larvae to hypoxia (dissolved oxygen < 2 mg L-1for 6 h) led to decreased larval swimming and growth for all three species, which resulted in impaired settlement for the corals. Coral-specific responses also included larval swelling, depressed respiration rates, and decreases in symbiont densities and function. These results indicate both immediate and latent negative effects of hypoxia on cnidarian physiology and coral-algal mutualisms specifically. In addition,G. fascicularisandP. astreoideswere sensitized to heat stress following hypoxia exposure, suggesting that the combinatorial nature of climate stressors will lead to declining performance for corals. However, sensitization to heat stress was not observed inN. vectensisexposed to hypoxia, suggesting that this species may be more resilient to combined stressors. Overall, these results emphasize the importance of reducing anthropogenic carbon emissions to limit further ocean deoxygenation and warming.

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Ex situ reproduction and recruitment of scleractinian coral Galaxea fascicularis

Cited by 5 publications

References 46 publications

Annotated genome and transcriptome of the endangered Caribbean mountainous star coral (Orbicella faveolata) using PacBio long-read sequencing

Annotated genome and transcriptome of the endangered Caribbean mountainous star coral (Orbicella faveolata) using PacBio long-read sequencing

Lipid profiling during embryogenesis of coral Galaxea fascicularis

Hypoxia threatens coral and sea anemone early life stages

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