Ex vitro Morpho-Physiological Screening of Drought Tolerant Sugarcane Epimutants Generated Via 5-Azacytidine and Imidacloprid Treatments

Koetle, M.J.; Snyman, S. J.; Rutherford, R. S.

doi:10.1007/s12042-022-09323-9

Cited by 5 publications

(5 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These studies provided useful insights into the role of epigenetic mechanisms in grapevine defense against downy mildew and their potential implications for future breeding programs such as improving tolerance to powdery mildew in grapevine and reducing the massive current and recurring use of chemicals. Additionally, the use of DNA methyltransferases blockers (including but not limited to 5-azacytidine, 5-aza-2'deoxycytidine, 1-beta-D-arabinofuranosyl-5-azacytosine and dihydro-5-azacytidine) has been proposed as an approach to generate epigenetic variation for crop improvement (Amoah et al, 2012), as recently implemented in the development of drought-tolerant sugarcane epimutants (Koetle et al, 2022).…”

Section: Non-coding Rna-mediated Gene Regulationmentioning

confidence: 99%

Epigenomics: a new tool for the generation of climate resilient grapevines

Tan¹,

López²

2023

Front. Hortic.

View full text Add to dashboard Cite

Climate change is expected to increase the occurrence of extreme environmental conditions. Viticulture, as agriculture in general, is highly dependent on climatic conditions, not only for yield but also for fruit quality, which is the most important factor affecting produce value at the farm-gate. This demands the development of novel plant breeding techniques that will lead to the accelerated production of more resilient grape varieties, as conventional breeding programs for perennials are often prolonged. Recent research has suggested that environmental conditions can activate a memory of stress that could result in a primed response to subsequent stress events. This is a process capable of increasing plant’s resilience to abiotic stimuli, allowing plants to better adapt to extreme environmental conditions. While the effect of priming has been observed in many plants, the underlying mechanisms are puzzling and seldom studied in perennial crops. A large body of research has been developed in the last decade linking response to stress, stress priming, and memory of stress with epigenetic mechanisms. This understanding of plant epigenetics has opened the door to the application of epigenetics to crop improvement, such as the use of epigenetic breeding for the generation of more resilient crops. Perennial crop agriculture in general, and viticulture in particular, would benefit from more in-depth knowledge on epigenetic memory of stress.

show abstract

Section: Non-coding Rna-mediated Gene Regulationmentioning

confidence: 99%

Epigenomics: a new tool for the generation of climate resilient grapevines

Tan¹,

López²

2023

Front. Hortic.

View full text Add to dashboard Cite

show abstract

“…In an experiment comparing ROS formation and antioxidant enzymes in C 3 and C 4 plants, Uzilday et al (2012) proposed that although the induction rate of antioxidant enzymes in C 4 plants was lower, their antioxidant system was more effective at coping with ROS formation under stress conditions. The study by Kandoi et al (2018) greater drought and salt resistance in C 4 plants (Figures 2 and 7b) (Gohil et al, 2022;Koetle et al, 2022;Wang et al, 2022). The L r of C 4 plants decreased more, but the DW p of C 4 plants decreased less than that of C 3 plants under drought and combined stresses, indicating better stress resistance in C 4 plants through the maintenance of aboveground growth (Kumar et al, 2014).…”

Section: Discussionmentioning

confidence: 91%

“…The H p , TLA and N leaf of C 4 plants decreased less than those of C 3 plants under drought, salt, and combined stresses, indicating greater drought and salt resistance in C 4 plants (Figures 2 and 7b) (Gohil et al, 2022; Koetle et al, 2022; Wang et al, 2022). The L r of C 4 plants decreased more, but the DW p of C 4 plants decreased less than that of C 3 plants under drought and combined stresses, indicating better stress resistance in C 4 plants through the maintenance of above‐ground growth (Kumar et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

A meta‐analysis highlights the cross‐resistance of plants to drought and salt stresses from physiological, biochemical, and growth levels

Cao,

Ding,

et al. 2024

Physiologia Plantarum

View full text Add to dashboard Cite

In nature, drought and salt stresses often occur simultaneously and affect plant growth at multiple levels. However, the mechanisms underlying plant responses to drought and salt stresses and their interactions are still not fully understood. We performed a meta‐analysis to compare the effects of drought, salt, and combined stresses on plant physiological, biochemical, morphological and growth traits, analyze the different responses of C3 and C4 plants, as well as halophytes and non‐halophytes, and identify the interactive effects on plants. There were numerous similarities in plant responses to drought, salt, and combined stresses. C4 plants had a more effective antioxidant defense system, and could better maintain above‐ground growth. Halophytes could better maintain photosynthetic rate (Pn) and relative water content (RWC), and reduce growth as an adaptation strategy. The responses of most traits (Pn, RWC, chlorophyll content, soluble sugar content, H2O2 content, plant dry weight, etc.) to combined stress were less‐than‐additive, indicating cross‐resistance rather than cross‐sensitivity of plants to drought and salt stresses. These results are important to improve our understanding of drought and salt cross‐resistance mechanisms and further induce resistance or screen‐resistant varieties under stress combination.

show abstract

“…Por fim, o quinto estudo conduzido por Koetle et al (2022), avaliou a tolerância à seca de dez epimutantes obtidos in vitro através de desmetilação de 5-Azacitidina (Azac) e subsequente remetilação com imidaclopride (IMI), sob estresse osmótico por polietilenoglicol (PEG) e estresse térmico. A aclimatação fora do ambiente de cultivo envolveu quatro ciclos de estresse hídrico repetidos em estufa.…”

Section: Discussionunclassified

“…Perspectivas futuras apontam que a exploração dos mecanismos moleculares da metilação do DNA, abrangendo enzimas de metilação, desmetilação e proteínas de reconhecimento, tais como a 5-Azacitidina mencionados por Koetle et al (2022) e Sammarco et al (2022), permitirá manipulações mais precisas e uma compreensão aprofundada de como a metilação do DNA influencia o desenvolvimento e as respostas das plantas.…”

Section: Conclusãounclassified

Os Avanços Da Epigenética Vegetal Em Tempos De Mudanças Climáticas: Uma Revisão De Literatura

Cruz Paula,

Alves Porto Rodrigues,

Bousquet da Silva Pinto

et al. 2023

Anais Do XXVII INIC, XXIII EPG, XVII INIC Jr, XIII INID, III ENEXUN

View full text Add to dashboard Cite

Os mecanismos epigenéticos conferem às plantas recursos que possibilitam a adaptação a ambientes específicos e, consequentemente, a diferentes estresses, aumentando sua capacidade de sobrevivência. Na biologia das plantas, a metilação do DNA desempenha um papel crucial, influenciando seu desenvolvimento, resposta ao estresse, aclimatação e adaptação. Nessa perspectiva, observa-se que a metilação do DNA pode induzir respostas genéticas distintas em diferentes ambientes. Portanto, esta revisão bibliográfica teve como objetivo investigar os avanços dos mecanismos epigenéticos, com ênfase na metilação do DNA, quando associados às mudanças climáticas. Em conjunto, esses estudos destacaram a importância da epigenética como uma ferramenta fundamental para compreender e aprimorar a adaptação das plantas às transformações climáticas e aos desafios ambientais, oferecendo novas estratégias para o desenvolvimento de culturas mais resilientes e adaptáveis.Palavras-chave: Epigenoma. Metilação do DNA. Alterações climáticas.

show abstract

Ex vitro Morpho-Physiological Screening of Drought Tolerant Sugarcane Epimutants Generated Via 5-Azacytidine and Imidacloprid Treatments

Cited by 5 publications

References 75 publications

Epigenomics: a new tool for the generation of climate resilient grapevines

Epigenomics: a new tool for the generation of climate resilient grapevines

A meta‐analysis highlights the cross‐resistance of plants to drought and salt stresses from physiological, biochemical, and growth levels

Os Avanços Da Epigenética Vegetal Em Tempos De Mudanças Climáticas: Uma Revisão De Literatura

Contact Info

Product

Resources

About