Ex Vitro Rooting of Sugarcane (Saccharum officinarum L.) Plantlets Derived from Tissue Culture

Abstract

A study was conducted at Holetta National Agricultural Biotechnology Laboratory with the objective to determine the effect of different concentrations of NAA on ex vitro root development of sugarcane microshoots. Five levels of NAA (0, 10, 20, 30 and 40 mg/l) and two levels of genotypes were combined in factorial arrangement. The basal end of the shoots was dipped in NAA solution overnight before the shoots were transferred into a plastic tray containing a mixed growing medium in green house. The results showed that interaction effect of genotype and NAA was highly significant (p<0.0001) on rooting percentage, number of roots per shoot, root length. In genotype N52, best root formation was found on the shoots treated with 20 mg/l NAA by which rooting percentage was 76 ± 5.48 with 5.88 ± 0.04 cm root length and 8.06 ± 0.13 number of roots per plantlets. While in genotype N53 maximum, root formation was recorded on the shoots dipped in 30 mg/l NAA by which rooting percentage was 70 ± 7.07 with 5.42 ± 0.11 cm root length and 4.52 ± 0.19 number of roots per plantlets. Shoots rooted through this method exhibited 100 % survival in both genotypes.

Stimulus to rooting of Saccharum sp. mini-cuttings with talc indole-butyric acid

“…However, the use of indole-butyric acid applied in the form of talc for the rooting of sugarcane mini-cuttings has not yet been reported in the literature. Tesfa et al (2016), when studying ex vitro rooting of sugarcane plants derived from micropropagation, observed that the treatment of shoots with solution containing 20 mg L -1 of naphthalene-acetic acid for 24 hours increased rooting by 40% when compared to the control without regulator.…”