Ex-vivo Autofluorescence Measurements of Human Tissues

Delgado, José A.; Anasagasti, L.; Quesada, I.; Cruz, Julia C.; Joan, Alejandro Y.

doi:10.1063/1.1615095

Cited by 1 publication

(3 citation statements)

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“…33 In that study, NADH was extracted from both fresh tissues (processed within 3 days after tissue resection) and frozen tissue (maintained in −20°C for 2 to 4 months). Some spectroscopic studies also showed that NADH intensity in the BC tissue was ∼2 to 3 fold of the normal counterpart as estimated by the fluorescence intensity at ∼460 nm, [34][35][36] although another spectroscopic study showed NADH in the BC tissue was only ∼15% higher than that in the normal ones. 37 Studies looking at fixed tissue slides also found higher NADH fluorescence in the breast tumor region compared to the normal region.…”

Section: Discussionmentioning

confidence: 99%

“…The autofluorescence decay after tissue resection but before being frozen has been a concern for optical studies. 36,[54][55][56] The resected breasts/tissue chunks from which the biopsies were collected had been kept at room temperature for an average of 25 min. Therefore, it is likely that both NADH and Fp contents in these tissues had changed during this time period before collecting tissue aliquots for the redox scanning.…”

Section: Discussionmentioning

confidence: 99%

“…The optical redox ratios [e.g., Fp/NADH or the normalized Fp redox ratio ¼ Fp∕ðNADH þ FpÞ are sensitive to the mitochondrial NAD-coupled redox state and correlate with the NAD þ ∕NADH redox potentials determined by both biochemical assays and liquid chromatography-mass spectrometry. 25,[27][28][29] Although many fluorescence spectroscopic studies of clinical breast samples have reported higher than normal NADH and/or Fp in cancer tissues, [30][31][32][33][34][35][36][37][38] fluorescence imaging of NADH, Fp, and the redox ratios provides spatial and metabolic heterogeneity of tissue, 5,38 which might provide additional valuable information for revealing disease state and discriminating between premalignant and normal tissues. 12,39,40 In recent years, more reports on imaging NADH and Fp fluorescence and the redox ratio of clinical tissue samples have become available.…”

Section: Introductionmentioning

confidence: 99%

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Optical redox imaging indices discriminate human breast cancer from normal tissues

Tchou

Feng

et al. 2016

J. Biomedical Optics

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Our long-term goal was to investigate the potential of incorporating redox imaging technique as a breast cancer (BC) diagnosis component to increase the positive predictive value of suspicious imaging finding and to reduce unnecessary biopsies and overdiagnosis. We previously found that precancer and cancer tissues in animal models displayed abnormal mitochondrial redox state. We also revealed abnormal mitochondrial redox state in cancerous specimens from three BC patients. Here, we extend our study to include biopsies of 16 patients. Tissue aliquots were collected from both apparently normal and cancerous tissues from the affected cancer-bearing breasts shortly after surgical resection. All specimens were snap-frozen and scanned with the Chance redox scanner, i.e., the three-dimensional cryogenic NADH/Fp (reduced nicotinamide adenine dinucleotide/oxidized flavoproteins) fluorescence imager. We found both Fp and NADH in the cancerous tissues roughly tripled that in the normal tissues ( p < 0.05 ). The redox ratio Fp/(NADH + Fp) was ? 27 % higher in the cancerous tissues ( p < 0.05 ). Additionally, Fp, or NADH, or the redox ratio alone could predict cancer with reasonable sensitivity and specificity. Our findings suggest that the optical redox imaging technique can provide parameters independent of clinical factors for discriminating cancer from noncancer breast tissues in human patients.

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