Ex vivo effects of flavonoïds extracted from Artemisia herba alba on cytokines and nitric oxide production in Algerian patients with Adamantiades-Behçet's disease

Abstract

BackgroundAdamantiades-Behçet's disease (ABD) is a chronic multisystemic inflammation with unknown pathophysiology. This disorder is associated with a dysregulation of the cytokine network that hyperactivates neutrophils and macrophages. In this study, we investigate the modulatory effects of flavonoïd compounds extracted from Algerian medicinal plant Artemisia herba alba on Th1 and Th2 cytokines and nitric oxide production.MethodsThe modulatory effects of flavonoïds extracted from Artemisia herba alba on cytokines and nitric oxide production by peripheral blood mononuclear cells isolated from Algerian ABD patients and healthy controls were respectively measured by means of ELISA assays and Griess modified method.ResultsOur results show that flavonoïds significantly reduce the production of interleukin-12, the key effector of T helper 1 (Th1) cells and nitric oxide in a dose-dependent manner in Adamantiades-Behçet's disease. In contrast, the production of IL-4, the key marker of Th2 cells was increased.ConclusionThis study suggests that in vitro supplementation with flavonoïds extracted from Artemisia herba alba could have potential immuno-modulatory effects characterised by a down-regulation and up-regulation of Th1 and Th2 cytokines, respectively. Moreover, flavonoïds may prevent nitric oxide induced damages.

The use of medicinal herbs in gynecological and pregnancy-related disorders by Jordanian women: a review of folkloric practice vs. evidence-based pharmacology

“…Immunomodulatory (Messaoudene et al 2011), antioxidative (Aouadi et al 2014) and insecticidal properties (Abbad et al 2014) along with hypoglycemic activity (Afifi-Yazar et al 2011;Awad et al 2012). Anticancer and inflammatory (Khlifi et al 2013) as well as phytotoxic and antimicrobial activities (Amri et al 2013 (Alzweiri et al 2011).…”

“…: A. inculta) -Asteraceae -Aerial parts and roots decoction for menstrual and nervous problems (Alzweiri et al 2011) Flavonoids (hispidulin and cirsilineol), herbalbin, cischryanthenyl acetate (Afifi-Yazar et al 2011;Messaoudene et al 2011;Moufid & Eddouks 2012); Sesquiterpene lactones (Mohamed et al 2013). …”

Antidiabetic, Antihyperlipidemic and Antioxidant Effects of Artemisia herba alba Extract on Experimental Diabetes

“…Artemisia herba alba was known traditionally to have a variety of biological effects including hypoglycemic, hypolipidemic, antiinflammatory, antifungal and antimicrobial activities (Al-Shamaony et al, 1994;Mohamed et al, 2010;Messaoudene et al, 2011). For the hypolipidemic and antioxidant effects, scientific data on its efficacy are scarce.…”

T Cells Activation and Interferon-γ/Nitric Oxide Production during Behçet Disease: A Study in Algerian Patients

“…2 The involvement of cytokines and chemokines in BD pathogenesis is reflected by the increase of their concentrations in sera and in PBMC of patients with BD and some of these mediators correlate with the clinical activity of the disease. 2,3 The study of CDs expression, as a markers of Tcells in relation with IFNγ production involved in the immune responses during BD appears necessary to situate Th1 immune response in the evolution and may be in the genesis of BD. 4,5 IFNγ is a pivotal Th1 cytokine triggered NO synthase 2induction.…”

“…4,5 IFNγ is a pivotal Th1 cytokine triggered NO synthase 2induction. 2,3 Several works reported its production during the Behçet disease. 3,4 We report here the study of CD3,CD4, CD8 expression in BD Algerian patients in relation with IFNγ and NO production.…”