Ex vivo electroporation as a potent new strategy for nonviral gene transfer into autologous vein grafts

Yamaoka, Terutoshi; Yonemitsu, Yoshikazu; Komori, Kimihiro; Baba, Hironori; Matsumoto, Takuya; Onohara, Toshihiro; Maehara, Yoshihiko

doi:10.1152/ajpheart.00353.2005

Cited by 9 publications

(10 citation statements)

References 43 publications

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“…Because non-viral strategies demonstrate low transduction efficiency and transient transgene expression, viral transfer methods seem to have a greater potential for a successful gene transfer (Yamaoka et al, 2005;Doebis et al, 2006;Boldicke, 2007). Intrabody expression in HeLa cells using the adenoviral system was achieved in this study and resulted in decreasing of CD147 cell surface expression on these transduced cells (Fig.…”

Section: Article In Pressmentioning

confidence: 84%

Potent inhibition of OKT3-induced T cell proliferation and suppression of CD147 cell surface expression in HeLa cells by scFv-M6-1B9

Intasai¹,

Tragoolpua²,

Pingmuang³

et al. 2009

Immunobiology

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Section: Article In Pressmentioning

confidence: 84%

Potent inhibition of OKT3-induced T cell proliferation and suppression of CD147 cell surface expression in HeLa cells by scFv-M6-1B9

Intasai¹,

Tragoolpua²,

Pingmuang³

et al. 2009

Immunobiology

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“…169 EC identification through immunolabeling of von Willebrand factor, for example, is available for baboons, pigs, dogs, rabbits, rats, and mice, but assessment of EC retention in sheep requires alternative methods. 125,168,[170][171][172][173][174] Cell culture techniques have extended beyond EC seeding to develop vascular grafts that are predominantly or wholly tissue-engineered, requiring demonstration of satisfactory mechanical properties in vitro and assessments of remodeling and long-term performance in vivo. Early attempts using molds of SMCs and type I collagen to create tubes subsequently coated with ECs before use required external Dacron mesh support to allow even venous implantation, with remodeling gradually progressing during a 6-month period.…”

Section: New Challenges In the Assessment Of Tissue-engineered Vasculmentioning

confidence: 99%

Animal models for the assessment of novel vascular conduits

Byrom

Bannon

White

et al. 2010

Journal of Vascular Surgery

158

132

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The development of an ideal small-diameter conduit for use in vascular bypass surgery has yet to be achieved. The ongoing innovation in biomaterial design generates novel conduits that require preclinical assessment in vivo, and a number of animal models have been used for this purpose. This article examines the rationale behind animal models used in the assessment of small-diameter vascular conduits encompassing the commonly used species: baboons, sheep, pigs, dogs, rabbits, and rodents. Studies on the comparative hematology for these species relative to humans are summarized, and the hydrodynamic values for common implant locations are also compared. The large- and small-animal models are then explored, highlighting the characteristics of each that determine their relative utility in the assessment of vascular conduits. Where possible, the performance of expanded polytetrafluoroethylene is given in each animal and in each location to allow direct comparisons between species. New challenges in animal modeling are outlined for the assessment of tissue-engineered graft designs. Finally, recommendations are given for the selection of animal models for the assessment of future vascular conduits.

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“…The electric pulse was regulated as follows: voltage, 20 V; pulse-on time, 10 ms; interval time, 990 ms; and number of pulses, 10. These optimized parameters were determined in preliminary studies by measuring luciferase activity 1 day after electroporation of a CMV-driven renilla luciferase construct at various electrode voltages, pulse numbers, and pulse durations, according to the modified protocols of Matsumoto et al(25) and Yamaoka et al(41). In separate experiments, electroporationFig.…”

mentioning

confidence: 99%

Resveratrol attenuates TNF-α-induced activation of coronary arterial endothelial cells: role of NF-κB inhibition

Csiszár¹,

Smith²,

Labinskyy³

et al. 2006

American Journal of Physiology-Heart and Circulatory Physiology

256

183

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Epidemiological studies suggest that Mediterranean diets rich in resveratrol are associated with reduced risk of coronary artery disease. However, the mechanisms by which resveratrol exerts its cardioprotective effects are not completely understood. Because TNF-alpha-induced endothelial activation and vascular inflammation play a critical role in vascular aging and atherogenesis, we evaluated whether resveratrol inhibits TNF-alpha-induced signal transduction in human coronary arterial endothelial cells (HCAECs). We found that TNF-alpha significantly increased adhesiveness of the monocytic THP-1 cells to HCAECs, an effect that could be inhibited by pretreatment with resveratrol and the NF-kappaB inhibitor pyrrolidine dithiocarbamate. Previously, we found that TNF-alpha activates NAD(P)H oxidases, and our recent data showed that TNF-alpha-induced endothelial activation was prevented by the NAD(P)H oxidase inhibitor apocynin or catalase plus SOD. Resveratrol also inhibited H(2)O(2)-induced monocyte adhesiveness. Using a reporter gene assay, we found that, in HCAECs, TNF-alpha significantly increased NF-kappaB activity, which could be inhibited by resveratrol (>50% inhibition at 10(-6) mol/l) and pyrrolidine dithiocarbamate. Resveratrol also inhibited TNF-alpha-induced, NF-kappaB-driven luciferase expression in rat aortas electroporated with the reporter gene construct. In TNF-alpha-treated HCAECs, resveratrol (in the submicromolar range) significantly attenuated expression of NF-kappaB-dependent inflammatory markers inducible nitric oxide synthase, IL-6, bone morphogenetic protein-2, ICAM-1, and VCAM. Thus resveratrol at nutritionally relevant concentrations inhibits TNF-alpha-induced NF-kappaB activation and inflammatory gene expression and attenuates monocyte adhesiveness to HCAECs. We propose that these anti-inflammatory actions of resveratrol are responsible, at least in part, for its cardioprotective effects.

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Ex vivo electroporation as a potent new strategy for nonviral gene transfer into autologous vein grafts

Abstract: Maehara. Ex vivo electroporation as a potent new strategy for nonviral gene transfer into autologous vein grafts.

Cited by 9 publications

References 43 publications

Potent inhibition of OKT3-induced T cell proliferation and suppression of CD147 cell surface expression in HeLa cells by scFv-M6-1B9

Potent inhibition of OKT3-induced T cell proliferation and suppression of CD147 cell surface expression in HeLa cells by scFv-M6-1B9

Animal models for the assessment of novel vascular conduits

Resveratrol attenuates TNF-α-induced activation of coronary arterial endothelial cells: role of NF-κB inhibition

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