Ex vivo induction of cytokine mRNA expression in human blood samples

Härtel, Christoph; Bein, Gregor; Müller‐Steinhardt, Michael; Klüter, Harald

doi:10.1016/s0022-1759(00)00334-3

Cited by 112 publications

(72 citation statements)

References 24 publications

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“…17) In our study the parameter of E max , which represents IL-2 mRNA expression without CsA shows large intra-individual variation as shown in Table 1 (CV; 25 to 73%), indicating that only one point quantification of IL-2 mRNA expression is difficult to evaluate the biological effects of the drug and monitoring of the allogeneic responses. To avoid such shortcomings in previous studies, we determined IC 50 value from the added CsA concentration and IL-2 mRNA expression levels which was calculated from several points.…”

Section: Discussionmentioning

confidence: 59%

Evaluation of Interleukin-2 mRNA in Whole Blood as a Parameter for Monitoring Cyclosporine Pharmacodynamics

Kuzuya

Kobayashi

Katayama

et al. 2009

Biological & Pharmaceutical Bulletin

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Section: Discussionmentioning

confidence: 59%

Evaluation of Interleukin-2 mRNA in Whole Blood as a Parameter for Monitoring Cyclosporine Pharmacodynamics

Kuzuya

Kobayashi

Katayama

et al. 2009

Biological & Pharmaceutical Bulletin

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“…14,15 In contrast, previous published studies showed that blood collection using EDTA tubes or leukocyte purification was accompanied by profound changes in mRNA expression. 15,16 In this study, the conducted analyses have shown the interest provided by the combined approach (Set-1 þ 2) in comparison with that provided by individual sets (Set- Figure 5 The two gene networks (a, b) derived from the 12 genes, which composed the IFN signature, using the IPA software. Edges (gene relationship) are displayed with labels that describe the nature of the relationship between nodes (genes).…”

Section: Discussionmentioning

confidence: 78%

Specific gene expression signature associated with development of autoimmune type-I diabetes using whole-blood microarray analysis

et al. 2010

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Understanding the pathogenesis of type-I diabetes (T1D) is hindered in humans by the long autoimmune process occurring before clinical onset and by the difficulty to study the pancreas directly. Alternatively, exploring body fluids and particularly peripheral blood can provide some insights. Indeed, circulating cells can function as 'sentinels', with subtle changes in gene expression occurring in association with disease. Therefore, we investigated the gene expression profiles of circulating blood cells using Affymetrix microarrays. Whole-blood samples from 20 first-degree relatives of T1D children with autoimmune diabetes-related antibodies, 19 children immediately after the onset of clinical T1D and 20 age-and sex-matched healthy controls were collected in PAXgene tubes. A global gene expression analysis with MDS approach allowed the discrimination of pre-diabetic subjects, diabetic patients and healthy controls. Univariate statistical analysis highlighted 107 distinct genes differently expressed between these three groups. Two major gene expression profiles were characterized, including type-I IFN-regulated genes and genes associated with biosynthesis and oxidative phosphorylation. Our results showed the presence of early functional modifications associated with T1D, which could help to understand the disease and suggest possible avenues for therapeutic interventions.

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“…Thus, in the current study, no additional ex vivo procedures, such as incubations and density gradient centrifugations, were performed. These procedures have been shown to change gene expression pattern dramatically (Riches et al 1992, Hartel et al 2001. Instead, we stabilized RNA immediately on blood collection, allowing accurate in vivo investigation of gene expression profile in leukocytes, as most of the mRNA in whole blood is considered to originate from leukocytes.…”

Section: Discussionmentioning

confidence: 99%

Studies on estrogen receptor (ER) α and β responses on gene regulation in peripheral blood leukocytes in vivo using selective ER agonists

Stygar¹,

Masironi²,

Eriksson³

et al. 2007

Journal of Endocrinology

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Major reproductive events such as menstruation, ovulation, implantation, and cervical ripening are characterized by an increased number of invading leukocytes in the tissues. Sex steroid hormones, particularly estrogens, play an important role in these dynamic changes in the female reproductive tract. Estrogens have also been implicated in the pathogenesis of many common pathological conditions associated with leukocyte infiltration and immunological dysfunction, such as autoimmune diseases and atherosclerosis. Although the two estrogen receptor (ER) subtypes, ERa and ERb, have been found in different leukocyte populations in tissues and in peripheral blood, there is still very little known about functional activity and importance of ERs in blood cells. To elucidate the different roles for ERa and ERb in peripheral blood leukocytes, we used microarray gene expression profiling of rat peripheral blood leukocytes subjected to in vivo treatment with estradiol (E 2 ), the selective ERa agonist 4,4 0 ,4 00 -(4-propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT), and the selective ERb agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DPN). We report the identification of genes that were commonly regulated by E 2 , PPT, and DPN, and genes that were regulated either by the ERa or ERb agonist. Further confirmatory analyses of the selected regulated genes 12-lipoxygenase, fibulin-1, furin, and calgranulin B are also presented. These results were then compared with those from the uterine tissue of the same animals. Our study demonstrates that peripheral blood leukocytes are responsive to estrogens. E 2 and selective ERa and ERb agonists regulate a number of genes that may contribute to inflammation and remodeling of the extracellular matrix.

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Ex vivo induction of cytokine mRNA expression in human blood samples

Cited by 112 publications

References 24 publications

Evaluation of Interleukin-2 mRNA in Whole Blood as a Parameter for Monitoring Cyclosporine Pharmacodynamics

Evaluation of Interleukin-2 mRNA in Whole Blood as a Parameter for Monitoring Cyclosporine Pharmacodynamics

Specific gene expression signature associated with development of autoimmune type-I diabetes using whole-blood microarray analysis

Studies on estrogen receptor (ER) α and β responses on gene regulation in peripheral blood leukocytes in vivo using selective ER agonists

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