Ex vivo measurement of tissue distribution of a nitroxide radical after intravenous injection and its in vivo imaging using a rapid scan ESR-CT system

Togashi, Hitoshi; Matsuo, Taku; Shinzawa, Haruhide; Takeda, Yoshio; Shao, Li; Oikawa, Kazuo; Kamada, Hitoshi; Takahashi, Tsuneo

doi:10.1016/s0730-725x(99)00122-8

Cited by 21 publications

(19 citation statements)

References 13 publications

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“…ESR-CT imaging using carbamoyl-PROXYL as a spin probe has revealed areas of high intensity in the kidney and liver (Togashi et al, 2000b). In our experiment, the ESR spectrum was measured in the upper abdominal position where the liver is located.…”

Section: Discussionmentioning

confidence: 97%

Measurement of radical-scavenging ability in hepatic metallothionein of rat using in vivo electron spin resonance spectroscopy

et al. 2005

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Section: Discussionmentioning

confidence: 97%

Measurement of radical-scavenging ability in hepatic metallothionein of rat using in vivo electron spin resonance spectroscopy

et al. 2005

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“…Consequently the measurement of the EPR signal decay is equal to the measurement of organ-reducing activity against radicals, which is a major factor of organ antioxidative activity [5]. In addition, this paramagnetic loss of nitroxyl radicals does not occur through reduction with blood components but rather following their transport to various organs [9]. Thus, the altered tissue antioxidant activity in organs results in the prolongation of their EPR signal decay.…”

Section: In Vivo Epr Measurements Of Redox Status Using the Spin-probmentioning

confidence: 99%

Electron Paramagnetic Resonance Imaging of Oxidative Stress in Renal Disease

Hirayama

Nagase²

2006

Nephron Clin Pract

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The importance of analyzing the kinetics of reactive oxygen species or related substances in vivo is increasing. Electron paramagnetic resonance (EPR) is currently a powerful method for in vivo, non-invasive analysis of oxidative stress. We have applied EPR imaging for murine renal ischemia-reperfusion injury, as a model of acute renal damage, and NF-E2-related factor 2 (Nrf2)-deficient mice, a model for chronic progressive renal disease. In the ischemia-reperfusion model, EPR imaging revealed that the renal radical-reducing activity showed only partial recovery when serum creatinine and BUN have recovered. In the Nrf2-deficient mice, we have revealed that the impaired antioxidant activity is brought by both Nrf2 deficiency and the aging process and may play a key role in the onset of autoimmune nephritis in this model. In addition, EPR imaging is recently being applied to the redox analysis of several nephrosis models, hypertensive rats and streptozotocin-induced diabetic rats. This article summarizes the nephrological application of EPR imaging and in vivo EPR.

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“…3-Carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (carbamoyl-PROX-YL) was used to study hepatic oxidative stress [36]. In parallel it is also possible to perform anatomic analysis by following the distribution of the spin traps in different organs [37,38]. Further aspects of in vivo ESR measurements are reviewed by Fuji and Berliner [39].…”

Section: Spin Trappingmentioning

confidence: 99%

Electron spin resonance - A spectroscopic method for determining the antioxidative activity

Rohn¹,

Kroh

2005

Mol. Nutr. Food Res.

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Ex vivo measurement of tissue distribution of a nitroxide radical after intravenous injection and its in vivo imaging using a rapid scan ESR-CT system

Cited by 21 publications

References 13 publications

Measurement of radical-scavenging ability in hepatic metallothionein of rat using in vivo electron spin resonance spectroscopy

Measurement of radical-scavenging ability in hepatic metallothionein of rat using in vivo electron spin resonance spectroscopy

Electron Paramagnetic Resonance Imaging of Oxidative Stress in Renal Disease

Electron spin resonance - A spectroscopic method for determining the antioxidative activity

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