Ex vivo microangioCT: Advances in microvascular imaging

Hlushchuk, Ruslan; Haberthür, David; Djonov, Valentin

doi:10.1016/j.vph.2018.09.003

Cited by 19 publications

(18 citation statements)

References 35 publications

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“…S7 in Supplement 1. It should be highlighted that in our measurements, unlike in other x-ray studies on visualizing the renal vascular network [40][41][42][43], neither contrast agent nor blood was present in the vessels, and the segmentation was solely possible because of the high image quality provided by SBI phase tomography.…”

Section: Three-dimensional Analysis and Segmentation Of Complex Nementioning

confidence: 95%

X-ray phase tomography with near-field speckles for three-dimensional virtual histology

Zdora¹,

Thibault²,

Kuo³

et al. 2020

Optica

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High-contrast, high-resolution imaging of biomedical specimens is indispensable for studying organ function and pathologies. Conventional histology, the gold standard for soft-tissue visualization, is limited by its anisotropic spatial resolution, elaborate sample preparation, and lack of quantitative image information. X-ray absorption or phase tomography have been identified as promising alternatives enabling non-destructive, distortion-free three-dimensional (3D) imaging. However, reaching sufficient contrast and resolution with a simple experimental procedure remains a major challenge. Here, we present a solution based on x-ray phase tomography through speckle-based imaging (SBI). We demonstrate on a mouse kidney that SBI delivers comprehensive 3D maps of hydrated, unstained soft tissue, revealing its microstructure and delivering quantitative tissue-density values at a density resolution of better than 2 mg/cm 3 and spatial resolution of better than 8 µm. We expect that SBI virtual histology will find widespread application in biomedicine and will open up new possibilities for research and histopathology.

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Section: Three-dimensional Analysis and Segmentation Of Complex Nementioning

confidence: 95%

X-ray phase tomography with near-field speckles for three-dimensional virtual histology

Zdora¹,

Thibault²,

Kuo³

et al. 2020

Optica

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“…Among the existing CCAs, Microfil and barium sulphate (BaSO 4 ) are the most common (Table 1). µAngiofil [48][49][50] has also been used as a newly emerging polymerizing CCA. All three of these CCAs allow to create vascular casts for 3D imaging and skeletonization of the vasculature.…”

Section: Spatial Distribution and Morphometrics Of The Vascular Treementioning

confidence: 99%

A Review of Ex Vivo X-ray Microfocus Computed Tomography-Based Characterization of the Cardiovascular System

Leyssens

Pestiaux

Kerckhofs

2021

IJMS

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Cardiovascular malformations and diseases are common but complex and often not yet fully understood. To better understand the effects of structural and microstructural changes of the heart and the vasculature on their proper functioning, a detailed characterization of the microstructure is crucial. In vivo imaging approaches are noninvasive and allow visualizing the heart and the vasculature in 3D. However, their spatial image resolution is often too limited for microstructural analyses, and hence, ex vivo imaging is preferred for this purpose. Ex vivo X-ray microfocus computed tomography (microCT) is a rapidly emerging high-resolution 3D structural imaging technique often used for the assessment of calcified tissues. Contrast-enhanced microCT (CE-CT) or phase-contrast microCT (PC-CT) improve this technique by additionally allowing the distinction of different low X-ray-absorbing soft tissues. In this review, we present the strengths of ex vivo microCT, CE-CT and PC-CT for quantitative 3D imaging of the structure and/or microstructure of the heart, the vasculature and their substructures in healthy and diseased state. We also discuss their current limitations, mainly with regard to the contrasting methods and the tissue preparation.

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“…As such, the smallest branches are not visible, resulting in some arterial and venous trees not to be connected in the macroscopic figures. Furthermore, the use of Microfil® is limited to ex vivo analysis of specimens, hampering longitudinal studies in one animal 39 . Although these limitations exist, visualization of vessels after Microfil® preservation remains a highly advanced yet relatively simple technique with a broad application range to evaluate vessel volume and distributions in any organ or animal model 12 .…”

Section: Discussionmentioning

confidence: 99%

Microcomputed analysis of nerve angioarchitecture after combined stem cell delivery and surgical angiogenesis to nerve allograft

Saffari

Mathot

Thaler

et al. 2021

Journal of Plastic, Reconstructive & Aesthetic Surgery

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Ex vivo microangioCT: Advances in microvascular imaging

Cited by 19 publications

References 35 publications

X-ray phase tomography with near-field speckles for three-dimensional virtual histology

X-ray phase tomography with near-field speckles for three-dimensional virtual histology

A Review of Ex Vivo X-ray Microfocus Computed Tomography-Based Characterization of the Cardiovascular System

Microcomputed analysis of nerve angioarchitecture after combined stem cell delivery and surgical angiogenesis to nerve allograft

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