Ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine

Abstract

Objective:To assess the influence of final irrigation protocols with chlorhexidine in the coronal leakage of Enterococcus faecalis in filled root canals.Material and Methods:Seventy single-root canals from extracted teeth were prepared using ProTaper instruments. The irrigation protocol accomplished an alternating irrigation with 5 mL of 2.5% sodium hypochlorite (NaOCl) and 17% EDTA between each file. The teeth were randomly divided into four experimental groups (n=15) according to the final irrigation regimen: group 1, without final irrigation; group 2, irrigation with 10 mL 2.0% chlorhexidine (CHX); group 3, with a final application of EC40TM; and group 4, irrigation with the combination (1:1) of 0.2% CHX + 0.1% cetrimide (CTR). All the teeth were mounted in a two-chamber apparatus and the coronal access was exposed to E. faecalis. The presence of turbidity in the BHI broth over a period of 180 days was observed. The Friedman test was used for statistical analysis.Results:EC40TM varnish showed the least leakage at 180 days, and was statistically similar to 2% CHX. No significant differences were observed between the group without final irrigation and the 2% CHX group or 0.2% CHX + 0.1% CTR.Conclusions:In this ex vivo study, EC40TM showed the longest delayed coronal leakage of E. faecalis, although without significant differences from 2% CHX.

Solubility and bacterial sealing ability of MTA and root-end filling materials

“…The bacterial leakage used for this study presents some limitations related to difficulty of standardization 10 , 30 . However, it allows the comparison between the sealing ability of different materials 20 , 22 - 24 . Also, this methodology has clinical relevance, simulating clinical use of root-end filling material 19 , 24 , 26 .…”

“…Both the dimensional change and solubility may be directly related to material sealing ability. Several studies evaluate the bacterial leakage in different methodologies 20 , 22 - 24 . These studies suggest that proper dimensional stability and insolubility contribute to a lower degree of bacterial and fluid leakage.…”

Effect of curing conditions on physical and chemical properties of MTA+

“…It has been suggested (Orosco et al 2010, Navarro-Escobar et al 2013, Oliveira et al 2013, Prado et al 2014) that dimensional stability and insolubility prevent microbial and fluid leakage efficiently. According to ANSI/ADA no 57 (ANSI/ADA), ISO 6876 clause 4.3.6, the weight loss of each specimen is expressed as the percentage of the original mass and the ideal is for this to be less than 3%.…”

The Immunomodulatory, Antimicrobial and Bactericidal Efficacy of Commonly Used Commercial Household Disinfectants, Sterilizers and Antiseptics<i> in </i><i>V</i><i>itro</i>: Putative Anti-Inflammatory Infection Control Mechanisms and Comparative Biochemical Analysis of the Microbial Growth of Gram-Positive Bacteria

“…These results bolster the common observations that commercially available household products are effective antimicrobials at various levels, but that professional advertising is less than accurate and consumer's attention should be revisited and redirected. The choice of any of those products as common commodities essentially remains that of the consumer [1][2][3][4][5][25][26][27][28][29][30][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][91][92][93][94][95][96][97][98][99][100][101][102][103][104][105][116][117][118][119]…”