Exacerbation of thrombotic events by diesel exhaust particle in mouse model of hypertension

Nemmar, Abderrahim; Zia, Shaheen; Subramaniyan, Deepa; Fahim, Mohamed A.; Ali, Badreldin H.

doi:10.1016/j.tox.2011.03.018

Cited by 38 publications

(42 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The prothrombin time (PT) was measured [21][22][23] on freshly collected, platelet-poor plasma with human relipidated recombinant thromboplastin (RecombiPlasTin; Instrumentation Laboratory, Orangeburg, NY, USA) in combination with a Merlin coagulometer (MC 1 VET; Merlin Medical, Lemgo, Germany)]. Activated partial thromboplastin time (aPTT) was measured [21][22][23] with automated aPTT reagent (bioMerieux, Inc., Durham, NC, USA) using a Merlin coagulometer (MC 1 VET; Merlin Medical). Normal plasma used as reference for both the PT and aPTT was prepared by pooling equal portions of platelet-poor plasmas from the blood of six untreated mice.…”

Section: In Vitro Measurement Of Prothrombin Time and Activated Partimentioning

confidence: 99%

Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammation

Nemmar¹,

Albarwani²,

Beegam³

et al. 2014

IJN

Self Cite

View full text Add to dashboard Cite

Amorphous silica nanoparticles (SiNPs) are being used in biomedical, pharmaceutical, and many other industrial applications entailing human exposure. However, their potential vascular and systemic pathophysiologic effects are not fully understood. Here, we investigated the acute (24 hours) systemic toxicity of intraperitoneally administered 50 nm and 500 nm SiNPs in mice (0.5 mg/kg). Both sizes of SiNPs induced a platelet proaggregatory effect in pial venules and increased plasma concentration of plasminogen activator inhibitor-1. Elevated plasma levels of von Willebrand factor and fibrinogen and a decrease in the number of circulating platelets were only seen following the administration of 50 nm SiNPs. The direct addition of SiNPs to untreated mouse blood significantly induced in vitro platelet aggregation in a dose-dependent fashion, and these effects were more pronounced with 50 nm SiNPs. Both sizes of SiNPs increased lactate dehydrogenase activity and interleukin 1β concentration. However, tumor necrosis factor α concentration was only increased after the administration of 50 nm SiNPs. Nevertheless, plasma markers of oxidative stress, including 8-isoprostane, thiobarbituric acid reactive substances, catalase, and glutathione S-transferase, were not affected by SiNPs. The in vitro exposure of human umbilical vein endothelial cells to SiNPs showed a reduced cellular viability, and more potency was seen with 50 nm SiNPs. Both sizes of SiNPs caused a decrease in endothelium-dependent relaxation of isolated small mesenteric arteries. We conclude that amorphous SiNPs cause systemic inflammation and coagulation events, and alter vascular reactivity. Overall, the effects observed with 50 nm SiNPs were more pronounced than those with 500 nm SiNPs. These findings provide new insight into the deleterious effect of amorphous SiNPs on vascular homeostasis.

show abstract

Section: In Vitro Measurement Of Prothrombin Time and Activated Partimentioning

confidence: 99%

Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammation

Nemmar¹,

Albarwani²,

Beegam³

et al. 2014

IJN

Self Cite

View full text Add to dashboard Cite

show abstract

“…After that, either CeO 2 NPs (1, 5, or 25 μg/mL) or saline (control) was added for another 3 minutes, and then PT was measured as previously described. 25,30,31 The aPTT was measured with automated aPTT reagent (BioMerieux, Durham, NC, USA) by using an MC 1 VET (Merlin). Briefly, the plasma (n=4-6 per group) was incubated for 3 minutes at 37°C.…”

Section: Experimental Pial Cerebral Arteriole Thrombosis Modelmentioning

confidence: 99%

“…After that, either CeO 2 NPs (1, 5, or 25 μg/mL) or saline (control) was added for another 3 minutes, and then aPTT was measured as previously described. 25,30,31 Normal plasma was used as reference for both the PT and aPTT.…”

Section: Experimental Pial Cerebral Arteriole Thrombosis Modelmentioning

confidence: 99%

The acute pulmonary and thrombotic effects of cerium oxide nanoparticles after intratracheal instillation in mice

Al-Salam¹,

Beegam²,

Yuvaraju³

et al. 2017

IJN

Self Cite

View full text Add to dashboard Cite

Cerium oxide nanoparticles (CeO 2 NPs), used as a diesel fuel catalyst, can be emitted into the ambient air, resulting in exposure to humans by inhalation. Recent studies have reported the development of lung toxicity after pulmonary exposure to CeO 2 NPs. However, little is known about the possible thrombotic effects of these NPs. The present study investigated the acute (24 hours) effect of intratracheal (IT) instillation of either CeO 2 NPs (0.1 or 0.5 mg/kg) or saline (control) on pulmonary and systemic inflammation and oxidative stress and thrombosis in mice. CeO 2 NPs induced a significant increase of neutrophils into the bronchoalveolar lavage (BAL) fluid with an elevation of tumor necrosis factor α (TNFα) and a decrease in the activity of the antioxidant catalase. Lung sections of mice exposed to CeO 2 NPs showed a dose-dependent infiltration of inflammatory cells consisting of macrophages and neutrophils. Similarly, the plasma levels of C-reactive protein and TNFα were significantly increased, whereas the activities of catalase and total antioxidant were significantly decreased. Interestingly, CeO 2 NPs significantly and dose dependently induced a shortening of the thrombotic occlusion time in pial arterioles and venules. Moreover, the plasma concentrations of fibrinogen and plasminogen activator inhibitor-1 were significantly elevated by CeO 2 NPs. The direct addition of CeO 2 NPs (1, 5, or 25 μg/mL) to mouse whole blood, collected from the inferior vena cava, in vitro neither caused significant platelet aggregation nor affected prothrombin time or partial thromboplastin time, suggesting that the thrombotic events observed in vivo may have resulted from systemic inflammation and/or oxidative stress induced by CeO 2 NPs. This study concludes that acute pulmonary exposure to CeO 2 NPs induces pulmonary and systemic inflammation and oxidative stress and promotes thrombosis in vivo.

show abstract

“…The PT was measured [13][14][15] on freshly collected, plateletpoor plasma with human relipidated recombinant thromboplastin (Recombiplastin; Instrumentation Laboratory, Orangeburg, NY, USA) in combination with a Merlin coagulometer [MC 1 VET (Merlin, Lemgo, Germany)]. Briefly, the plasma was incubated for 3 min at 37 C, and then various concentrations of betaine [0.01 (n ¼ 5), 0.1 (n ¼ 5) and 1 (n ¼ 5) mg/mL)] in saline (0.9%) or saline alone (control, n ¼ 5) were added for another 3 min and then PT was measured as previously described.…”

Section: In Vitro Measurement Of Pt and Aptt In Plasma In Vitromentioning

confidence: 99%

“…Briefly, the plasma was incubated for 3 min at 37 C, and then various concentrations of betaine [0.01 (n ¼ 5), 0.1 (n ¼ 5) and 1 (n ¼ 5) mg/mL)] in saline (0.9%) or saline alone (control, n ¼ 5) were added for another 3 min and then PT was measured as previously described. [13][14][15] aPTT was measured [13][14][15] with automated aPTT reagent [bioMerieux (Durham, NC, USA) using a Merlin coagulometer the MC 1 VET (Merlin, Lemgo, Germany)]. Briefly, the plasma was incubated for 3 min at 37 C, and then various concentrations of betaine [0.01 (n ¼ 4), 0.1 (n ¼ 4) and 1 (n ¼ 4) mg/mL)] in saline (0.9%) or saline alone (control, n ¼ 6) were added for another 3 min and then aPTT was assessed as previously described.…”

Section: In Vitro Measurement Of Pt and Aptt In Plasma In Vitromentioning

confidence: 99%

Betaine (N,N,N-trimethylglycine) averts photochemically-induced thrombosis in pial microvesselsin vivoand platelet aggregationin vitro

Yuvaraju

Beegam

Ali

2015

Exp Biol Med (Maywood)

Self Cite

View full text Add to dashboard Cite

Betaine (N,N,N-trimethylglycine) is an important food component with established health benefits through its homocysteinelowering effects, and is used to lower total homocysteine concentration in plasma of patients with homocystinuria. It is well established that hyperhomocysteinemia is an established risk factor for cardiovascular disease and stroke. However, the possible protective effect of betaine on coagulation events in vivo and in vitro has thus far not been studied. Betaine was given to mice at oral doses of either 10 mg/kg (n ¼ 6) or 40 mg/kg (n ¼ 6) for seven consecutive days, and control mice (n ¼ 6) received water only. The thrombotic occlusion time in photochemically induced thrombosis in pial arterioles was significantly delayed in mice pretreated with betaine at doses of 10 mg/kg (P < 0.001) and 40 mg/kg (P < 0.01). Similar effects were observed in pial venules with 10 mg/kg (P < 0.05) and 40 mg/kg (P < 0.05) betaine. In vitro, in whole blood samples collected from untreated mice (n ¼ 3-5), betaine (0.01-1 mg/mL) significantly reversed platelet aggregation induced by adenosine diphosphate (5 mM). The number of circulating platelets and plasma concentration of fibrinogen in vivo were not significantly affected by betaine pretreament compared with the control group. Lipid peroxidation (LPO) in mice pretreated with betaine was significantly reduced compared with the control group. Moreover, betaine (0.01-1 mg/mL) caused a dose-dependent and significant prolongation of PT (n ¼ 5) and aPTT (n ¼ 4-6). In conclusion, our data show that betaine protected against coagulation events in vivo and in vitro and decreased LPO in plasma.Keywords: Betaine, platelets, thrombosis, mice, lipid peroxidation Experimental Biology and Medicine 2015; 240: 955-960.

show abstract

Exacerbation of thrombotic events by diesel exhaust particle in mouse model of hypertension

Cited by 38 publications

References 53 publications

Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammation

Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammation

The acute pulmonary and thrombotic effects of cerium oxide nanoparticles after intratracheal instillation in mice

Betaine (N,N,N-trimethylglycine) averts photochemically-induced thrombosis in pial microvesselsin vivoand platelet aggregationin vitro

Contact Info

Product

Resources

About