Exact anhydride epoxy percentages for electron microscopy embedding (Epon)

Burke, C. N.; Geiselman, C. Wayne

doi:10.1016/s0022-5320(71)80092-8

Cited by 53 publications

(13 citation statements)

References 2 publications

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“…Numerous reports outline the procedures for making these calculations (e.g., Burk and Geiselman, 1971; see also Glauert, 1991).…”

Section: Anhydride/epoxy (Am) Ratiosmentioning

confidence: 99%

Artifacts caused by dehydration and epoxy embedding in transmission electron microscopy

Mollenhauer

1993

Microscopy Res & Technique

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Epoxy resins are the principal embedding media for the preservation of tissues to be sectioned and examined by transmission electron microscopy. Their primary advantages are good ultrastructural preservation, little or no shrinkage, ease of sectioning, and reasonable stability in the electron beam. However, epoxy resins also have disadvantages; namely, some are toxic, they may mask antigenic sites to a greater extent than do some other embedding resins, and they do not penetrate tissues as well as less viscous embedding formulations. Some unusual characteristics may also be revealed, for example, as shrinkage of organelles, as problems in poststaining sections, and as movement of tissue elements within the block and section. Some of the properties of epoxy resins are discussed in this report.

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“…Numerous reports outline the procedures for making these calculations (e.g., Burk and Geiselman, 1971; see also Glauert, 1991).…”

Section: Anhydride/epoxy (Am) Ratiosmentioning

confidence: 99%

Artifacts caused by dehydration and epoxy embedding in transmission electron microscopy

Mollenhauer

1993

Microscopy Res & Technique

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“…Ind., Inc.), and ERL-4206 (Polysciences, Inc.). With the first two media, the following ratios of mixtures A and B (Burke and Geiselman, 1971) were used: 5.5:6.2, 654.9, and 4.4r7.4. LX-112 was used only in the ratio of 6:4.…”

Section: Materials a N D Methodsmentioning

confidence: 99%

Effects of different epoxies on avoiding wrinkles in thin sections of botanical specimens

Pihakaski

Suoranta

1985

J. Elec. Microsc. Tech.

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Folds or wrinkles appear frequently in thin sections cut from botanical material embedded in epoxy resin such as Epon 812 (Polysciences) or EM-bed-812 (Electron Microscopy Sciences). Small wrinkles may occur more or less perpendicular to the cutting direction and mostly across the cell wall, causing problems in the examination of the ultrastructure. We studied the occurrence of wrinkles and folds by methodological sectioning, with regard to block hardness, use of Formvar-support film, and chloroform vapor. Methodological sectioning or changing the block hardness did not reduce the occurrence of wrinkles in sections. Chloroform vapor improved the result as did also the use of uncoated grids. Wrinkles and folds could be avoided completely by embedding the specimens in LX-112 (Ladd) or ERL-4206 (Polysciences) lowviscosity epoxy resins, in which case Formvar-coated grids could be used, and chloroform vapor was not necessary.

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“…However, a higher ratio is necessary when curing occurs at temperatures in excess of 95°C (Coulter, 1967). The epoxide equivalent (WPE) of the Epon 812 that we have used varied from 150 to 160 and the proportions of the various components in the embedding mixture were adjusted to retain the 0-76 A:E ratio by application of the formulae and tables previously published (Coulter, 1967;Burke and Geiselman, 1971). The WPE of the Epon 812 in the formula stated here was 156.…”

Section: Methodsmentioning

confidence: 99%