We obtain the mean spin polarization vector of spin 1/2 particles in a relativistic fluid at Local Thermal Equilibrium (LTE) in different Pseudo-Gauges (PGs), i.e. with different choices for the decomposition of orbital and spin angular momentum. The spin polarization obtained in the canonical PG differs from the one obtained in the Belinfante PG. We found that this difference can not be written by replacing the thermal vorticity with the spin potential in the usual polarization formula. Other PG choices affect the contribution of thermal shear to the spin polarization. Therefore, the choice of a PG affects the predictions for the polarization measured in heavy-ion collisions. In general, we show that the Wigner function of a non-interacting Dirac field at LTE is affected by the PG transformations. We also calculate the explicit expressions of the mean axial current and we obtain that even the axial vortical effect conductivity depend on the PG.