Examination of the bactericidal and opsonic activity of normal human serum for a mucoid and nonmucoid strain ofPseudomonas aeruginosa

Borowski, Robert S.; Schiller, Neal L.

doi:10.1007/bf01567129

Cited by 13 publications

(8 citation statements)

References 15 publications

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“…Strains of E. coli isolated from cases of bacteraemia are more often serum resistant than are gut strains (Vosti and Randall, 1970;McCabe et al, 1978). Serum resistance has also been associated with virulence in several other organisms, including Serratia marcescens (Simberkoff et al, 1976), Pseudomonas aeruginosa (Borowski and Schiller, 1983), Neisseria gonorrhoeae (Schoolnik et al, 1976;Eisenstein et al, 1977), and Vibrio vulnijicus (Johnson et al, 1984). In this study, strains of K .…”

Section: Discussionmentioning

confidence: 67%

Bactericidal activity of human serum against strains of Klebsiella from different sources

Benge

1988

Journal of Medical Microbiology

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Summary.One hundred and eighty strains of Klebsiella were tested for sensitivity to pooled normal human serum, capsular type and faecal coliform reaction. Strains of K . pneumoniae isolated from clinical infections were more likely to be serum resistant than those from the gut flora or environmental sources. For K . oxytoca strains, there was no significant correlation between serum sensitivity and source of isolation. Of 60 strains of K . oxytoca tested, 19 (32%) were serum resistant, as were 27 (23%) of 120 strains of K . pneumoniae. Strains of K . pneumoniae from human sources gave positive reactions in the faecal coliform test more frequently than strains from environmental sources. There was no correlation between a positive faecal coliform reaction and resistance to the bactericidal effect of human serum. Strains of capsular type K21 were isolated more frequently from clinical infections and were more often serum resistant than other strains. Serum resistance appears to be a virulence factor in strains of K . pneumoniae but does not account for the difference in pathogenicity between K . pneumoniae and K . oxytoca.

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Section: Discussionmentioning

confidence: 67%

Bactericidal activity of human serum against strains of Klebsiella from different sources

Benge

1988

Journal of Medical Microbiology

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“…FRD1 and 144M are mucoid strains of P. aeruginosa isolated from the lungs of patients with CF, and FRD2 and 144NM represent spontaneously derived non‐mucoid derivatives of these strains, respectively (10, 11).…”

Section: Methodsmentioning

confidence: 99%

Alginate lyase enhances antibiotic killing of mucoid Pseudomonas aeruginosa in biofilms

Alkawash

Soothill

Schiller

2006

APMIS

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249

148

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Once mucoid (alginate-producing) strains of Pseudomonas aeruginosa have become established in the respiratory tracts of cystic fibrosis patients they can rarely be eliminated by antibiotic treatment alone; we have investigated, in an in vitro biofilm system, the putative role of co-administration of alginate lyase with antibiotic. Biofilms were maintained in continuous flow culture in a medium resembling sputum from CF patients. Antibiotics and/or alginate lyase were added to some of the cultures. Biofilms of two mucoid CF strains of P. aeruginosa were, in most cases, not eradicated by a one-week course of treatment with 64 microg/ml of gentamicin; the same concentration of gentamicin, under the same conditions, led to the apparent elimination of all biofilms of non-mucoid derivatives of these strains. When alginate lyase and gentamicin were administered together the apparent elimination of mucoid bacteria from biofilms was achieved, whereas the mucoid bacteria in most control biofilms treated only with gentamicin persisted. Ceftazidime treatment of biofilms was more effective against those containing the non-mucoid strains than those with mucoid strains. These studies support the view that co-administration of antibiotics with alginate lyase, which degrades the exopolysaccharide produced by mucoid strains of P aeruginosa, might benefit CF patients by increasing the efficacy of antibiotic in the respiratory tract.

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“…IgM-and IgG-rich pools (pools A and B, respectively) were obtained by fractionation of FHS on a Bio-Gel A column (1.5 m, 100 to 200 mesh; Bio-Rad Laboratories, Richmond, Calif.) and characterized as to immunoglobulin content as described previously (4,32). Pool A contained 39 mg of IgM per dl and no detectable IgG or IgA, whereas pool B contained 25 mg of IgM, 720 mg of IgG, and 105 mg of IgA per dl.…”

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confidence: 99%

Serum sensitivity of a Pseudomonas aeruginosa mucoid strain

Schiller¹,

Alazard²,

Borowski³

1984

Infect Immun

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The susceptibility of Pseudomonas aeruginosa 144M (a mucoid strain isolated from the sputum of a cystic fibrosis patient) to the bactericidal activity of pooled fresh normal human serum (FHS) was examined. FHS at concentrations of greater than or equal to 2.5% was capable of killing greater than 95% of strain 144M. Strain 144M was killed by FHS in a dose-dependent manner. Although either immunoglobulin M (IgM) or IgG was bactericidal in the presence of complement, IgM was about 10 times as effective as IgG. However, optimal killing activity required both IgM and IgG and complement, activated by the classical pathway. A role for lysozyme in the killing of 144M was demonstrated only when low concentrations of FHS were used. In contrast to 144M, P. aeruginosa strains 144NM and 144M(SR) were totally resistant to FHS at all of the concentrations tested (up to 50%). Neither the FHS susceptibility of 144M nor the FHS resistance of 144NM or 144M(SR) was altered by choice of growth medium, growth phase, or temperature of growth. Results of absorption studies with whole organisms, isolated outer membrane preparations, or lipopolysaccharide (LPS) from each strain suggest that the antigen(s) which binds the bactericidal immunoglobulins is accessible on the surface of 144M but not on the surface of 144NM or 144M(SR), is insensitive to trypsin treatment, and is believed to be LPS. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the three LPS preparations demonstrated that 144M LPS contained primarily lipid-A-core polysaccharide components, whereas the LPS from 144NM and 144M(SR) were heterogeneous, with various degrees of O-side-chain substitution. These results suggest that at least one target for bactericidal antibody on the surface of 144M is contained in the rough LPS of this strain.

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Examination of the bactericidal and opsonic activity of normal human serum for a mucoid and nonmucoid strain ofPseudomonas aeruginosa

Cited by 13 publications

References 15 publications

Bactericidal activity of human serum against strains of Klebsiella from different sources

Bactericidal activity of human serum against strains of Klebsiella from different sources

Alginate lyase enhances antibiotic killing of mucoid Pseudomonas aeruginosa in biofilms

Serum sensitivity of a Pseudomonas aeruginosa mucoid strain

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