Examination of the effects of dry storage stress on mitochondrial energy synthesis in the scallop Mizuhopecten yessoensis

Yin, Zhongzhuan; Li, Man; Cai, Yan; Lin, Qin; Yuan, Chunhong; Tian, Yuanyong

doi:10.1016/j.aquaculture.2023.739770

Cited by 3 publications

(7 citation statements)

References 30 publications

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“…NADH dehydrogenase is one of the key enzymes in the adenosine triphosphate (ATP) transport system, while phytanoyl-CoA dioxygenase is assumed to be involved in the cholesterol synthesis pathway in invertebrates . Yin et al reported that Mizuhopecten yessoensis adjusted its consumption of energy materials from metabolism during dry storage . These findings revealed that lipid metabolism and energy compensation might occur during transportation due to starvation and anoxic stress in crabs .…”

Section: Resultsmentioning

confidence: 99%

“…The respiratory chain, indicative of mitochondrial function in crustaceans, might disrupt energy release within the cell under stress conditions . Previous research has shown that mitochondria function, particularly in terms of electron transport chain, activity can be inhibited by hypoxia stress during dry storage in Mizuhopecten yessoensis, resulting in mitochondrial depolarization …”

Section: Resultsmentioning

confidence: 99%

“…41 Yin et al reported that Mizuhopecten yessoensis adjusted its consumption of energy materials from metabolism during dry storage. 42 These findings revealed that lipid metabolism and energy compensation might occur during transportation due to starvation and anoxic stress in crabs. 43 In addition, early research on P. trituberculatus has shown that crabs convert ammonia to less toxic Gln to withstand ammonia exposure under the catalysis of GS.…”

Section: Metabolic Process-related Depsmentioning

confidence: 88%

“…54 Previous research has shown that mitochondria function, particularly in terms of electron transport chain, activity can be inhibited by hypoxia stress during dry storage in Mizuhopecten yessoensis, resulting in mitochondrial depolarization. 42 Purine nucleoside metabolic, ribonucleoside metabolic, nucleotide phosphorylation, and nucleoside diphosphaterelated processes exhibited upregulation in the early stage and subsequent downregulation in the late stage of transportation. Previous related studies have shown that when aquatic animals are subjected to environmental stress, they use a purine nucleotide catabolism strategy for ammonia detoxification, 56 which leads to the formation of urea (UA).…”

Section: Functional Domain and Subcellular Localizationmentioning

confidence: 99%

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Investigation of the Alternations in the Muscle Quality of Swimming Crab (Ovalipes punctatus) during Cold-Chain Transportation Using Physicochemical and TMT-Based Quantitative Proteomic Analysis

Du,

Wu,

Liao

et al. 2024

J. Agric. Food Chem.

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Physicochemical properties and protein alterations in Ovalipes punctatus during cold-chain transportation were examined via sensory scores, water-holding capacity (WHC), glucose (GLU) content, catalase (CAT) activity, urea nitrogen (UN) content, and tandem mass tag (TMT)-based proteomic analysis. The results revealed that sensory characteristics and texture of crab muscle deteriorated during transportation. Proteomic analysis revealed 442 and 470 different expressed proteins (DEPs) in crabs after 18 h (FC) and 36 h (DC) of transportation compared with live crabs (LC). Proteins related to muscle structure and amino acid metabolism significantly changed, as evidenced by the decreased WHC and sensory scores of crab muscle. Glycolysis, calcium signaling, and peroxisome pathways were upregulated in the FC/LC comparison, aligning with the changes in GLU content and CAT activity, revealing the stress response of energy metabolism and immune response in crabs during 0−18 h of transportation. The downregulated tricarboxylic acid (TCA) cycle and carcinogenesis-reactive oxygen species pathways were correlated with the decreasing trend in CAT activity, suggesting a gradual retardation in both energy and antioxidant metabolism in crabs during 18−36 h of transportation. Furthermore, the regulated purine nucleoside metabolic and nucleoside diphosphate-related processes, with the increasing changes in UN content, revealed the accumulation of metabolites in crabs.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Metabolic Process-related Depsmentioning

confidence: 88%

Section: Functional Domain and Subcellular Localizationmentioning

confidence: 99%

See 2 more Smart Citations

Investigation of the Alternations in the Muscle Quality of Swimming Crab (Ovalipes punctatus) during Cold-Chain Transportation Using Physicochemical and TMT-Based Quantitative Proteomic Analysis

Du,

Wu,

Liao

et al. 2024

J. Agric. Food Chem.

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“…Within the striated muscle of Patinopecten yessoensis, the glucose-succinate pathway serves a crucial function during the middle and late stages (6-12 h) of sustained hypoxia stress that arises from dry storage. Upon careful examination, this pathway releases propionate and acetate through volatilization to prevent material buildup and preserve muscle pH stability, while simultaneously generating ATP and NADH to facilitate glycolysis [30]. Depending on the energy source utilized, PEPCK can be categorized into two classes: ATP-dependent and GTP-dependent.…”

Section: Introductionmentioning

confidence: 99%

The Glucose–Succinate Pathway: A Crucial Anaerobic Metabolic Pathway in the Scallop Chlamys farreri Experiencing Heat Stress

Bao,

Liu,

Sui

et al. 2024

IJMS

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Recently, the increase in marine temperatures has become an important global marine environmental issue. The ability of energy supply in marine animals plays a crucial role in avoiding the stress of elevated temperatures. The investigation into anaerobic metabolism, an essential mechanism for regulating energy provision under heat stress, is limited in mollusks. In this study, key enzymes of four anaerobic metabolic pathways were identified in the genome of scallop Chlamys farreri, respectively including five opine dehydrogenases (CfOpDHs), two aspartate aminotransferases (CfASTs) divided into cytoplasmic (CfAST1) and mitochondrial subtype (CfAST2), and two phosphoenolpyruvate carboxykinases (CfPEPCKs) divided into a primitive type (CfPEPCK2) and a cytoplasmic subtype (CfPEPCK1). It was surprising that lactate dehydrogenase (LDH), a key enzyme in the anaerobic metabolism of the glucose–lactate pathway in vertebrates, was absent in the genome of scallops. Phylogenetic analysis verified that CfOpDHs clustered according to the phylogenetic relationships of the organisms rather than substrate specificity. Furthermore, CfOpDHs, CfASTs, and CfPEPCKs displayed distinct expression patterns throughout the developmental process and showed a prominent expression in muscle, foot, kidney, male gonad, and ganglia tissues. Notably, CfASTs displayed the highest level of expression among these genes during the developmental process and in adult tissues. Under heat stress, the expression of CfASTs exhibited a general downregulation trend in the six tissues examined. The expression of CfOpDHs also displayed a downregulation trend in most tissues, except CfOpDH1/3 in striated muscle showing significant up-regulation at some time points. Remarkably, CfPEPCK1 was significantly upregulated in all six tested tissues at almost all time points. Therefore, we speculated that the glucose–succinate pathway, catalyzed by CfPEPCK1, serves as the primary anaerobic metabolic pathway in mollusks experiencing heat stress, with CfOpDH3 catalyzing the glucose–opine pathway in striated muscle as supplementary. Additionally, the high and stable expression level of CfASTs is crucial for the maintenance of the essential functions of aspartate aminotransferase (AST). This study provides a comprehensive and systematic analysis of the key enzymes involved in anaerobic metabolism pathways, which holds significant importance in understanding the mechanism of energy supply in mollusks.

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Effects of Seasonal Variation in Plumpness, Biochemical Composition from Scallop ( Mizuhopecten yessoensis ) on Vitality Changes During Dry Storage

Cai,

Jiang,

Liu

et al. 2024

Journal of Aquatic Food Product Technology

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Examination of the effects of dry storage stress on mitochondrial energy synthesis in the scallop Mizuhopecten yessoensis

Cited by 3 publications

References 30 publications

Investigation of the Alternations in the Muscle Quality of Swimming Crab (Ovalipes punctatus) during Cold-Chain Transportation Using Physicochemical and TMT-Based Quantitative Proteomic Analysis

Investigation of the Alternations in the Muscle Quality of Swimming Crab (Ovalipes punctatus) during Cold-Chain Transportation Using Physicochemical and TMT-Based Quantitative Proteomic Analysis

The Glucose–Succinate Pathway: A Crucial Anaerobic Metabolic Pathway in the Scallop Chlamys farreri Experiencing Heat Stress

Effects of Seasonal Variation in Plumpness, Biochemical Composition from Scallop ( Mizuhopecten yessoensis ) on Vitality Changes During Dry Storage

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