Examination of the Glycine Betaine-Dependent Methylotrophic Methanogenesis Pathway: Insights Into Anaerobic Quaternary Amine Methylotrophy

Creighbaum, Adam J.; Ticak, Tomislav; Shinde, Shrameeta; Wang, Xin; Ferguson, Donald J.

doi:10.3389/fmicb.2019.02572

Cited by 15 publications

(33 citation statements)

References 76 publications

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“…Thus far, characterized members of the MttB superfamily have been shown to methylate either a supplied free corrinoid cofactor or a corrinoid protein ( 1 , 5 , 6 , 7 , 8 , 9 ). Therefore, we hypothesized that MtyB might also be a corrinoid-dependent methyltransferase but with specificity for γ-butyrobetaine.…”

Section: Resultsmentioning

confidence: 99%

“…Phylogenetic analysis indicates substantial diversity among the many MttB superfamily members encoded in the genome of E. limosum ( Fig. S7 ), which led us to investigate their functional diversity ( 5 , 6 , 7 ). Here, we have shown that even two closely related nonpyrrolysine MttB homologs in the E. limosum genome have modified functionality in such a way as to allow their specialization for use with two distinct but related substrates.…”

Section: Discussionmentioning

confidence: 99%

“…Recent work has shown some of these proteins are quaternary amine (QA)-dependent methyltransferases. Each demethylates a specific QA such as glycine betaine ( 5 , 6 , 7 ), proline betaine ( 8 ), or carnitine ( 9 ) in order to methylate a cognate corrinoid protein. The methylated corrinoid protein is then used to methylate catabolic cofactors such as tetrahydrofolate (THF) in bacteria or coenzyme M in methanogenic archaea.…”

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confidence: 99%

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The MttB superfamily member MtyB from the human gut symbiont Eubacterium limosum is a cobalamin-dependent γ-butyrobetaine methyltransferase

Ellenbogen

Jiang

Kountz

et al. 2021

Journal of Biological Chemistry

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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The MttB superfamily member MtyB from the human gut symbiont Eubacterium limosum is a cobalamin-dependent γ-butyrobetaine methyltransferase

Ellenbogen

Jiang

Kountz

et al. 2021

Journal of Biological Chemistry

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“…Next to the three-component methyltransferase systems for monomethylamine (MtmBCA), dimethylamine (MtbBCA) and trimethylamine (MttBCA) it has been described for M. barkeri that RamA, a 60-kDa monomeric ironsulfur protein, is required for ATP-dependent reductive activation of methylamine:CoM methyl transfer from all three methylamines (Ferguson et al 2009 ). For the betaine consuming methanogen Methanolobus vulcani B1d it has been shown that the organism possesses the methyltransferase MtgB (FKV42_RS08545) that catalyzes betaine-dependent methylation of free cob(I)alamin (Creighbaum et al 2019 ). Further, proteomic analysis revealed that MtgB, a corrinoid binding protein (FKV42_RS08550), a corrinoid reductive activation enzyme (FKV42_RS10455) and a methylcorrinoid:CoM methyltransferase (FKV42_RS10480) were highly abundant when M. vulcani B1d was grown on betaine relative to growth on trimethylamine.…”

Section: Hydrogenotrophic Methanogenesis With Additional Electron Donmentioning

confidence: 99%

Several ways one goal—methanogenesis from unconventional substrates

Kurth

Camp

Welte

2020

Appl Microbiol Biotechnol

128

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Methane is the second most important greenhouse gas on earth. It is produced by methanogenic archaea, which play an important role in the global carbon cycle. Three main methanogenesis pathways are known: in the hydrogenotrophic pathway H 2 and carbon dioxide are used for methane production, whereas in the methylotrophic pathway small methylated carbon compounds like methanol and methylated amines are used. In the aceticlastic pathway, acetate is disproportionated to methane and carbon dioxide. However, next to these conventional substrates, further methanogenic substrates and pathways have been discovered. Several phylogenetically distinct methanogenic lineages ( Methanosphaera , Methanimicrococcus , Methanomassiliicoccus , Methanonatronarchaeum ) have evolved hydrogen-dependent methylotrophic methanogenesis without the ability to perform either hydrogenotrophic or methylotrophic methanogenesis. Genome analysis of the deep branching Methanonatronarchaeum revealed an interesting membrane-bound hydrogenase complex affiliated with the hardly described class 4 g of multisubunit hydrogenases possibly providing reducing equivalents for anabolism. Furthermore, methylated sulfur compounds such as methanethiol, dimethyl sulfide, and methylmercaptopropionate were described to be converted into adapted methylotrophic methanogenesis pathways of Methanosarcinales strains. Moreover, recently it has been shown that the methanogen Methermicoccus shengliensis can use methoxylated aromatic compounds in methanogenesis. Also, tertiary amines like choline ( N , N , N -trimethylethanolamine) or betaine ( N , N , N -trimethylglycine) have been described as substrates for methane production in Methanococcoides and Methanolobus strains. This review article will provide in-depth information on genome-guided metabolic reconstructions, physiology, and biochemistry of these unusual methanogenesis pathways. Key points • Newly discovered methanogenic substrates and pathways are reviewed for the first time. • The review provides an in-depth analysis of unusual methanogenesis pathways. • The hydrogenase complex of the deep branching Methanonatronarchaeum is analyzed.

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“…Similarly, an MtgB ortholog from Methanolobus sp. B1d was determined to transfer the GBT methyl group to coenzyme M, thereby playing a key role in GBT-dependent methylotrophic methanogenesis ( 25 ).…”

Section: Introductionmentioning

confidence: 99%

Bacteria and Archaea Synergistically Convert Glycine Betaine to Biogenic Methane in the Formosa Cold Seep of the South China Sea

Zhang

et al. 2021

mSystems

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Examination of the Glycine Betaine-Dependent Methylotrophic Methanogenesis Pathway: Insights Into Anaerobic Quaternary Amine Methylotrophy

Cited by 15 publications

References 76 publications

The MttB superfamily member MtyB from the human gut symbiont Eubacterium limosum is a cobalamin-dependent γ-butyrobetaine methyltransferase

The MttB superfamily member MtyB from the human gut symbiont Eubacterium limosum is a cobalamin-dependent γ-butyrobetaine methyltransferase

Several ways one goal—methanogenesis from unconventional substrates

Bacteria and Archaea Synergistically Convert Glycine Betaine to Biogenic Methane in the Formosa Cold Seep of the South China Sea

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