Examination of the Mode of Action of Ribavirin against Tobacco Mosaic Virus

Dawson, William O.; Lozoya-Saldaña, Héctor

doi:10.1159/000149537

Cited by 32 publications

(14 citation statements)

References 9 publications

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“…Precursor incorpora tion experiments confirmed that viral RNA and protein syntheses were inhibited. This is in contrast to other inhibitors examined, 2-thiouracil [17] and ribavirin [18], which pre vent early steps of TMV multiplication.…”

Section: Discussioncontrasting

confidence: 82%

Specific Inhibition of Tobacco Mosaic Virus Protein and Single-Stranded RNA Synthesis by Arabinofuranosyladenine

Dawson¹,

Lozoya-Saldaña²

1986

Intervirology

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9-β-D-Arabinofuranosyladenine (ara A) was shown to inhibit a specific step of tobacco mosaic virus (TMV) multiplication. The time course of the ara A-sensitive function occurred ‘late’ coinciding with the period of viral RNA and protein synthesis. When treatment began after viral RNA synthesis was established, 1.0 mMara A inhibited viral single-stranded RNA and viral protein synthesis, but not viral double-stranded RNA synthesis. Viral double-stranded RNA synthesis was inhibited totally only when treatment with ara A began within the first 4 h (before any RNA synthesis was detected), and the degree of inhibition decreased rapidly when treatments began at later times. In contrast, single-stranded TMV RNA synthesis and viral protein synthesis were inhibited throughout the infection. The mode of action of ara A against TMV involves selective inhibition of a function required for single-stranded RNA synthesis and viral protein synthesis without detectable inhibition of RNA or protein synthesis of the host or ongoing viral double-stranded RNA synthesis.

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Section: Discussioncontrasting

confidence: 82%

Specific Inhibition of Tobacco Mosaic Virus Protein and Single-Stranded RNA Synthesis by Arabinofuranosyladenine

Dawson¹,

Lozoya-Saldaña²

1986

Intervirology

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“…Taiwan and chileno by employing sequential thermotherapy at 32°C for a week, 36°C for Direct and immediate suppressive effects of exposure to 40°C on the synthesis of host and viral RNAs and on the activity of viral replicase were demonstrated in cowpea and tobacco plants infected by various viruses. However, when the plants were transferred after a few hours at 40°C to an optimal temperature, host RNA synthesis was resumed immediately, while virus synthesis was delayed (Dawson and Lozoya Saldana 1984). The effect on viral RNA synthesis could probably be magnified if cycles of high and low temperature are repeated, thereby allowing elimination of the virus from growing parts of the plants in relative short periods of time (Stein et al 1991).…”

Section: Thermotherapy Applied To Intact Plantmentioning

confidence: 99%

In vitro production of Indian citrus ringspot virus (ICRSV) free Kinnow plants employing thermotherapy coupled with shoot tip grafting

Sharma

Singh

Rani

et al. 2007

Plant Cell Tiss Organ Cult

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Indian citrus ringspot virus (ICRSV) is known to cause serious disease problem in Kinnow (Citrus nobilis Lour · C. deliciosa Tenora) plants. This work reports the elimination of ICRSV by using thermotherapy coupled with shoot tip grafting in vitro. Nodal segments from infected mother plants (indexed by indirect ELISA and RT-PCR) were treated both in water bath and moist hot air at different temperatures viz. 40, 45 and 50°C for 30, 60 and 120 min and cultured on MS medium containing 2-iP (1 mg/l) and malt extract (800 mg/l). Shoot tips were excised from the nodal sprouts and grafted on to rough lemon (C. jambhiri) rootstock under aseptic conditions. Water bath treatment was found to be more effective as compared to moist hot air treatment as maximum number of ICRSV free plants (36.84%) were obtained by grafting the tips (0.7 mm) taken from the nodal segments treated at 50°C in water bath for 2 h. In an alternate treatment regime, 1-year-old infected plants were kept at various temperatures viz.36, 38 and 40°C in a thermotherapy chamber. Maximum of 60% ICRSV free plants were obtained by grafting the tips (0.7 mm) from the plants placed at 40°C followed by the plants placed at 38°C (59.09%) and the least was observed in case of the plants placed at 36°C (40.74%). Only those plants/plantlets were considered virus free, which showed negative reaction both in Indirect ELISA and RT-PCR.

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“…2-Thiouracil inhibits a step that occurs later than the actinomycin D-sensitive step, but earlier than viral protein or RNA synthesis (Dawson and Schlegel, 1976a;Dawson and Grantham, 1983). Ribovirin and low concentrations of guanidine inhibit with the same kinetics and might inhibit the same process (Dawson, 1975;Dawson and Lozoya-Saldana, 1984). Cycloheximide and arabinofuranosyladenine inhibit a later step that coincides with protein and RNA syntheses (Dawson and Schiegel, 1976a;Dawson and Lozoya-Saldana, 1986).…”

Section: Replicationmentioning

confidence: 99%