Examination of the Polyclonal B Cell Activator and Adjuvant from Fungi

Active site(s) of a B‐cell mitogen, vesiculogen, was investigated by means of chemical and enzymic modifications. Vesiculogen is a non‐dialyzable fraction of a hot water extract from a fungus, Peziza vesiculosa (Yadomae et al, Microbiol. Immunol. 23, 997 (1979)), and was composed of protein (~60%), carbohydrate (~30%), and a small amount of amino sugar, uronic acid, phosphate, and lipid. The mitogenicity was not affected by periodate oxidation, N‐acylation, defatting treatment, destruction of the three dimentional structure, protease digestion, nuclease digestion, N‐bromosuccinimide treatment, and β‐mercaptoethanol treatment. However, the mitogenicity was decreased by a modification of carboxyl groups, such as 1‐ethyl‐3(3‐dimethylamino propyl)‐carbodiimide or dicyclohexylcarbodiimide treatment. Vesiculogen contains a large amount of acidic amino acids. These results suggest that the mitogenicity of vesiculogen is due to the presence of anionic groups, such as aspartic acid and glutamic acid, and that the mitogenic substance is a novel, heat stable polyanionic B‐cell activator obtained from Ascomycotina.

show abstract

Distribution of the mitogenic protein, sclerogen, in extracts from Sclerotinia sclerotiorum IFO 9395 assessed by using immunochemical analysis.

Shinohara¹,

Ohno²,

Yadomae³

1991

Journal of Pharmacobio-Dynamics

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Examination of the Polyclonal B Cell Activator and Adjuvant from Fungi

Cited by 7 publications

References 14 publications

Enhancement of the resistance of tilapia and grass carp to experimental Aeromonas hydrophila and Edwardsiella tarda infections by several polysaccharides

Enhancement of the resistance of tilapia and grass carp to experimental Aeromonas hydrophila and Edwardsiella tarda infections by several polysaccharides

Requirement of Anionic Groups for the Mitogenicity of a Fungal Mitogen, Vesiculogen

Distribution of the mitogenic protein, sclerogen, in extracts from Sclerotinia sclerotiorum IFO 9395 assessed by using immunochemical analysis.

Contact Info

Product

Resources

About