Examination of White Spot Syndrome Virus (WSSV) in White Shrimp (Litopenaeus vannamei) and Tiger Prawn (Penaeus monodon) with Polymerase Chain Reaction (PCR) Method

Abstract

Shrimp is one type of fishery commodity that can be processed into fishery products. Fishery products are included in the type of food that is susceptible to pathogenic microbial contamination. One type of pathogen that can contaminate the product the main fishery in shrimp is the White Spot Syndrome Virus (WSSV). The purpose of this study is to determine the technique of checking the WSSV virus on frozen products of white shrimp (Litopenaeus vannamei) and tiger prawns (Penaeus monodon). Research carried out in December 2019 – January 2020 at the Fish Quarantine, Quality Control and Safety of Fishery Products Surabaya II. The material used in the research is 3 samples of frozen tiger prawn product and 1 sample of frozen white shrimp product. Sample extracted using the Silica Extraction Kit and detected molecularly using the method conventional Polymerase Chain Reaction (PCR). This research is observational, then the data obtained were analyzed descriptively. The results showed that all samples shrimp negative for WSSV virus.