2013
DOI: 10.1089/sur.2012.095
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Examination with Next-Generation Sequencing Technology of the Bacterial Microbiota in Bronchoalveolar Lavage Samples after Traumatic Injury

Abstract: Background: We examined the microbiota of bronchoalveolar lavage (BAL) samples with next-generation sequencing (NGS) technology to determine whether its results correlate with those of standard culture methods or affect patient outcome or both. Methods: We collected BAL samples in the surgical intensive care unit (SICU) as part of the standard of care for intubated individuals who had a Clinical Pulmonary Infection Score (CPIS) ‡ 6 points. A portion of the BAL fluid was sequenced for the 16S region of ribosoma… Show more

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Cited by 16 publications
(8 citation statements)
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“…In some cases, however, no clear pathogen can be detected by conventional culture methods11, while in other cases a significant growth of oro-pharyngeal or cutaneous commensals including Staphylococcus epidermidis can be observed, suggesting that these organisms are not as harmless as frequently believed12. Recently, the presence of oro-pharyngeal microorganisms was demonstrated on the ETTs of mechanically ventilated patients6 and in respiratory samples11, however, the presence of any of these organisms on the ETT did not always lead to VAP with these bacteria712, indicating a contribution from host factors and the general health status of the patient. In fact, an important role for the entire bacterial consortium on the ETT in the development of VAP has been suggested6 and involves interspecies interactions that provide nutrient sources or produce growth inhibiting molecules1314.…”
mentioning
confidence: 99%
“…In some cases, however, no clear pathogen can be detected by conventional culture methods11, while in other cases a significant growth of oro-pharyngeal or cutaneous commensals including Staphylococcus epidermidis can be observed, suggesting that these organisms are not as harmless as frequently believed12. Recently, the presence of oro-pharyngeal microorganisms was demonstrated on the ETTs of mechanically ventilated patients6 and in respiratory samples11, however, the presence of any of these organisms on the ETT did not always lead to VAP with these bacteria712, indicating a contribution from host factors and the general health status of the patient. In fact, an important role for the entire bacterial consortium on the ETT in the development of VAP has been suggested6 and involves interspecies interactions that provide nutrient sources or produce growth inhibiting molecules1314.…”
mentioning
confidence: 99%
“…Beneficial dietary fungi and byproducts potentially offer nutritive value, stimulate host immunity, and exert selective pressure on digestive 1 bacteria resulting in improved production (Buts and De Keyser, 2006;Gao et al, 2008;Willis et al, 2009). Probiotic and prebiotic properties of Saccharomyces spp., Aspergillus spp., and Candida spp.…”
mentioning
confidence: 99%
“…With clinical suspicion of VAP, comparisons of dominant taxa by sequencing versus organisms grown in microbial cultures of lower respiratory tract specimens had overall limited concordance [7779], and in certain cases indicted previously unsuspected organisms as VAP culprits (e.g. Dialister pneumosintes [78] or Ureaplasma parvum [76]).…”
Section: The Microbiome In Acute Respiratory Failure and Ardsmentioning
confidence: 99%