To achieve the pleasures of successful work place is an art of coping with stress. Job stress is When the level of stress increases from the resources and coping abilities possessing by someone then results can be unfavorable both for employees and organization because stress is a crucial factor influencing both performance and work in organizations. In this new era of proficiency, the job stress is a very important considerable matter at work place. That's why this research will extensively focus on the impact of work overload, role conflict and role ambiguity on job stress and further the effect of job stress on job satisfaction and also on turnover intention. This research will try to identify the causes and effect of job stress on employee's behaviors, attitudes and their willingness towards achieving goals and efficiency. A questionnaire (in which scales were consisting of twenty six items) is prepared to find out the association between role conflicts, role ambiguity, work overload and job stress and furthermore between job stress, turnover intention and job satisfaction on the basis of regression analysis. The results shows that between the variables there are very significant relationships are present. Role ambiguity, role conflict, work overload, and turnover intention have positive correlation with job stress but between job stress and job satisfaction there is negative correlation and there is also a negative correlation among job satisfaction and turnover intention. The scales and instruments are taken from the standard sources. The research results are valuable for a large number of companies, institutes and different departments to improve their productivity, to know the employees behaviors and for the welfare of both employees and organizations. For training oriented organizations research results have practical implications.