Examining the association of MMP-1 gene −1607 (2G/1G) and −519 (A/G) polymorphisms with the risk of osteomyelitis

Abstract

To investigate the effects of matrix metalloproteinase-1 (MMP-1) gene polymorphisms on the onset of osteomyelitis in Chinese Han population.In all, 80 osteomyelitis patients (case group) and 81 healthy people (control group) were recruited into this case-control study. Polymerase chain reaction-restriction fragment length polymorphism method was utilized to examine the genotypes of MMP-1 polymorphisms (−1607 2G/1G and −519A/G) in the 2 groups. Genotype and allele differences between the case and control groups were analyzed by chi-square test. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated to present the association strength between MMP-1 gene polymorphisms and osteomyelitis.Frequencies of −1607 2G/2G genotype between the case and control groups were statistically significant (P = .025). Compared with 1G/1G genotype carriers, the 2G/2G genotype carriers had 1.605 times risk of developing osteomyelitis (OR 2.605, 95% CI 1.116–6.082). Meanwhile, the 2G allele significantly associated with the risk of osteomyelitis (OR 1.735, 95% CI 1.115–2.701). In addition, frequency of −519GG genotype was obviously higher in case group than that in control group (P = .024), and GG genotype related to an increased risk of osteomyelitis (OR 2.792, 95% CI 1.127–6.917). Whereas, the −519G allele may be a susceptible factor for osteomyelitis (OR 1.622, 95% CI 1.038–2.536).The MMP-1 −1607 (2G/1G) and −519 (A/G) polymorphisms may contribute to the onset of osteomyelitis.

Genetic Determinants for Bacterial Osteomyelitis: A Focused Systematic Review of Published Literature

“…Seven studies have reported the associations between SNPs and three types of osteomyelitis (posttraumatic, hematogenous, and vascular insufficiency-related bacterial osteomyelitis; Asensi et al, 2003;Montes et al, 2006;Valle-Garay et al, 2013;Jiang et al, 2016;Hou et al, 2018;Perez-Is et al, 2019;Zhao et al, 2020). The other three studies have reported the relationship of SNP with unspecified types of bacterial osteomyelitis (Ocana et al, 2007;Tsezou et al, 2008;Kong et al, 2017). This systematic review involved 1,248 patients with bacterial osteomyelitis, including 719 patients with posttraumatic bacterial osteomyelitis, 190 patients with hematogenous bacterial osteomyelitis, 98 patients with vascular insufficiency-related bacterial osteomyelitis, and 241 patients with unspecified types of bacterial osteomyelitis.…”

“…Serum C-reactive protein (p = 0.017) and IL-6 (p = 0.006) levels were significantly higher in patients with posttraumatic osteomyelitis accompanied with the GG genotype, instead of the CG genotype. Kong et al (2017) have concluded that the G allele of rs1144393 in the MMP1 gene was regarded as a genetic risk factor and carriers of the GG genotype have an increased risk of osteomyelitis.…”

Vitamin D Receptor Genetic Polymorphisms Associate With a Decreased Susceptibility to Extremity Osteomyelitis Partly by Inhibiting Macrophage Apoptosis Through Inhibition of Excessive ROS Production via VDR-Bmi1 Signaling

“…Recently, growing evidence has shown that single nucleotide polymorphism (SNP) also plays an important role in OM development. Several SNP sites have been found to be associated with the risk of OM development, such as rs16944, rs2234663, rs1143627, rs4251961, and rs1800796 (interleukin, IL genes) ( Alves De Souza et al, 2017 ; Jiang et al, 2020b ), rs45567233 (cathepsin G, CTSG gene) ( Pérez-Is et al, 2019 ), rs1799750, and rs1144393 (matrix metalloproteinase-1, MMP-1 gene) ( Kong et al, 2017 ), implying SNPs participate in OM pathogenesis.…”

Potential novel proteomic biomarkers for diagnosis of vertebral osteomyelitis identified using an immunomics protein array technique

“…[ 8 ] CRP and ESR are currently used in all patients suspected of having VO; however, they lack the accuracy to differentiate between patients with and without VO. [ 4 ] Anti-glucosaminidase IgG, [ 9 ] iron-regulated surface determinant protein B, [ 10 ] CCL11/eotaxin, [ 11 ] interleukin-6, [ 11 ] S100 calcium binding protein A11, E-selectin, and matrix metalloproteinase-1 [ 12 ] have been reported as potential biomarkers [ 13 ] ; yet, they have not been used for the clinical diagnosis of VO. A review of the literature, employing a data mining approach using Open Targets Platform ( https://www.targetvalidation.org/ ), [ 14 ] presented a list of VO-associated antigens showing elevated autoantibody responses [ 14 ] ; the results are shown in Supplemental Digital Content (Appendix 2, which lists open target database).…”