Examining the effect of manganese on physiological processes: Invertebrate models

Pankau, Cecilia; Nadolski, Jeremy; Tanner, Hannah N.; Cryer, Carlie; Girolamo, John Di; Haddad, Christine N.; Lanning, Matthew; Miller, M.F.; Neely, Devan; Wilson, Reece; Whittinghill, BreAnna; Cooper, Robin L.

doi:10.1016/j.cbpc.2021.109209

Cited by 8 publications

(6 citation statements)

References 51 publications

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“…While adding 20 mM ZnCl 2 does increase osmolarity, changes of this degree are known to not alter PD activity or nerve conduction in the preparations. The osmolarity of crab saline is approximately 1041 Osm, and adding 1 M sucrose or 1 M mannitol to the normal crab saline did not have any effect on neural activity within the time frame used for these studies [33]. A similar phenomenon was recently reported for sensory neurons in crayfish, where an increase in nerve excitability occurred with exposure to ZnCl 2 (1 mM) [48].…”

Section: Discussionsupporting

confidence: 79%

“…The general procedures followed are similar to those previously described in detail [28,29,33] and in a video format [37].…”

Section: Methodsmentioning

confidence: 99%

“…These SACs are also not altered by the selective compounds that affect SACs of the PIEZO 1 subtype (i.e., YODA 1, JEDI 2, OB 1, and DOOKU) [31]. Recent research has used crab proprioceptive organs as a marine neuronal model to examine the effects of heavy metal exposure, as well as to investigate various concepts of neurophysiology and to pharmacologically profile SACs [32][33][34][35][36]. The purpose of this experiment was to investigate how zinc (Zn 2+ ) affects proprioceptive neural activity and nervous signal transmission.…”

Section: Introductionmentioning

confidence: 99%

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The Effects of Zinc on Proprioceptive Sensory Function and Nerve Conduction

Elliott,

Brock,

Taul

et al. 2023

NeuroSci

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Zinc (Zn2+) is an essential element that can promote proper organ function, cell growth, and immune response; it can also, however, be present in too great a quantity. Zinc toxicity caused by overexposure may result in both minor and major physiological effects, with chronic exposure at low levels and acute exposure at high levels being harmful or even toxic. This investigation examines the effects of acute exposure to relatively high concentrations of Zn2+ on sensory nerve function and nerve conduction. A proprioceptive nerve in marine crab (Callinectes sapidus) limbs was used as a model to assess the effects of Zn2+ on stretch-activated channels (SACs) and evoked nerve conduction. Exposure to Zn2+ slowed nerve condition rapidly; however, several minutes were required before the SACs in sensory endings were affected. A depression in conduction speed and an increase followed by a decrease in amplitude were observed for the evoked compound action potential, while the frequency of nerve activity upon joint movement and stretching of the chordotonal organ significantly decreased. These altered responses could be partially reversed via extensive flushing with fresh saline to remove the zinc. This indicates that subtle, long-term exposure to Zn2+ may alter an organism’s SAC function for channels related to proprioception and nerve conduction.

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Section: Discussionsupporting

confidence: 79%

“…The general procedures followed are similar to those previously described in detail [28,29,33] and in a video format [37].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Effects of Zinc on Proprioceptive Sensory Function and Nerve Conduction

Elliott,

Brock,

Taul

et al. 2023

NeuroSci

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“…At the physiological level, several biochemical markers, including tyrosine hydroxylase activity, dopamine levels, and mitochondrial activity, are affected ( Mohandas et al, 2017 ; Silva et al, 2021 ). A recent study identified acute effects of Mn toxicity, including altered neurotransmission at the Drosophila neuromuscular junction (a glutamate synapse) and abnormal cardiac function ( Pankau et al, 2022 ). Together, these outcomes identify Drosophila as a useful model organism to study Mn toxicity.…”

Section: Introductionmentioning

confidence: 99%

Divalent metal content in diet affects severity of manganese toxicity in Drosophila

Ghosn,

Sparks,

Spaulding

et al. 2024

Biology Open

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Dysregulation of manganese (Mn) homeostasis is a contributing factor in many neuro-degenerative diseases. Adult Drosophila are sensitive to excessive levels of dietary Mn, dying relatively early, and exhibiting biochemical and mobility changes reminiscent of Parkinsonian conditions. To further study Mn homeostasis in Drosophila, we sought to test lower levels of dietary Mn (5 mM) and noted a striking difference in Canton-S adult survivorship on different food. On a cornmeal diet, Mn-treated flies live only about half as long as untreated siblings. Yet, with the same Mn concentration in a molasses diet, adults survive about 80% as long as untreated siblings, and adults raised on a sucrose-yeast diet are completely insensitive to this low dose of dietary Mn. By manipulating metal ion content in the cornmeal diet, and measuring the metal content in each diet, we traced the difference in life span to the levels of calcium and magnesium in the food, suggesting that these ions are involved in Mn uptake and/or utilization. Based on these findings, it is recommended that the total dietary load of metal ions be considered when assessing Mn toxicity.

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“…The SACs are not altered by traditional agonists or antagonists like amiloride, ruthenium red, or streptomycin [59,60]; they are also not altered by selective compounds for PIEZO 1 subtype SACs (i.e., YODA 1, JEDI 2, OB 1, and DOOKU) [60]. Crab proprioceptive organs are being used as neuronal models for marine species to address the effects of heavy metal exposure, concepts of neurophysiology, and pharmacological profiling of SACs [61][62][63][64][65].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Lithium on Proprioceptive Sensory Function and Nerve Conduction

Brock,

Elliott,

Taul

et al. 2023

NeuroSci

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Animals are exposed to lithium (Li+) in the natural environment as well as by contact with industrial sources and therapeutic treatments. Low levels of exposure over time and high volumes of acute levels can be harmful and even toxic. The following study examines the effect of high-volume acute levels of Li+ on sensory nerve function and nerve conduction. A proprioceptive nerve in the limbs of a marine crab (Callinectes sapidus) was used as a model to address the effects on stretch-activated channels (SACs) and evoked nerve conduction. The substitution of Li+ for Na+ in the bathing saline slowed nerve conduction rapidly; however, several minutes were required before the SACs in sensory endings were affected. The evoked compound action potential slowed in conduction and slightly decreased in amplitude, while the frequency of nerve activity with joint movement and chordotonal organ stretching significantly decreased. Both altered responses could be partially restored with the return of a Na+-containing saline. Long-term exposure to Li+ may alter the function of SACs in organisms related to proprioception and nerve conduction, but it remains to be investigated.

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Examining the effect of manganese on physiological processes: Invertebrate models

Cited by 8 publications

References 51 publications

The Effects of Zinc on Proprioceptive Sensory Function and Nerve Conduction

The Effects of Zinc on Proprioceptive Sensory Function and Nerve Conduction

Divalent metal content in diet affects severity of manganese toxicity in Drosophila

The Effects of Lithium on Proprioceptive Sensory Function and Nerve Conduction

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