Examining the Effects of Temperature on Recombination in Wheat

Coulton, Alexander; Burridge, Amanda J.; Edwards, Keith J.

doi:10.3389/fpls.2020.00230

Cited by 19 publications

(11 citation statements)

References 34 publications

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“…Lloyd et al proposed that MR rate of Arabidopsis under different temperature conditions within fertility-tolerable range displays a 'U-shape' pattern, in which both decreased (8°C) and increased (28°C) temperatures promote MR rate (Lloyd et al, 2018). A similar influential pattern was found in wheat incubated between 10~26°C (Coulton et al, 2020), which suggests that the positive impact of higher temperatures within fertility threshold on MR frequency may be conserved between dicots and monocots. Under a higher temperature at 32°C, Arabidopsis plants produce univalents indicating for a lowered MR frequency (De Storme and Geelen, 2020).…”

Section: Discussionmentioning

confidence: 90%

Heat Stress Interferes with Formation of Double-Strand Breaks and Homolog Synapsis inArabidopsis thaliana

Ning

Liu

Wang

et al. 2020

Preprint

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Meiotic recombination (MR) drives reciprocal exchange of genetic information which allows novel combination of alleles and contributes to genomic diversity of eukaryotes. In this study, we report that heat stress (36-38°C) over fertile threshold fully abolishes crossover occurrence by lowering the formation of SPO11-dependent double-strand breaks (DSBs) in Arabidopsis. The high temperature partially suppresses chromosome fragmentation in the rad51 and syn1 mutants by reducing DSB formation. In addition, we found that although the SYN1-mediated formation of chromosome axis is not influenced by the high temperature, ASY1-associated axial element of synaptonemal complex (SC) is partially compromised, and the ZYP1-dependent central element of axis is severely disrupted, indicating that homology synapsis is another prominent target of high temperature. Besides, we observed that the loading of recombinase DMC1 on chromatin is significantly reduced by high temperatures, suggesting that heat stress interferes with DMC1-dependent MR. Moreover, we found that the numbers of DSB and DMC1 keep stable within a limited range of temperature increase, while decrease when the DSB-processing system reaches threshold to heat. Furthermore, transcriptome study revealed a universal variation of the expression of meiotic genes under low (4°C) and high (32°C) temperatures, suggesting that temperature may influence MR by modulating the transcription of MR-related factors. We also found that the landscape of 5-methylcytosine (5mC) was altered under high temperatures, suggesting that environmental temperature may influence the distribution of MR via reshaped DNA methylation status of chromatin. Taken together, our findings provide evidence shedding light on how environment temperatures influence MR in Arabidopsis.

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Section: Discussionmentioning

confidence: 90%

Heat Stress Interferes with Formation of Double-Strand Breaks and Homolog Synapsis inArabidopsis thaliana

Ning

Liu

Wang

et al. 2020

Preprint

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“…The change of temperature reduces the polarization of axis formation, and ASY1 is detected more evenly on the chromosomes which is associated with an elevation of interstitial and centromere-proximal chiasmata ( Higgins et al, 2012 ). However, this strategy may not be applicable to every crops, as seen in the observation that wheat recombination is only slightly and locally altered at high temperature ( Coulton et al, 2020 ).…”

Section: Engineering Meiotic Recombinationmentioning

confidence: 99%

Rewiring Meiosis for Crop Improvement

2021

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Meiosis is a specialized cell division that contributes to halve the genome content and reshuffle allelic combinations between generations in sexually reproducing eukaryotes. During meiosis, a large number of programmed DNA double-strand breaks (DSBs) are formed throughout the genome. Repair of meiotic DSBs facilitates the pairing of homologs and forms crossovers which are the reciprocal exchange of genetic information between chromosomes. Meiotic recombination also influences centromere organization and is essential for proper chromosome segregation. Accordingly, meiotic recombination drives genome evolution and is a powerful tool for breeders to create new varieties important to food security. Modifying meiotic recombination has the potential to accelerate plant breeding but it can also have detrimental effects on plant performance by breaking beneficial genetic linkages. Therefore, it is essential to gain a better understanding of these processes in order to develop novel strategies to facilitate plant breeding. Recent progress in targeted recombination technologies, chromosome engineering, and an increasing knowledge in the control of meiotic chromosome segregation has significantly increased our ability to manipulate meiosis. In this review, we summarize the latest findings and technologies on meiosis in plants. We also highlight recent attempts and future directions to manipulate crossover events and control the meiotic division process in a breeding perspective.

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“…Environment is thus certainly very different between all the origins of our landraces and temperature should vary a lot in each location and is not stable enough to affect durably and maintain a different recombination profile between the four populations. Moreover, it was recently shown that increased temperature up to 28°C for three weeks during wheat meiosis has only a limited impact on recombination distribution (Coulton et al 2020).…”

Section: Evolution Of the Recombination Landscape In Bread Wheatmentioning

confidence: 99%

Evolution of recombination landscapes in diverging populations of bread wheat

Déserts

Bouchet

Sourdille

et al. 2021

Preprint

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Reciprocal exchanges of DNA (crossovers) that occur during meiosis are mandatory to ensure the production of fertile gametes in sexually reproducing species. They also contribute to shuffle parental alleles into new combinations thereby fuelling genetic variation and evolution. However, due to biological constraints, the recombination landscape is highly heterogenous along the genome which limits the range of allelic combinations and the adaptability of populations. An approach to better understand the constraints on the recombination process is to study how it evolved in the past. In this work we tackled this question by constructing recombination profiles in four diverging bread wheat (Triticum aestivum L.) populations established from 371 landraces genotyped at 200,062 SNPs. We used linkage disequilibrium (LD) patterns to estimate in each population the past distribution of recombination along the genome and characterize its fine-scale heterogeneity. At the megabase scale, recombination rates derived from LD patterns were consistent with family-based estimates obtained from a population of 406 recombinant inbred lines. Among the four populations, recombination landscapes were significantly positively correlated between each other and shared a statistically significant proportion of highly recombinant intervals. However, this comparison also highlighted that the similarity in recombination landscapes between populations was significantly decreasing with their genetic differentiation in most regions of the genome. This observation was found to be robust to SNP ascertainment and demography and suggests a relatively rapid evolution of factors determining the fine-scale localization of recombination in bread wheat.

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Examining the Effects of Temperature on Recombination in Wheat

Cited by 19 publications

References 34 publications

Heat Stress Interferes with Formation of Double-Strand Breaks and Homolog Synapsis inArabidopsis thaliana

Heat Stress Interferes with Formation of Double-Strand Breaks and Homolog Synapsis inArabidopsis thaliana

Rewiring Meiosis for Crop Improvement

Evolution of recombination landscapes in diverging populations of bread wheat

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