Several gender differences exist in the transport sector. These include accessibility to transport modes, safety and security when travelling, and the participation of women in transport research and innovation (R&I). In order to achieve sustainable and inclusive transport, planners and policymakers should consider all impacts on gender equality. This paper sheds light on two main issues which interconnect through the decision-making process. The first relates to women’s behaviour in the transport system (i.e., studies the gender mobility gap). The second concerns the role of women in transport R&I, particularly the topics covered by research projects and relevant descriptive statistics of their participation in the sector. Based on a literature review, this paper identifies critical issues in the European transport sector and key European Union policy initiatives and regulations that address gender equality and transport. The European Commission’s Transport Research and Innovation Monitoring and Information System (TRIMIS) is used to summarise the status and evolution of European research in addressing women’s issues in transport. It also analyses the participation of women in European transport research and innovation activities. The paper assesses progress to date and identifies challenges and opportunities for women, mobility, and transport. It concludes by providing policy recommendations to overcome the major barriers to gender equality in the European transport sector and to transport research and innovation.