Examining the occurrence of residues of flunixin meglumine in cull dairy cows by use of the flunixin cull cow survey

Abstract

Objective—To determine whether cull dairy cows with signs of certain clinical conditions, termed suspect, are more likely than healthy-appearing cull dairy cows to have violative concentrations of flunixin meglumine in their tissues at slaughter. Design—Cross-sectional study. Animals—961 cull dairy cows. Procedures—Suspect cull dairy cows were selected from 21 beef slaughter establishments with a high production volume of dairy cows, and kidney and liver tissues were collected for screening. Kidney tissues were screened for antibiotics and sulfonamides with the fast antimicrobial screening test (FAST). Liver tissues were screened for flunixin meglumine with an ELISA, and quantitative analysis of ELISA-positive samples was performed with high-performance liquid chromatography. During the same time period, liver tissues from 251 healthy-appearing cull dairy cows were collected for the Food Safety and Inspection Service National Residue Program Scheduled Sampling Plan, but were screened only for flunixin meglumine. Results—Of 710 suspect cull dairy cows, 50 (7.04%) had liver tissue flunixin concentrations higher than the flunixin tolerance concentration (0.125 ppm). Thirty-one of 168 (18.45%) FAST-positive and 19 of 542 (3.51%) FAST-negative suspect cull dairy cows had violative tissue flunixin concentrations. Two of the 251 (0.80%) healthy-appearing cull dairy cows had violative tissue flunixin concentrations. Conclusions and Clinical Relevance—Suspect cull dairy cows, especially those that were also FAST positive, had a significantly higher incidence of violative tissue flunixin concentrations than healthy-appearing cull dairy cows at slaughter. Targeted sampling plans for flunixin meglumine in suspect dairy cows can help to support more efficient use of resources and further safeguard the nation's food supply.

Distribution of Flunixin Residues in Muscles of Dairy Cattle Dosed with Lipopolysaccharide or Saline and Treated with Flunixin by Intravenous or Intramuscular Injection

“…For flunixin, violations in dairy cows seem to be associated with "suspect" animals, those animals "having clinical signs or evidence of disease or conditions that may result in condemnation of the entire carcass" such as lameness, mastitis, metritis, and lesions associated with injections. 15 The current study generally supports the regulatory framework in place that emphasizes testing target tissues of suspect animals. Our data show small but significant differences between muscle types, indicating the muscle used for survey should be specified.…”

Role of ABCG2 in Secretion into Milk of the Anti-Inflammatory Flunixin and Its Main Metabolite: In Vitro-In Vivo Correlation in Mice and Cows

“…Several pharmacokinetics and residue research studies and surveys have been previously undertaken with FLU and 5OH-FLU on different farm species such as dairy cows (Jedziniak et al, 2009(Jedziniak et al, , 2013Deyrup et al, 2012;Kissell et al, 2012Kissell et al, , 2013Kissell et al, , 2015, sheep (Marini et al, 2016), and goats (Königsson et al, 2003). However, this is the first study to explore the in vivo role of the ABCG2 transporter in these processes for an anti-inflammatory drug in farm animals.…”

A Framework for Meta-Analysis of Veterinary Drug Pharmacokinetic Data Using Mixed Effect Modeling

“…For the perspective of violative residues, physiological states can also be an important factor. According to previous research, the suspect cull cows have a significant higher possibility of violative flunixin concentrations than healthy dairy cows . Mastitis is another disease that has a great effect on antibiotic residues in milk.…”

“…According to previous research, the suspect cull cows have a significant higher possibility of violative flunixin concentrations than healthy dairy cows. 70 Mastitis is another disease that has a great effect on antibiotic residues in milk. Administered veterinary drugs are inclined to persist longer in milk from mastitic cows.…”