Examining the phylogeny of the Australasian Lymnaeidae (Heterobranchia: Pulmonata: Gastropoda) using mitochondrial, nuclear and morphological markers

Phylogenetic relationships and evolution of pulmonate gastropods (Mollusca): New insights from increased taxon sampling

“…The monophyly of Physidae (e.g., Wethington and Lydeard, 2007), Lymnaeidae (e.g., Remigio and Blair, 1997;Puslednik et al, 2009), Acroloxidae (e.g., Walther et al, 2006) is recovered here with the highest support. However, new markers are needed to determine the deep relationships among the major clades of freshwater pulmonates.…”

Taxonomic status of Stagnicola palustris (O. F. Müller, 1774) and S. turricula (Held, 1836) (Gastropoda: Pulmonata: Lymnaeidae) in view of new molecular and chorological data

“…In our analyses we used molecular markers frequently applied for taxonomic or phylogenetic studies, i.e. sequences of mitochondrial 3'COI (loaiza et al 2013, zeineDDine et al 2014) and 5'COI (RemiGio & hebeRt 2003, runDell et al 2004, Perez et al 2005, kane et al 2008, Falniowski et al 2009, Falniowski & szaRowska 2011, szaRowska et al 2013 gene fragments as well as nuclear ITS2 fragment of rDNA gene (baRGues & mas-coma 2005, källeRsJö et al 2005, Puslednik et al 2009, baRGues et al 2012, loaiza et al 2013). The nucleotide sequences of mitochondrial 5'COI gene ("barcode sequence") differentiate between the above three lymnaeids at the level of 14.0-20.8%.…”

“…Therefore we tried to use also characters used in classical taxonomy which is often practiced by other researchers (e.g. szaRowska , haase et al 2007, Falniowski et al 2009, Puslednik et al 2009, Falniowski & szaRowska 2011, szaRowska et al 2013. Each specimen taken for molecular studies was identified on the basis of its genital anatomy (according to and GlöeR & meieR-bRook 2003.…”

“…Since the 1990s several different molecular methods have been intensively used to solve taxonomic problems among Lymnaeidae (RemiGio & Blair 1997a, b, baRGues & mas-coma 1997, baRGues et al 1997, RemiGio 2002, stothaRd et al 2000, tRouve et al 2000, Rybska et al 2000a, knott et al 2003, RemiGio & hebeRt 2003, caRvalho et al 2004, De Boer et al 2004, PFenninGeR et al 2006, Puslednik et al 2009, coRRea et al 2010, vinaRski 2013. As a result, some changes have been also introduced in the above classification of the genus Stagnicola.…”

New records of non-marine, non-camaenid gastropods (Mollusca: Gastropoda) from islands off the Kimberley coast, Western Australia

“…According to the currently accepted taxonomy, two species of Austropeplea are recognised from Australian freshwater habitats, A. lessoni and A. tomentosa (ABRS 2009;Puslednik et al, 2009 …”