Professor, Department of Physics 34 Florida International University, AHC4 310 35 Modesto Maidique Campus 36 11200 SW 8 th Street 37 Miami, FL 33199 38 305.348.6737 (phone) 39 305.348.6700 (fax) 40 alaird@fiu.edu 41 42 43 † These authors contributed equally to this work. 44 45 46 1 ABSTRACT 47 48Anxiety is known to dysregulate the salience, default mode, and central executive networks of the human 49 brain, yet this phenomenon has not been fully explored across the STEM learning experience, where 50 anxiety can impact negatively academic performance. Here, we evaluated anxiety and large-scale brain 51 connectivity in 101 undergraduate physics students. We found sex differences in STEM-related and clinical 52 anxiety, with longitudinal increases in science anxiety observed for both female and male students. Sex-53 specific relationships between STEM anxiety and brain connectivity emerged, with male students 54 exhibiting distinct inter-network connectivity for STEM and clinical anxiety and female students 55 demonstrating no significant within-sex correlations. Anxiety was negatively correlated with academic 56 performance in sex-specific ways at both pre-and post-instruction. Moreover, math anxiety in male 57 students mediated the relation between default mode-salience connectivity and course grade. Together, 58 these results reveal complex sex differences in the neural mechanisms driving how anxiety is related to 59 STEM learning . 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 2 Today's universities and colleges are tasked with the challenge of developing novel strategies for 94 improving undergraduate academic performance and ensuring that students are prepared for successful 95 careers. In particular, emphasis is placed on enhancing student outcomes and generating enthusiasm for 96 the science, technology, engineering, and mathematics (STEM) disciplines. However, STEM students 97 encounter multiple, major-specific challenges, including intensive laboratory, project-based, and lecture-98 based coursework (Thiry et al., 2011), heightened classroom competition (Strenta et al., 1994 Gasiewski 99 et al., 2012), and academic challenges of STEM courses (Strenta et al., 1994; Rask, 2010). As such, many 100 students often struggle with STEM-related anxiety, which manifests as an unease, avoidance, or fear of 101 learning science or math topics. In particular, female STEM students, relative to their male counterparts, 102 are disproportionately affected by higher rates of STEM anxiety (Mallow, 1994; Brownlow et al., 2000; 103 Baloglu and Kocak, 2006; Mallow et al., 2010). This may be due to STEM-related barriers that adversely 104 impact achievement and performance (Kiefer and Sekaquaptewa, 2007; Nosek et al., 2009), including 105 stereotype threat (Shapiro and Williams, 2012), gender-based bias (Moss-Racusin et al., 2012), and lack of 106 non-stereotypical role models (Cheryan et al., 2011; Hernandez et al., 2018).
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