Excavating differentially expressed antimicrobial peptides from transcriptome of Larimichthys crocea liver in response to Cryptocaryon irritans

Zheng, Libing; Mao, Yong; Wang, Jun; Chen, Ruan-Ni; Su, Yongquan; Yang, Hong; Hong, Yu-jian; Hong, Yu-cong

doi:10.1016/j.fsi.2018.01.028

Cited by 21 publications

(5 citation statements)

References 51 publications

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“…Regarding AMPs, few available observations indicate that hepcidin, NK-lysin (NKL), and lysozymes seem to be involved in the immune response against parasites. Thus, the transcription of the gene that codes for hepcidin, named hamp, is up-regulated in several fish species upon parasite infection, including C. irritans infection [17][18][19][20]. Moreover, NKL, a well-known AMP produced by cytotoxic T-lymphocytes (CTL), has shown antiparasitic effects in turbot (Scophthalmus maximus) infected with the ciliate Philasterides dicentrarchi, and in yellow croaker (Larimichthys crocea) specimens infected with C. irritans [21,22].…”

Section: Introductionmentioning

confidence: 99%

Severe Natural Outbreak of Cryptocaryon irritans in Gilthead Seabream Produces Leukocyte Mobilization and Innate Immunity at the Gill Tissue

Cervera

González-Fernández

Arizcún

et al. 2022

IJMS

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The protozoan parasite Cryptocaryon irritans causes marine white spot disease in a wide range of fish hosts, including gilthead seabream, a very sensitive species with great economic importance in the Mediterranean area. Thus, we aimed to evaluate the immunity of gilthead seabream after a severe natural outbreak of C. irritans. Morphological alterations and immune cell appearance in the gills were studied by light microscopy and immunohistochemical staining. The expression of several immune-related genes in the gills and head kidney were studied by qPCR, including inflammatory and immune cell markers, antimicrobial peptides (AMP), and cell-mediated cytotoxicity (CMC) molecules. Serum humoral innate immune activities were also assayed. Fish mortality reached 100% 8 days after the appearance of the C. irritans episode. Gill filaments were engrossed and packed without any space between filaments and included parasites and large numbers of undifferentiated and immune cells, namely acidophilic granulocytes. Our data suggest leukocyte mobilization from the head kidney, while the gills show the up-regulated transcription of inflammatory, AMPs, and CMC-related molecules. Meanwhile, only serum bactericidal activity was increased upon infection. A potent local innate immune response in the gills, probably orchestrated by AMPs and CMC, is triggered by a severe natural outbreak of C. irritans.

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Section: Introductionmentioning

confidence: 99%

Severe Natural Outbreak of Cryptocaryon irritans in Gilthead Seabream Produces Leukocyte Mobilization and Innate Immunity at the Gill Tissue

Cervera

González-Fernández

Arizcún

et al. 2022

IJMS

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“…Aley et al reported that AMPs produced by specialized epithelial cells, especially the α‐defensins cryptdin‐2 and cryptdin‐3, reduce Giardia viability in vitro (Aley et al., 1994). In a previous investigation of large yellow croaker, an array of AMPs, including LEAP2‐like, LEAP‐2A, hepcidin, hepcidin‐like, piscidin‐5‐like, piscidin‐5‐like type 4 and bactericidal permeability‐increasing protein, were identified from transcriptome data and significantly upregulated after C. irritans infection (Zheng et al., 2018). However, in the gill of N. albiflora, only the apolipoprotein M, class III piscidin 7 and histone H1‐like genes were significantly changed, and the latter two were downregulated rather than upregulated after infection.…”

Section: Discussionmentioning

confidence: 99%

“…In large yellow croaker, the activities and levels of lysozyme, alkaline phosphatase, IgM, complement C3 and other immunological factors in skin mucus increase during C. irritans exposure (Yin et al., 2015). The expression of various antimicrobial peptide (AMP) genes, including LEAP2‐like, LEAP‐2A, hepcidin, hepcidin‐like, piscidin‐5‐like, piscidin‐5‐like type 4 and bactericidal permeability‐increasing protein, is increased in large yellow croaker livers after infection (Zheng et al., 2018). Cryptocaryon irritans infection also increased mucosal lysozyme activity in skin and gill, increased plasma cortisol and glucose contents, accelerated the respiratory rate, inhibited ingestion in marbled rockfish and caused gill tissue necrosis and granular hyperplasia in orange‐spotted grouper (Yin, 2014).…”

Section: Introductionmentioning

confidence: 99%

The gill transcriptome reveals unique antimicrobial features that protect Nibeaalbiflora from Cryptocaryonirritans infection

Xie

Jiang

Miao

et al. 2021

Journal of Fish Diseases

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Cryptocaryonosis is the greatest threat to most teleost species among all parasitic diseases, causing mass loss to the marine aquaculture industry. Epidemiological investigation of teleost susceptibility to Cryptocaryon irritans infection revealed that yellow drum (Nibea albiflora) is highly resistant. In order to further understand the activation of the immune system in the gill, which is one of the main mucosal‐associated lymphoid tissues and a target of parasites, transcriptome analysis of the yellow drum gill was performed. Gill samples were collected from fish challenged after 24 hr and 72 hr with theronts at a median death rate (2050 theronts per gram fish). Gene expression profiles showed that TLR5 was the only receptor that activated the downstream immune response. The infection activated complement cascade through alternative pathway and increased the expression of C5a anaphylatoxin chemotactic receptor 1. In addition, possible antimicrobial molecules, including lipoprotein and haptoglobin, which are responsible for trypanolysis in humans, were among the top significantly upregulated genes at 24 hr. After 72 hr, the expression of secreted immunoglobulin T‐related genes was induced. These results suggested a rapid innate and adaptive immune response at the mucosal level. In conclusion, the results provide new perspectives on mucosal immune resistance in yellow drum against cryptocaryonosis and provide the possibility of mining resistance genes for future therapy.

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“…In contrast, most fish species have two or even more homologous hepcidin genes [ 14 , 15 ]. Zebrafish ( Danio rerio ) and rainbow trout ( Oncorhynchus mykiss ) have two homologous hepcidin genes [ 16 , 17 ], and yellow croaker ( Larimichthys crocea ) has more [ 18 ]. In general, fish hepcidin genes can be classified into two types: hamp type-1 and hamp type-2 [ 15 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Hamp Type-1 Promotes Antimicrobial Defense via Direct Microbial Killing and Regulating Iron Metabolism in Grass Carp (Ctenopharyngodon idella)

Kurobe

Liu

et al. 2020

Biomolecules

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Hepcidin is an antimicrobial peptide and regulator of iron homeostasis which has two isoforms in most fishes and some mammals. Previous studies have reported that the two hepcidin isoforms have different roles. Hamp type-1 plays a regulatory role in iron metabolism and hamp type-2 mostly performs an antimicrobial role. In this study, we found that Ctenopharyngodon idella (C. idella) have only one hepcidin isoform (hamp type-1), which showed both broad-spectrum antibacterial and iron regulatory functions. C. idella hepcidin mature peptide (hepcidin-25) and truncated peptide (hepcidin-20) exhibited bactericidal activities against both Gram-positive and Gram-negative bacteria in a dose-dependent manner in part through membrane rupture and binding to bacterial genomic DNA. The data from challenge tests demonstrated that the administration of hepcidin-25 significantly reduced mortality rates of C. idella by A. hydrophila infection, probably due to direct bactericidal activities of the peptide and a reduction of iron content in the fish serum. In addition, a comparison between hepcidin-20 and -25 suggests that the N terminal 5 amino acids play a critical role in reducing iron content in fish serum. Our findings revealed an important role of hamp type-1 in maintaining iron homeostasis and fighting against bacterial infections, suggesting the hepcidin has implications for the prevention and control of bacterial infection in aquaculture.

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Excavating differentially expressed antimicrobial peptides from transcriptome of Larimichthys crocea liver in response to Cryptocaryon irritans

Cited by 21 publications

References 51 publications

Severe Natural Outbreak of Cryptocaryon irritans in Gilthead Seabream Produces Leukocyte Mobilization and Innate Immunity at the Gill Tissue

Severe Natural Outbreak of Cryptocaryon irritans in Gilthead Seabream Produces Leukocyte Mobilization and Innate Immunity at the Gill Tissue

The gill transcriptome reveals unique antimicrobial features that protect Nibeaalbiflora from Cryptocaryonirritans infection

Hamp Type-1 Promotes Antimicrobial Defense via Direct Microbial Killing and Regulating Iron Metabolism in Grass Carp (Ctenopharyngodon idella)

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