Excellent performance separation of trypsin by novel ternary magnetic composite adsorbent based on betaine-urea- glycerol natural deep eutectic solvent modified MnFe2O4-MWCNTs

Biomass-derived magnetic nanocomposites modified by choline chloride/citric acid based natural deep eutectic solvents for the magnetic solid phase extraction of trypsin

“…Trypsin (Tryp) is a serine protease which plays an important role in the selective hydrolysis of the peptide bonds of arginine and lysine. 1,2 In addition, Tryp can not only assist the digestion of food in organisms, but can also be used for zymogen activation, drug therapy and so on. 3,4 The traditional purification method of Tryp usually involves ammonium sulfate precipitation to crudely precipitate the target protein, and then further purify it by methods such as affinity chromatography, gel chromatography or ion exchange resin.…”

Deep eutectic solvents as an emerging green platform for the synthesis of functional materials

“…28d). Afterwards, Chen et al 215 synthesized magnetic composite adsorbents using a ternary deep eutectic solvent (DES) composed of betaine, urea, and glycerol and carbon nanotubes (CNTs) (MnFe 2 O 4 -MWCNTs@B-U-G) to extract trypsin (Fig. 28e).…”

Humic acid-based biomass-derived magnetic nanocomposites modified by a deep eutectic solvent for the efficient adsorption of trypsin

“…25 In addition, the DES structure contains abundant hydrogen bonds and plentiful biocompatibility, resulting in a wide range of applications in protein separation and purification. 26,27 For example, Chen et al 10 designed and synthesized the novel ternary magnetic nanocomposites (MnFe 2 O 4 -MWCNTs@B-U-G) by integrating the superior physicochemical properties of DES, multi-walled carbon nanotubes and MnFe 2 O 4 . Therefore, based on the unique structural designability of DES, it is often used as a surface modification material for extractants, which are widely used in the field of separation.…”

“…Therefore, based on the unique structural designability of DES, it is often used as a surface modification material for extractants, which are widely used in the field of separation. 1,10 Herein, combining the superior properties of biocompatibility, multifunctionality of HA and structural designability of DES, a novel adsorbent was designed and synthesized. Then, combined with the MSPE technology, the novel biomassderived magnetic nanocomposite was ultimately used for the efficient separation of Tryp.…”