Excellent ratiometric two-photon fluorescent probes for hydrogen sulfide detection based on the fluorescence resonance energy transfer mechanism

Ma, Xue-Xue; Geng, Ming-Hui; Cheng, Xia-Yu; Zhang, Tong-Shu; Li, Zong-Liang; Zhao, Ke

doi:10.1039/d3cp05329f

Phys. Chem. Chem. Phys.

2024

DOI: 10.1039/d3cp05329f

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Excellent ratiometric two-photon fluorescent probes for hydrogen sulfide detection based on the fluorescence resonance energy transfer mechanism

Xue-Xue Ma,

Ming-Hui Geng,

Xia-Yu Cheng

et al.

Abstract: Fluorescence resonance energy transfer (FRET) is an important mechanism to design ratiometric fluorescent probes that are favourable to detect analyte quantitatively according to the ratio of two well-resolved emission signals....

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Synthesis of a novel water-soluble pyridine dicarboxylate and its application in fluorescence cell imaging

Wei,

Qi,

et al. 2024

Heterocyclic Communications

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A novel water-soluble substituted pyridine dicarboxylate containing a quaternary ammonium ion with potential applications in fluorescence cell imaging was synthesized and characterized. Remarkably, this compound exhibited water solubility in the absence of additional solubilizers, enabling direct dissolution in phosphate-buffered saline for cell studies. No obvious cytotoxicity was observed in 4T1 cells (mouse breast cancer cells) within the concentration range of 0.2–25 μmol L−1, with a cell survival rate above 89%. Furthermore, the compound exhibited a maximum two-photon absorption cross-section of 86 GM at an excitation wavelength of 780 nm, demonstrating high cell permeability, effective distribution in the cytoplasm, and preferential targeting of organelles. Single- and two-photon fluorescence imaging of cells revealed a distinctive blue emission and strong bright green emission, respectively. The newly synthesized substituted pyridine dicarboxylate exhibited low cytotoxicity and strong fluorescence imaging properties, demonstrating its potential in cell imaging applications.

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Synthesis of a novel water-soluble pyridine dicarboxylate and its application in fluorescence cell imaging

Wei,

Qi,

et al. 2024

Heterocyclic Communications

View full text Add to dashboard Cite

show abstract

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Excellent ratiometric two-photon fluorescent probes for hydrogen sulfide detection based on the fluorescence resonance energy transfer mechanism

Abstract: Fluorescence resonance energy transfer (FRET) is an important mechanism to design ratiometric fluorescent probes that are favourable to detect analyte quantitatively according to the ratio of two well-resolved emission signals....

Cited by 1 publication

References 78 publications

Synthesis of a novel water-soluble pyridine dicarboxylate and its application in fluorescence cell imaging

Synthesis of a novel water-soluble pyridine dicarboxylate and its application in fluorescence cell imaging

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