Excellent reproducibility of laser speckle contrast imaging to assess skin microvascular reactivity

Roustit, Matthieu; Millet, C.; Blaise, S.; Dufournet, Boris; Cracowski, Jean‐Luc

doi:10.1016/j.mvr.2010.05.012

Cited by 249 publications

(224 citation statements)

References 17 publications

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“…LDF has the advantage of having a well-established theory ( [12]). However, it is a single-point monitoring technique with low reproducibility ( [13], [14]). Based on the LDF principle, laser Doppler imagers have been proposed to obtain 2D perfusion images but they rely on a scan of the zone under study (see, e.g., [15], [16]).…”

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confidence: 99%

Analysis of Laser Speckle Contrast Images Variability Using a Novel Empirical Mode Decomposition: Comparison of Results With Laser Doppler Flowmetry Signals Variability

Humeau-Heurtier

Ábrahám

2015

IEEE Trans. Med. Imaging

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noise (CEEMDAN). CEEMDAN is suitable for non linear and non stationary data and leads to intrinsic mode functions (IMFs). It is based on the ensemble empirical mode decomposition (EEMD) which relies on empirical mode decomposition (EMD). In our work the average frequencies of LSCI IMFsgiven by CEEMDAN are compared with the ones given by EMD and EEMD. Moreover, LDF signals acquired simultaneously to LSCI data are also processed with CEEMDAN, EMD and EEMD. We show that the average frequencies of IMFs given by CEEMDAN depend on the signal to noise ratio (SNR) used in the computation but, for a given SNR, the average frequencies found for LSCI are close to the ones obtained for LDF. By opposition, EEMD leads to IMFs with frequencies that do not vary much when the SNR level is higher than a threshold. The new CEEMDAN algorithm has the advantage of achieving a complete decomposition with no error in the reconstruction but our study suggests that further work is needed to gain knowledge in the adjustment of the added noise level. CEEMDAN, EMD and EEMD are data-driven methods that can provide a better knowledge of LSCI.Index Terms-Laser speckle contrast imaging, Empirical mode decomposition, Biomedical image processing, Laser Doppler flowmetry, Microvascular blood flow.

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confidence: 99%

Analysis of Laser Speckle Contrast Images Variability Using a Novel Empirical Mode Decomposition: Comparison of Results With Laser Doppler Flowmetry Signals Variability

Humeau-Heurtier

Ábrahám

2015

IEEE Trans. Med. Imaging

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“…LSCI is able to scan a larger area of skin (typically 15 x 15 cm) in a time frame of a few seconds while at the same time maintaining a high spatial resolution (0.2 mm/pixel at 25 cm distance). The technique has been shown to have a better reproducibility than LDF (Puissant et al, 2013;Roustit et al, 2010), and this has been attributed to the fact that blood flow changes can be assessed in an area instead of in a single point.…”

Section: Laser Speckle Contrast Imagingmentioning

confidence: 99%

Assessment of microvascular and metabolic responses in the skin

Iredahl¹

2016

Linköping University Medical Dissertations

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The general aim of this project was to develop experimental in vivo models that allow for minimally invasive investigations of responses in the skin to microvascular and metabolic provocations. The cutaneous microvasculature has emerged as a valuable model and been proposed to mirror the microcirculation in other organs. Dysfunction in the cutaneous microcirculation has thus been linked to systemic diseases such as hypertension and diabetes mellitus. Models for investigating skin responses could facilitate the understanding of pathophysiological mechanisms as well as effects of drugs. In the first study, three optical measurement techniques (laser Doppler flowmetry (LDF), laser speckle contrast imaging (LSCI) and tissue viability imaging (TiVi)) were compared against each other and showed differences in their ability to detect microvascular responses to provocations in the skin. TiVi was found more sensitive for measurement of noradrenaline-induced vasoconstriction, while LSCI was more sensitive for measurement of vascular occlusion. In the second study, microvascular responses in the skin to iontophoresis of vasoactive drugs were found to depend on the drug delivery protocol. Perfusion half-life was defined and used to describe the decay in the microvascular response to a drug after iontophoresis. In the third study, the role of nitric oxide (NO) was assessed during iontophoresis of insulin. The results showed a NO-dependent vasodilation in the skin by insulin. In the fourth study the vasoactive and metabolic effects of insulin were studied after both local and endogenous administration. Local delivery of insulin increased skin blood flow, paralleled by increased skin concentrations of interstitial pyruvate and lactate, although no change in glucose concentration was observed. An oral glucose load resulted in an increased insulin concentration in the skin paralleled by an increase in blood flow, as measured using the microdialysis urea clearance technique, although no changes in perfusion was measured by LSCI. The thesis concludes that when studying skin microvascular responses, the choice of measurement technique and the drug delivery protocol has an impact on the measurement results, and should therefore be carefully considered. The thesis also concludes that insulin has metabolic and vasodilatory effects in the skin both when administered locally and as an endogenous response to an oral glucose load. The vasodilatory effect of insulin in the skin is mediated by nitric oxide

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“…1 LSCI is user-friendly and shows very good reproducibility as well as excellent spatial and temporal resolutions. 2 In clinical studies, LSCI coupled with reactivity tests enable to estimate the endothelial and neurovascular function.…”

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confidence: 99%

Laser speckle contrast imaging of Raynaud phenomenon

Hellmann¹,

Cracowski²

2014

Polish Archives of Internal Medicine

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483peripheral microcirculatory blood flow on a large area of the body. 1 LSCI is user-friendly and shows very good reproducibility as well as excellent spatial and temporal resolutions.2 In clinical studies, LSCI coupled with reactivity tests enable to estimate the endothelial and neurovascular function.We present the measurements of skin perfusion assessed by LSCI in patients with primary and secondary Raynaud phenomenon. FIGURE 1 shows skin flux derived from speckle contrast analysis with colors ranging from blue (no perfusion) to red (high perfusion). LSCI provides a perfusion index proportional to skin blood flow and allows to record up to 100 images per second. FIGURE 1A presents a clear lack of perfusion in the index finger in a patient with primary Raynaud phenomenon. FIGURE 1B presents the hand of a patient with scleroderma. We can see ulcers in Raynaud phenomenon is an episodic vasospasm of the peripheral microvessels in response to cold or stress. It can be primary, or less frequently secondary, to a systemic disease, the most frequent being scleroderma. The pathophysiology is most likely related to abnormal digital microvascular sensitivity to sympathetic stimulation that may also involve an abnormal response of the endothelium. Owing to its small size and regional heterogeneity, studies of the skin microcirculation remain challenging and the only routine method is videocapillaroscopy for evaluating microvascular structure. In addition, skin microvascular dysfunction has been described in many cardiovascular disorders.Laser speckle contrast imaging (LSCI) is a recently developed technique that allows noninvasive, noncontact, and real-time monitoring of

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Excellent reproducibility of laser speckle contrast imaging to assess skin microvascular reactivity

Cited by 249 publications

References 17 publications

Analysis of Laser Speckle Contrast Images Variability Using a Novel Empirical Mode Decomposition: Comparison of Results With Laser Doppler Flowmetry Signals Variability

Analysis of Laser Speckle Contrast Images Variability Using a Novel Empirical Mode Decomposition: Comparison of Results With Laser Doppler Flowmetry Signals Variability

Assessment of microvascular and metabolic responses in the skin

Laser speckle contrast imaging of Raynaud phenomenon

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