Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions

Furuichi, Yukio; Kawabata, Yuki; Aoki, Miho; Mita, Yoshitaka; Fujii, Nobuharu; Manabe, Yasuko

doi:10.3389/fcell.2021.640399

Cited by 42 publications

(29 citation statements)

References 39 publications

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“…However, data suggest that high glucose concentrations may induce SC lipogenesis [ 19 , 20 ]. Recently, data suggest that excess glucose can even impede SC proliferation when comparing a serum-free DMEM and HGDMEM culture media [ 21 ]. While our data are not consistent with Furuichi and colleagues, as they do not reveal decreased proliferation in higher glucose containing basal media, they may indicate that the use of LGDMEM is more optimal than the HGDMEM media as both DMEM media supported MRF+ cells in proliferation and fusion of SC in differentiation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Basal Culture Media and Sera Type Combinations on Primary Broiler Chicken Muscle Satellite Cell Heterogeneity during Proliferation and Differentiation

Flees

Starkey

2022

Animals

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The objective of this experiment was to access primary satellite cell (SC) proliferation and differentiation when cultured in different combinations of basal media and sera due to little consistency being published on the optimal culture media for primary broiler chicken satellite cells. Cells were cultured in one of three different basal media: McCoy’s 5A, high glucose Dulbecco’s Modified Eagle’s medium (DMEM), and low glucose DMEM. Media were supplemented with 15% chicken serum (CS) or a combination of 5% horse serum (HS) + 10% CS during proliferation while 3% HS or 3% CS were added to the media during differentiation. Cultures were immunofluorescence stained for myogenic regulatory factors (MRF) at 48, 72, and 96 h post-plating for proliferation (Pax7, MyoD, and Myf-5) and 96 h post-proliferation during differentiation (Pax7 and MyoD), including MF20 to assess fusion. Cells cultured in Dulbecco’s Modified Eagle’s medium tended to have higher proportions of myogenic cells expressing MRF during proliferation and promoted fusion into myotubes compared with McCoy’s 5A during differentiation. Culturing primary SC in low glucose media, glucose concentrations similar to circulating glucose concentrations in broilers, HSCS during proliferation and CS during differentiation, appears to be optimal for promoting broiler chicken satellite cell proliferation and differentiation.

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Section: Discussionmentioning

confidence: 99%

Effect of Different Basal Culture Media and Sera Type Combinations on Primary Broiler Chicken Muscle Satellite Cell Heterogeneity during Proliferation and Differentiation

Flees

Starkey

2022

Animals

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“…In a cell model using mouse myoblasts C2C12, high glucose exposure of 25 mM was shown to accelerate myogenesis by rearranging SUMO enzyme transcripts and SUMO proteins [55]. However, other experiments with C2C12 have shown that even higher glucose concentrations of 60 mM inhibit the expression of the MyoD and myogenin genes, as well as the Akt signal, suppressing skeletal muscle differentiation [56]. High glucose was also shown to interfere with the proliferation of muscle-specific stem cells and satellite cells under adherent culture conditions [57].…”

Section: Intrauterine Hyperglycaemia-mimicking Cell Modelmentioning

confidence: 95%

Future Risks for Children Born to Mothers with Gestational Diabetes: Elucidation Using the Cell Model Approach

Kawaharada¹,

Nakamura²

2022

Gestational Diabetes Mellitus - New Developments

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A number of studies have shown that foetal nutritional status significantly impacts an unborn child’s long-term health. The developmental origins of health and disease (DOHaD) hypothesis proposes that if a child is undernourished in the foetal period, the child will develop diabetes and hypertension in the future if adequate nutrition is given after birth. Moreover, hyperglycaemia (e.g. gestational diabetes mellitus [GDM]) experienced during foetal life can reportedly cause various complications in children. As diabetes is increasing worldwide, so is GDM, and many studies have been conducted using GDM animal models and GDM cell lines. We examined the effects of streptozotocin-induced diabetes, particularly on the heart of offspring, in rat GDM animal models. We also analysed primary cardiomyocyte cultures isolated from these GDM rats and found that insulin signalling was inhibited in GDM cells, as in the GDM animal models, by increased advanced glycation end products. Furthermore, the effect of eicosapentaenoic acid during pregnancy has been reported in GDM animal models and cells, and the findings indicated the importance of nutritional management for GDM during pregnancy.

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“…Satellite cells were cultured according to our previous report 49 but with some modifications. Briefly, each type of muscle fibre was pooled and incubated with Accutase (Innovative Cell Technologies, SAN, USA) for 10 min at room temperature and cultured on Matrigel-coated dishes.…”

Section: Methodsmentioning

confidence: 99%

R-spondin3 is a myokine that differentiates myoblasts to type I fibres

Mita

Zhu

Furuichi

et al. 2022

Sci Rep

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Muscle fibres are broadly categorised into types I and II; the fibre-type ratio determines the contractile and metabolic properties of skeletal muscle tissue. The maintenance of type I fibres is essential for the prevention of obesity and the treatment of muscle atrophy caused by type 2 diabetes or unloading. Some reports suggest that myokines are related to muscle fibre type determination. We thus explored whether a myokine determines whether satellite cells differentiate to type I fibres. By examining the fibre types separately, we identified R-spondin 3 (Rspo3) as a myokine of interest, a secreted protein known as an activator of Wnt signalling pathways. To examine whether Rspo3 induces type I fibres, primary myoblasts prepared from mouse soleus muscles were exposed to a differentiation medium containing the mouse recombinant Rspo3 protein. Expression of myosin heavy chain (MyHC) I, a marker of type I fibre, significantly increased in the differentiated myotubes compared with a control. The Wnt/β-catenin pathway was shown to be the dominant signalling pathway which induces Rspo3-induced MyHC I expression. These results revealed Rspo3 as a myokine that determines whether satellite cells differentiate to type I fibres.

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Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions

Cited by 42 publications

References 39 publications

Effect of Different Basal Culture Media and Sera Type Combinations on Primary Broiler Chicken Muscle Satellite Cell Heterogeneity during Proliferation and Differentiation

Effect of Different Basal Culture Media and Sera Type Combinations on Primary Broiler Chicken Muscle Satellite Cell Heterogeneity during Proliferation and Differentiation

Future Risks for Children Born to Mothers with Gestational Diabetes: Elucidation Using the Cell Model Approach

R-spondin3 is a myokine that differentiates myoblasts to type I fibres

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