Excessive amount of lactose in the diet of two-week-old calves induces urinary protein changes

Dratwa-Chałupnik, Alicja; Ożgo, M.; Lepczyński, Adam; Herosimczyk, Agnieszka; Michałek, Katarzyna

doi:10.5194/aab-59-417-2016

Cited by 4 publications

(5 citation statements)

References 29 publications

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“…The results of the previous studies [11] indicate that an excessive amount of lactose in a diet of calves caused osmotic diarrhea. Proteomic analysis of the urine showed changes in the protein profile after administration of lactose to the milk replacer.…”

Section: Urinary Proteomics In Veterinary Medicinementioning

confidence: 96%

“…The animals were under veterinary care and supervision and that they did not show any clinical symptoms of diseases. During the first three days of life, calves were fed with colostrum and then from the 4th day with milk replacer (see Dratwa-Chałupnik et al [11] for details). Each day the animals were weighed, because the amount of milk replacer was given per calf body weight.…”

Section: Animals and Dietsmentioning

confidence: 99%

“…Moreover, analysis of the camel urinary proteome allowed for the identification of proteins involved in various stress and immune response processes, as well as those displaying antimicrobial activities [10]. Our previous study showed that excessive amounts of lactose in the diet of two-week-old calves resulted in altered urinary protein profile [11].…”

Section: Introductionmentioning

confidence: 99%

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Urinary Proteome of Newborn Calves—New Potential in Non-Invasive Neonatal Diagnostic

Dratwa-Chałupnik

Wojdyła

Ożgo

et al. 2020

Animals

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Urine is a biological diagnostic material suitable not only for the analysis of kidney and urinary tract functions but also the function of other tissues and organs. The urine proteome of adult mammals differs from the urine proteome of neonatal ones. The establishment of urinary protein maps of healthy newborn calves is important for diagnosing and monitoring the progression of various diseases. The experiment was carried out on a Polish-Friesian var. of Black-and-White male calves in the sixth day of postnatal life. The two proteomics approaches used for separation and identification of urinary proteins were: 2-DE with MALDI-TOF-TOF-MS/MS and 1-DE with LC-MS/MS. This resulted in the identification of 692 urinary proteins. The majority of them were classified as extracellular proteins (40.32%), as well as proteins involved in regulation of major cellular processes (31.07%). We have observed the presence of unique proteins associated with embryonic (ameloblastin, alpha-fetoprotein, Delta-like protein, embryo-specific fibronectin 1 transcript variant, Indian hedgehog homolog) and kidney development (angiotensin-converting enzyme, angiotensinogen, aquaporin-1, calbindin, glypican 3, nidogen 1, pro-cathepsin H). Additionally, proteins involved in the renal regulation of water and electrolyte balance (angiotensinogen, angiotensin-converting enzyme, aquaporin-1, ezrin, uromodulin) were detected. Presented in the current study 1-D and 2-D urinary proteomic maps are the basis for the identification and detection of prognostic biomarkers important for defining a calf’s health status.

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Section: Urinary Proteomics In Veterinary Medicinementioning

confidence: 96%

Section: Animals and Dietsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Urinary Proteome of Newborn Calves—New Potential in Non-Invasive Neonatal Diagnostic

Dratwa-Chałupnik

Wojdyła

Ożgo

et al. 2020

Animals

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“…The migration of proteins was run at 40 V for 3.5 hours and 90 V for 14 hours. After migration, the mass spectrometry identification was performed according to Dratwa-Chałupnik et al [2016] procedure.…”

Section: Two-dimensional Electrophoresis and Identification Of The Proteinsmentioning

confidence: 99%

Identification of mare colostrum proteins

Medeńska¹,

Dratwa-Chałupnik²,

Ożgo³

et al. 2021

Acta Sci. Pol. Zootech.

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Colostrum is an essential feed of foals. It is a source of nutrients and functional proteins significant for foals’ growth and development. In the presented research using two-dimensional electrophoresis coupled via spectrometry mass MALDI-TOF in the mares’ colostrum (whey proteins fraction) were identified 24 proteins representing 15 different gene products. The identified proteins were involved in supporting foals’ immature immune systems and in the transport of various compounds. Further research of mares’ colostrum will allow determining more gene products. An in-depth analysis of mares’ milk will provide information about biochemical processes occurring in the mammary gland of the mare during the lactation period.

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“…Comparison of the urinary protein profiles between cows and heifers has detected the protein changes occurring in pregnancy progresses 15 . Altered urinary proteomes are detected in calves after feeding different amounts of lactose in diet, which indicates calves’ urine a valuable biological material to evaluate renal adjustment to various physiological factors 16 . Urinary protein composition of normal neonatal rats has been compared to adults in one study 17 .…”

Section: Introductionmentioning

confidence: 99%

DIA mass spectrometry characterizes urinary proteomics in neonatal and adult donkeys

Feng

Chen

Liu

et al. 2022

Sci Rep

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Health monitoring is critical for newborn animals due to their vulnerability to diseases. Urine can be not only a useful and non-invasive tool (free-catch samples) to reflect the physiological status of animals but also to help monitor the progression of diseases. Proteomics involves the study of the whole complement of proteins and peptides, including structure, quantities, functions, variations and interactions. In this study, urinary proteomics of neonatal donkeys were characterized and compared to the profiles of adult donkeys to provide a reference database for healthy neonatal donkeys. The urine samples were collected from male neonatal donkeys on their sixth to tenth days of life (group N) and male adult donkeys aging 4–6 years old (group A). Library-free data-independent acquisition (direct DIA) mass spectrometry-based proteomics were applied to analyze the urinary protein profiles. Total 2179 urinary proteins were identified, and 411 proteins were differentially expressed (P < 0.05) between the two groups. 104 proteins were exclusively expressed in group N including alpha fetoprotein (AFP), peptidase-mitochondrial processing data unit (PMPCB), and upper zone of growth plate and cartilage matrix associated (UCMA), which might be used to monitor the health status of neonatal donkeys. In functional analysis, some differentially expressed proteins were identified related to immune system pathways, which might provide more insight in the immature immunity of neonatal donkeys. To the best of our knowledge, this is the first time to report donkey urinary proteome and our results might provide reference for urinary biomarker discovery used to monitor and evaluate health status of neonatal donkeys.

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Excessive amount of lactose in the diet of two-week-old calves induces urinary protein changes

Cited by 4 publications

References 29 publications

Urinary Proteome of Newborn Calves—New Potential in Non-Invasive Neonatal Diagnostic

Urinary Proteome of Newborn Calves—New Potential in Non-Invasive Neonatal Diagnostic

Identification of mare colostrum proteins

DIA mass spectrometry characterizes urinary proteomics in neonatal and adult donkeys

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