Excessive chromosome fragility and abundance of sister-chromatid exchanges induced by UV in an Indian muntjac cell line defective in postreplication (daughter strand) repair

Pillidge, L.; Musk, S. R. R.; Johnson, Robert T.; Waldren, Charles A.

doi:10.1016/0167-8817(86)90026-x

Mutation Research/DNA Repair Reports

1986

DOI: 10.1016/0167-8817(86)90026-x

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Excessive chromosome fragility and abundance of sister-chromatid exchanges induced by UV in an Indian muntjac cell line defective in postreplication (daughter strand) repair

L. Pillidge¹,

S. R. R. Musk²,

Robert T. Johnson³

et al.

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1987

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Cited by 5 publications

References 27 publications

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Scanning electron microscope analysis of structural changes and aberrations in human chromosomes associated with the inhibition and reversal of inhibition of ultraviolet light induced DNA repair

Mullinger

Johnson

1987

Chromosoma

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Metaphase chromosomes appear decondensed in preparations from mitotic cells that have been irradiated with ultraviolet light (UV) and incubated with inhibitors of DNA synthesis; under these conditions DNA repair is inhibited and both single and double strand DNA breaks accumulate. After reversal of the inhibition chromosomes are condensed, but are often damaged. In this paper we show by scanning electron microscopy (SEM) that decondensed HeLa chromosomes are composed of fibre clusters similar to those previously described for the large chromosomes of the Indian muntjac. This suggests that the clusters may be a universal higher order packing unit in mammalian metaphase chromosomes. We also examine by SEM the nature of the aberrations that appear after the reversal of periods of inhibited repair; these include gaps, breaks, deletions and telomeric packing abnormalities. SEM analysis allows an extension and reconsideration of conclusions about chromatid continuity based on the study of conventional light microscope (LM) preparations.

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Scanning electron microscope analysis of structural changes and aberrations in human chromosomes associated with the inhibition and reversal of inhibition of ultraviolet light induced DNA repair

Mullinger

Johnson

1987

Chromosoma

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Dominant genetic instability and sensitivity to DNA damaging agents in a mammalian cell line

Ryan

Johnson

1996

Somat Cell Mol Genet

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An SV40 transformed Indian muntjac cell line (SVM) has been shown to be hypersensitive to cell killing by a wide range of DNA damaging agents. Evidence points to defects in DNA replication and DNA recombination resulting in chromosome instability both spontaneously and following exposure to DNA damaging agents. We have generated proliferating hybrids between SVM and a spontaneously transformed Indian muntjac cell line (DM). Study of these hybrids indicates that the SVM phenotype acts in a genetically dominant manner and is associated with the expression of SV40 large T antigen. We propose that transformation and immortalization of Indian muntjac fibroblasts by SV40 virus can lead to a set of persistent changes in gene expression that result in chromosome instability and increased sensitivity to DNA damaging agents. Genes involved in these processes are likely to be of great importance as chromosome instability can play a central role in cancer development.

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The clastogenic effects of isothiocyanates

Musk

Johnson

1993

Mutation Research/Genetic Toxicology

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Excessive chromosome fragility and abundance of sister-chromatid exchanges induced by UV in an Indian muntjac cell line defective in postreplication (daughter strand) repair

Cited by 5 publications

References 27 publications

Scanning electron microscope analysis of structural changes and aberrations in human chromosomes associated with the inhibition and reversal of inhibition of ultraviolet light induced DNA repair

Scanning electron microscope analysis of structural changes and aberrations in human chromosomes associated with the inhibition and reversal of inhibition of ultraviolet light induced DNA repair

Dominant genetic instability and sensitivity to DNA damaging agents in a mammalian cell line

The clastogenic effects of isothiocyanates

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